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The impact of urbanisation on community structure, gene abundance and transcription rates of microbes in upland swamps of Eastern Australia

The Temperate Highland Peat Swamps on Sandstone of the Sydney Basin occur in the headwaters of Sydney’s drinking water catchments and are listed as endangered ecosystems, yet they have suffered habitat losses and degradation due to human impacts such as urbanisation. Despite ongoing efforts to resto...

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Autores principales: Christiansen, Nicole A., Fryirs, Kirstie A., Green, Timothy J., Hose, Grant C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398846/
https://www.ncbi.nlm.nih.gov/pubmed/30830948
http://dx.doi.org/10.1371/journal.pone.0213275
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author Christiansen, Nicole A.
Fryirs, Kirstie A.
Green, Timothy J.
Hose, Grant C.
author_facet Christiansen, Nicole A.
Fryirs, Kirstie A.
Green, Timothy J.
Hose, Grant C.
author_sort Christiansen, Nicole A.
collection PubMed
description The Temperate Highland Peat Swamps on Sandstone of the Sydney Basin occur in the headwaters of Sydney’s drinking water catchments and are listed as endangered ecosystems, yet they have suffered habitat losses and degradation due to human impacts such as urbanisation. Despite ongoing efforts to restore and better protect upland swamps, they remain poorly understood, potentially hindering the effectiveness of management efforts. Essential to overall ecosystem function and the provision of services for human and environmental benefit are the microbial component of wetland ecosystems. In the case of these swamps, the microbes, have not yet been studied. Here, we investigated differences in the microbial community of upland swamps in urbanised catchments compared to swamps from natural catchments in the Blue Mountains. A total of twelve swamps were sampled, six from within urbanised catchments and six with intact vegetation catchments, to compare sediment conditions and microbial community and genes expression and abundances. Catchment impervious area and number of stormwater drains entering a swamp, indicators for urbanisation, positively correlated with the pH and ammonium concentration of swamp sediment. Community analysis of the 16S rRNA gene (T-RFLP, qPCR) revealed the elevated pH of urbanised swamps coincided with changes to the abundance of bacteria and archaea. Furthermore, RT-qPCR revealed genes involved in carbon cycling (mcrA & pmoA) were more likely to be found in urbanised swamps. Taken together, our results indicate that urbanisation of the Blue Mountains is impacting the environmental services provided by the microbial community of upland swamps in the Sydney Basin.
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spelling pubmed-63988462019-03-08 The impact of urbanisation on community structure, gene abundance and transcription rates of microbes in upland swamps of Eastern Australia Christiansen, Nicole A. Fryirs, Kirstie A. Green, Timothy J. Hose, Grant C. PLoS One Research Article The Temperate Highland Peat Swamps on Sandstone of the Sydney Basin occur in the headwaters of Sydney’s drinking water catchments and are listed as endangered ecosystems, yet they have suffered habitat losses and degradation due to human impacts such as urbanisation. Despite ongoing efforts to restore and better protect upland swamps, they remain poorly understood, potentially hindering the effectiveness of management efforts. Essential to overall ecosystem function and the provision of services for human and environmental benefit are the microbial component of wetland ecosystems. In the case of these swamps, the microbes, have not yet been studied. Here, we investigated differences in the microbial community of upland swamps in urbanised catchments compared to swamps from natural catchments in the Blue Mountains. A total of twelve swamps were sampled, six from within urbanised catchments and six with intact vegetation catchments, to compare sediment conditions and microbial community and genes expression and abundances. Catchment impervious area and number of stormwater drains entering a swamp, indicators for urbanisation, positively correlated with the pH and ammonium concentration of swamp sediment. Community analysis of the 16S rRNA gene (T-RFLP, qPCR) revealed the elevated pH of urbanised swamps coincided with changes to the abundance of bacteria and archaea. Furthermore, RT-qPCR revealed genes involved in carbon cycling (mcrA & pmoA) were more likely to be found in urbanised swamps. Taken together, our results indicate that urbanisation of the Blue Mountains is impacting the environmental services provided by the microbial community of upland swamps in the Sydney Basin. Public Library of Science 2019-03-04 /pmc/articles/PMC6398846/ /pubmed/30830948 http://dx.doi.org/10.1371/journal.pone.0213275 Text en © 2019 Christiansen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Christiansen, Nicole A.
Fryirs, Kirstie A.
Green, Timothy J.
Hose, Grant C.
The impact of urbanisation on community structure, gene abundance and transcription rates of microbes in upland swamps of Eastern Australia
title The impact of urbanisation on community structure, gene abundance and transcription rates of microbes in upland swamps of Eastern Australia
title_full The impact of urbanisation on community structure, gene abundance and transcription rates of microbes in upland swamps of Eastern Australia
title_fullStr The impact of urbanisation on community structure, gene abundance and transcription rates of microbes in upland swamps of Eastern Australia
title_full_unstemmed The impact of urbanisation on community structure, gene abundance and transcription rates of microbes in upland swamps of Eastern Australia
title_short The impact of urbanisation on community structure, gene abundance and transcription rates of microbes in upland swamps of Eastern Australia
title_sort impact of urbanisation on community structure, gene abundance and transcription rates of microbes in upland swamps of eastern australia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398846/
https://www.ncbi.nlm.nih.gov/pubmed/30830948
http://dx.doi.org/10.1371/journal.pone.0213275
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