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Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography

The ability to determine high-quality, artefact-free structures is a challenge in micro-crystallography, and the rapid onset of radiation damage and requirement for a high-brilliance X-ray beam mean that a multi-crystal approach is essential. However, the combination of crystal-to-crystal variation...

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Autores principales: Ebrahim, Ali, Appleby, Martin V., Axford, Danny, Beale, John, Moreno-Chicano, Tadeo, Sherrell, Darren A., Strange, Richard W., Hough, Michael A., Owen, Robin L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400251/
https://www.ncbi.nlm.nih.gov/pubmed/30821704
http://dx.doi.org/10.1107/S2059798318010240
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author Ebrahim, Ali
Appleby, Martin V.
Axford, Danny
Beale, John
Moreno-Chicano, Tadeo
Sherrell, Darren A.
Strange, Richard W.
Hough, Michael A.
Owen, Robin L.
author_facet Ebrahim, Ali
Appleby, Martin V.
Axford, Danny
Beale, John
Moreno-Chicano, Tadeo
Sherrell, Darren A.
Strange, Richard W.
Hough, Michael A.
Owen, Robin L.
author_sort Ebrahim, Ali
collection PubMed
description The ability to determine high-quality, artefact-free structures is a challenge in micro-crystallography, and the rapid onset of radiation damage and requirement for a high-brilliance X-ray beam mean that a multi-crystal approach is essential. However, the combination of crystal-to-crystal variation and X-ray-induced changes can make the formation of a final complete data set challenging; this is particularly true in the case of metalloproteins, where X-ray-induced changes occur rapidly and at the active site. An approach is described that allows the resolution, separation and structure determination of crystal polymorphs, and the tracking of radiation damage in microcrystals. Within the microcrystal population of copper nitrite reductase, two polymorphs with different unit-cell sizes were successfully separated to determine two independent structures, and an X-ray-driven change between these polymorphs was followed. This was achieved through the determination of multiple serial structures from microcrystals using a high-throughput high-speed fixed-target approach coupled with robust data processing.
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spelling pubmed-64002512019-03-13 Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography Ebrahim, Ali Appleby, Martin V. Axford, Danny Beale, John Moreno-Chicano, Tadeo Sherrell, Darren A. Strange, Richard W. Hough, Michael A. Owen, Robin L. Acta Crystallogr D Struct Biol Research Papers The ability to determine high-quality, artefact-free structures is a challenge in micro-crystallography, and the rapid onset of radiation damage and requirement for a high-brilliance X-ray beam mean that a multi-crystal approach is essential. However, the combination of crystal-to-crystal variation and X-ray-induced changes can make the formation of a final complete data set challenging; this is particularly true in the case of metalloproteins, where X-ray-induced changes occur rapidly and at the active site. An approach is described that allows the resolution, separation and structure determination of crystal polymorphs, and the tracking of radiation damage in microcrystals. Within the microcrystal population of copper nitrite reductase, two polymorphs with different unit-cell sizes were successfully separated to determine two independent structures, and an X-ray-driven change between these polymorphs was followed. This was achieved through the determination of multiple serial structures from microcrystals using a high-throughput high-speed fixed-target approach coupled with robust data processing. International Union of Crystallography 2018-11-09 /pmc/articles/PMC6400251/ /pubmed/30821704 http://dx.doi.org/10.1107/S2059798318010240 Text en © Ebrahim et al. 2019 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/
spellingShingle Research Papers
Ebrahim, Ali
Appleby, Martin V.
Axford, Danny
Beale, John
Moreno-Chicano, Tadeo
Sherrell, Darren A.
Strange, Richard W.
Hough, Michael A.
Owen, Robin L.
Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography
title Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography
title_full Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography
title_fullStr Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography
title_full_unstemmed Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography
title_short Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography
title_sort resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400251/
https://www.ncbi.nlm.nih.gov/pubmed/30821704
http://dx.doi.org/10.1107/S2059798318010240
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