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The Effect of Cortical Elasticity and Active Tension on Cell Adhesion Mechanics
We consider a cell as an elastic, contractile shell surrounding a liquid incompressible cytoplasm and with nonspecific adhesion. We perform numerical simulations of this model to study the mechanics of cell-cell separation. By variation of parameters, we are able to recover well-known limits of the...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400824/ https://www.ncbi.nlm.nih.gov/pubmed/30773295 http://dx.doi.org/10.1016/j.bpj.2019.01.015 |
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author | Smeets, Bart Cuvelier, Maxim Pešek, Jiri Ramon, Herman |
author_facet | Smeets, Bart Cuvelier, Maxim Pešek, Jiri Ramon, Herman |
author_sort | Smeets, Bart |
collection | PubMed |
description | We consider a cell as an elastic, contractile shell surrounding a liquid incompressible cytoplasm and with nonspecific adhesion. We perform numerical simulations of this model to study the mechanics of cell-cell separation. By variation of parameters, we are able to recover well-known limits of the Johnson-Kendall-Roberts theory, the Derjaguin-Muller-Toporov model, adhesive vesicles with surface tension (Brochard-Wyart and de Gennes derivation), and thin elastic shells. We further locate biological cells on this parameter space by comparison to existing experiments on S180 cells. Using this model, we show that mechanical parameters can be obtained that are consistent with both dual pipette aspiration and micropipette aspiration, a problem not successfully tackled so far. We estimate a cortex elastic modulus of E(c) ≈ 15 kPa, an effective cortex thickness of t(c) ≈ 0.3 μm, and an active tension of γ ≈ 0.4 nN/μm. With these parameters, a Johnson-Kendall-Roberts-like scaling of the separation force is recovered. Finally, the change of contact radius with applied force in a pull-off experiment was investigated. For small forces, a scaling similar to both the Brochard-Wyart and de Gennes derivation and the Derjaguin-Muller-Toporov model is found. |
format | Online Article Text |
id | pubmed-6400824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Biophysical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-64008242020-03-05 The Effect of Cortical Elasticity and Active Tension on Cell Adhesion Mechanics Smeets, Bart Cuvelier, Maxim Pešek, Jiri Ramon, Herman Biophys J Articles We consider a cell as an elastic, contractile shell surrounding a liquid incompressible cytoplasm and with nonspecific adhesion. We perform numerical simulations of this model to study the mechanics of cell-cell separation. By variation of parameters, we are able to recover well-known limits of the Johnson-Kendall-Roberts theory, the Derjaguin-Muller-Toporov model, adhesive vesicles with surface tension (Brochard-Wyart and de Gennes derivation), and thin elastic shells. We further locate biological cells on this parameter space by comparison to existing experiments on S180 cells. Using this model, we show that mechanical parameters can be obtained that are consistent with both dual pipette aspiration and micropipette aspiration, a problem not successfully tackled so far. We estimate a cortex elastic modulus of E(c) ≈ 15 kPa, an effective cortex thickness of t(c) ≈ 0.3 μm, and an active tension of γ ≈ 0.4 nN/μm. With these parameters, a Johnson-Kendall-Roberts-like scaling of the separation force is recovered. Finally, the change of contact radius with applied force in a pull-off experiment was investigated. For small forces, a scaling similar to both the Brochard-Wyart and de Gennes derivation and the Derjaguin-Muller-Toporov model is found. The Biophysical Society 2019-03-05 2019-01-24 /pmc/articles/PMC6400824/ /pubmed/30773295 http://dx.doi.org/10.1016/j.bpj.2019.01.015 Text en © 2019 Biophysical Society. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Articles Smeets, Bart Cuvelier, Maxim Pešek, Jiri Ramon, Herman The Effect of Cortical Elasticity and Active Tension on Cell Adhesion Mechanics |
title | The Effect of Cortical Elasticity and Active Tension on Cell Adhesion Mechanics |
title_full | The Effect of Cortical Elasticity and Active Tension on Cell Adhesion Mechanics |
title_fullStr | The Effect of Cortical Elasticity and Active Tension on Cell Adhesion Mechanics |
title_full_unstemmed | The Effect of Cortical Elasticity and Active Tension on Cell Adhesion Mechanics |
title_short | The Effect of Cortical Elasticity and Active Tension on Cell Adhesion Mechanics |
title_sort | effect of cortical elasticity and active tension on cell adhesion mechanics |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400824/ https://www.ncbi.nlm.nih.gov/pubmed/30773295 http://dx.doi.org/10.1016/j.bpj.2019.01.015 |
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