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Isolation, Characterization and Amino Acid Composition of a Bacteriocin Produced by Bacillus methylotrophicus Strain BM47

Members of the bacterial genus Bacillus are known as producers of a broad spectrum of antibiotic compounds of proteinaceous nature that possess inhibitory activity against different saprophytic and pathogenic microorganisms. In the current research, a peptide synthesized by Bacillus methylotrophicus...

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Autores principales: Tumbarski, Yulian, Deseva, Ivelina, Mihaylova, Dasha, Stoyanova, Magdalena, Krastev, Lutsian, Nikolova, Radosveta, Yanakieva, Velichka, Ivanov, Ivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: University of Zagreb Faculty of Food Technology and Biotechnology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399719/
https://www.ncbi.nlm.nih.gov/pubmed/30923451
http://dx.doi.org/10.17113/ftb.56.04.18.5905
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author Tumbarski, Yulian
Deseva, Ivelina
Mihaylova, Dasha
Stoyanova, Magdalena
Krastev, Lutsian
Nikolova, Radosveta
Yanakieva, Velichka
Ivanov, Ivan
author_facet Tumbarski, Yulian
Deseva, Ivelina
Mihaylova, Dasha
Stoyanova, Magdalena
Krastev, Lutsian
Nikolova, Radosveta
Yanakieva, Velichka
Ivanov, Ivan
author_sort Tumbarski, Yulian
collection PubMed
description Members of the bacterial genus Bacillus are known as producers of a broad spectrum of antibiotic compounds of proteinaceous nature that possess inhibitory activity against different saprophytic and pathogenic microorganisms. In the current research, a peptide synthesized by Bacillus methylotrophicus strain BM47, previously isolated from a natural thermal spring in Bulgaria, was identified and characterized as a bacteriocin. In vitro antimicrobial screening of the crude bacteriocin substance of B. methylotrophicus BM47 showed activity against the plant pathogenic fungi Fusarium moniliforme, Aspergillus awamori, Penicillium sp., Aspergillus niger and Gram-negative bacterium Pseudomonas aeruginosa. The antimicrobial activity of the crude bacteriocin substance was partially inhibited by the enzymes trypsin, Alcalase®, Savinase®, proteinase K, papain and Esperase®, while catalase was not effective. The crude bacteriocin substance was relatively pH resistant, but sensitive to the action of heat and most organic solvents and detergents tested. To obtain the active protein fractions, crude bacteriocin substance was purified by fast protein liquid chromatography (FPLC) using a strong anion exchange column. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis demonstrated that the purified bacteriocin had molecular mass of 19 578 Da. The amino acid analysis performed by high-performance liquid chromatography (HPLC) revealed that the isolated bacteriocin consisted of 17 types of amino acids, with the highest mol fraction expressed as percent of serine (29.3), valine (10.3), alanine (9.8) and tyrosine (7.1).
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spelling pubmed-63997192019-03-28 Isolation, Characterization and Amino Acid Composition of a Bacteriocin Produced by Bacillus methylotrophicus Strain BM47 Tumbarski, Yulian Deseva, Ivelina Mihaylova, Dasha Stoyanova, Magdalena Krastev, Lutsian Nikolova, Radosveta Yanakieva, Velichka Ivanov, Ivan Food Technol Biotechnol Original Scientific Papers Members of the bacterial genus Bacillus are known as producers of a broad spectrum of antibiotic compounds of proteinaceous nature that possess inhibitory activity against different saprophytic and pathogenic microorganisms. In the current research, a peptide synthesized by Bacillus methylotrophicus strain BM47, previously isolated from a natural thermal spring in Bulgaria, was identified and characterized as a bacteriocin. In vitro antimicrobial screening of the crude bacteriocin substance of B. methylotrophicus BM47 showed activity against the plant pathogenic fungi Fusarium moniliforme, Aspergillus awamori, Penicillium sp., Aspergillus niger and Gram-negative bacterium Pseudomonas aeruginosa. The antimicrobial activity of the crude bacteriocin substance was partially inhibited by the enzymes trypsin, Alcalase®, Savinase®, proteinase K, papain and Esperase®, while catalase was not effective. The crude bacteriocin substance was relatively pH resistant, but sensitive to the action of heat and most organic solvents and detergents tested. To obtain the active protein fractions, crude bacteriocin substance was purified by fast protein liquid chromatography (FPLC) using a strong anion exchange column. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis demonstrated that the purified bacteriocin had molecular mass of 19 578 Da. The amino acid analysis performed by high-performance liquid chromatography (HPLC) revealed that the isolated bacteriocin consisted of 17 types of amino acids, with the highest mol fraction expressed as percent of serine (29.3), valine (10.3), alanine (9.8) and tyrosine (7.1). University of Zagreb Faculty of Food Technology and Biotechnology 2018-12 /pmc/articles/PMC6399719/ /pubmed/30923451 http://dx.doi.org/10.17113/ftb.56.04.18.5905 Text en http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial (CC BY-NC) 4.0 License.
spellingShingle Original Scientific Papers
Tumbarski, Yulian
Deseva, Ivelina
Mihaylova, Dasha
Stoyanova, Magdalena
Krastev, Lutsian
Nikolova, Radosveta
Yanakieva, Velichka
Ivanov, Ivan
Isolation, Characterization and Amino Acid Composition of a Bacteriocin Produced by Bacillus methylotrophicus Strain BM47
title Isolation, Characterization and Amino Acid Composition of a Bacteriocin Produced by Bacillus methylotrophicus Strain BM47
title_full Isolation, Characterization and Amino Acid Composition of a Bacteriocin Produced by Bacillus methylotrophicus Strain BM47
title_fullStr Isolation, Characterization and Amino Acid Composition of a Bacteriocin Produced by Bacillus methylotrophicus Strain BM47
title_full_unstemmed Isolation, Characterization and Amino Acid Composition of a Bacteriocin Produced by Bacillus methylotrophicus Strain BM47
title_short Isolation, Characterization and Amino Acid Composition of a Bacteriocin Produced by Bacillus methylotrophicus Strain BM47
title_sort isolation, characterization and amino acid composition of a bacteriocin produced by bacillus methylotrophicus strain bm47
topic Original Scientific Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399719/
https://www.ncbi.nlm.nih.gov/pubmed/30923451
http://dx.doi.org/10.17113/ftb.56.04.18.5905
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