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Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador

OBJECTIVE: Emergence and dissemination of colistin-resistant (Co-R) bacteria harboring mobile colistin resistance genes pose a threat for treatment of infections caused by multi-drug resistant bacteria. Although the worldwide spread of Co-R bacteria is known, the precise state of Co-R bacterial diss...

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Autores principales: Yamamoto, Yoshimasa, Calvopina, Manuel, Izurieta, Ricardo, Villacres, Irina, Kawahara, Ryuji, Sasaki, Masahiro, Yamamoto, Mayumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399824/
https://www.ncbi.nlm.nih.gov/pubmed/30832731
http://dx.doi.org/10.1186/s13104-019-4144-0
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author Yamamoto, Yoshimasa
Calvopina, Manuel
Izurieta, Ricardo
Villacres, Irina
Kawahara, Ryuji
Sasaki, Masahiro
Yamamoto, Mayumi
author_facet Yamamoto, Yoshimasa
Calvopina, Manuel
Izurieta, Ricardo
Villacres, Irina
Kawahara, Ryuji
Sasaki, Masahiro
Yamamoto, Mayumi
author_sort Yamamoto, Yoshimasa
collection PubMed
description OBJECTIVE: Emergence and dissemination of colistin-resistant (Co-R) bacteria harboring mobile colistin resistance genes pose a threat for treatment of infections caused by multi-drug resistant bacteria. Although the worldwide spread of Co-R bacteria is known, the precise state of Co-R bacterial dissemination in livestock of Andean countries remains unclear. Therefore, we investigated mcr-containing Co-R Escherichia coli dissemination in livestock on small-scale farms in two socioecologically different regions of Ecuador: the Amazonian rain-forest and the Pacific Coast. RESULTS: Sixty-six rectal swab samples from 34 pigs and 32 chickens, from five farms in the two regions, were assessed for the dissemination of Co-R E. coli using the selective medium CHROMagar™ COL-APSE. mcr-containing Co-R E. coli were detected in the specimens at a high rate (47%; 31/66), but the detection rates of the two regions were not statistically different. Both chickens and pigs showed similar detection rates. All Co-R E. coli isolates harbored mcr-1. The minimum inhibitory concentrations of colistin were ≥ 8 mg/L, and 67.7% (21/31) of the Co-R isolates were multi-drug resistant. Pulsed-field gel electrophoresis revealed the limited relation between isolates. Thus, we revealed the high rate of widespread dissemination of Co-R bacteria in livestock regardless of the socioecological conditions in Ecuador.
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spelling pubmed-63998242019-03-13 Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador Yamamoto, Yoshimasa Calvopina, Manuel Izurieta, Ricardo Villacres, Irina Kawahara, Ryuji Sasaki, Masahiro Yamamoto, Mayumi BMC Res Notes Research Note OBJECTIVE: Emergence and dissemination of colistin-resistant (Co-R) bacteria harboring mobile colistin resistance genes pose a threat for treatment of infections caused by multi-drug resistant bacteria. Although the worldwide spread of Co-R bacteria is known, the precise state of Co-R bacterial dissemination in livestock of Andean countries remains unclear. Therefore, we investigated mcr-containing Co-R Escherichia coli dissemination in livestock on small-scale farms in two socioecologically different regions of Ecuador: the Amazonian rain-forest and the Pacific Coast. RESULTS: Sixty-six rectal swab samples from 34 pigs and 32 chickens, from five farms in the two regions, were assessed for the dissemination of Co-R E. coli using the selective medium CHROMagar™ COL-APSE. mcr-containing Co-R E. coli were detected in the specimens at a high rate (47%; 31/66), but the detection rates of the two regions were not statistically different. Both chickens and pigs showed similar detection rates. All Co-R E. coli isolates harbored mcr-1. The minimum inhibitory concentrations of colistin were ≥ 8 mg/L, and 67.7% (21/31) of the Co-R isolates were multi-drug resistant. Pulsed-field gel electrophoresis revealed the limited relation between isolates. Thus, we revealed the high rate of widespread dissemination of Co-R bacteria in livestock regardless of the socioecological conditions in Ecuador. BioMed Central 2019-03-04 /pmc/articles/PMC6399824/ /pubmed/30832731 http://dx.doi.org/10.1186/s13104-019-4144-0 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Yamamoto, Yoshimasa
Calvopina, Manuel
Izurieta, Ricardo
Villacres, Irina
Kawahara, Ryuji
Sasaki, Masahiro
Yamamoto, Mayumi
Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador
title Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador
title_full Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador
title_fullStr Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador
title_full_unstemmed Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador
title_short Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador
title_sort colistin-resistant escherichia coli with mcr genes in the livestock of rural small-scale farms in ecuador
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399824/
https://www.ncbi.nlm.nih.gov/pubmed/30832731
http://dx.doi.org/10.1186/s13104-019-4144-0
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