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Curvature-dependent constraints drive remodeling of epithelia
Epithelial tissues function as barriers that separate the organism from the environment. They usually have highly curved shapes, such as tubules or cysts. However, the processes by which the geometry of the environment and the cell's mechanical properties set the epithelium shape are not yet kn...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398478/ https://www.ncbi.nlm.nih.gov/pubmed/30578312 http://dx.doi.org/10.1242/jcs.222372 |
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author | Maechler, Florian A. Allier, Cédric Roux, Aurélien Tomba, Caterina |
author_facet | Maechler, Florian A. Allier, Cédric Roux, Aurélien Tomba, Caterina |
author_sort | Maechler, Florian A. |
collection | PubMed |
description | Epithelial tissues function as barriers that separate the organism from the environment. They usually have highly curved shapes, such as tubules or cysts. However, the processes by which the geometry of the environment and the cell's mechanical properties set the epithelium shape are not yet known. In this study, we encapsulated two epithelial cell lines, MDCK and J3B1A, into hollow alginate tubes and grew them under cylindrical confinement forming a complete monolayer. MDCK monolayers detached from the alginate shell at a constant rate, whereas J3B1A monolayers detached at a low rate unless the tube radius was reduced. We showed that this detachment is driven by contractile stresses in the epithelium and can be enhanced by local curvature. This allows us to conclude that J3B1A cells exhibit smaller contractility than MDCK cells. Monolayers inside curved tubes detach at a higher rate on the outside of a curve, confirming that detachment is driven by contraction. |
format | Online Article Text |
id | pubmed-6398478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-63984782019-03-21 Curvature-dependent constraints drive remodeling of epithelia Maechler, Florian A. Allier, Cédric Roux, Aurélien Tomba, Caterina J Cell Sci Research Article Epithelial tissues function as barriers that separate the organism from the environment. They usually have highly curved shapes, such as tubules or cysts. However, the processes by which the geometry of the environment and the cell's mechanical properties set the epithelium shape are not yet known. In this study, we encapsulated two epithelial cell lines, MDCK and J3B1A, into hollow alginate tubes and grew them under cylindrical confinement forming a complete monolayer. MDCK monolayers detached from the alginate shell at a constant rate, whereas J3B1A monolayers detached at a low rate unless the tube radius was reduced. We showed that this detachment is driven by contractile stresses in the epithelium and can be enhanced by local curvature. This allows us to conclude that J3B1A cells exhibit smaller contractility than MDCK cells. Monolayers inside curved tubes detach at a higher rate on the outside of a curve, confirming that detachment is driven by contraction. The Company of Biologists Ltd 2019-02-15 2019-01-24 /pmc/articles/PMC6398478/ /pubmed/30578312 http://dx.doi.org/10.1242/jcs.222372 Text en © 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Maechler, Florian A. Allier, Cédric Roux, Aurélien Tomba, Caterina Curvature-dependent constraints drive remodeling of epithelia |
title | Curvature-dependent constraints drive remodeling of epithelia |
title_full | Curvature-dependent constraints drive remodeling of epithelia |
title_fullStr | Curvature-dependent constraints drive remodeling of epithelia |
title_full_unstemmed | Curvature-dependent constraints drive remodeling of epithelia |
title_short | Curvature-dependent constraints drive remodeling of epithelia |
title_sort | curvature-dependent constraints drive remodeling of epithelia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398478/ https://www.ncbi.nlm.nih.gov/pubmed/30578312 http://dx.doi.org/10.1242/jcs.222372 |
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