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Analytical Investigation of Cymbopogon citratus and Exploiting the Potential of Developed Silver Nanoparticle Against the Dominating Species of Pathogenic Bacteria

Indian biodiversity is a hub for medicinal plants. Extensive research has been carried out to select plants with numerous properties which can be used for human welfare. Present research is about Cymbopogon citratus, an economically valuable medicinal plant. In this study Cymbopogon citratus was ele...

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Autores principales: Basera, Priyanka, Lavania, Meeta, Agnihotri, Anil, Lal, Banwari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400849/
https://www.ncbi.nlm.nih.gov/pubmed/30873133
http://dx.doi.org/10.3389/fmicb.2019.00282
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author Basera, Priyanka
Lavania, Meeta
Agnihotri, Anil
Lal, Banwari
author_facet Basera, Priyanka
Lavania, Meeta
Agnihotri, Anil
Lal, Banwari
author_sort Basera, Priyanka
collection PubMed
description Indian biodiversity is a hub for medicinal plants. Extensive research has been carried out to select plants with numerous properties which can be used for human welfare. Present research is about Cymbopogon citratus, an economically valuable medicinal plant. In this study Cymbopogon citratus was elected as a subject plant over the five selected plants (Azadirachta indica, Plumeria obtuse, Sapindus mukorossi, Capsicum annuum and Phyllanthus emblica) on the basis of antibacterial effect against dominating pathogenic species of gram positive (Bacillus cereus, Bacillus licheniformis) and gram negative (Pseudomonas aeruginosa, Escherichia coli) bacteria. Further, bioactive agents behind antibacterial potential of Cymbopogon citratus was analyzed using analytical method (Phyto-chemical, FTIR, NMR and GC-MS). Due to the broad antimicrobial spectrum, silver nanoparticles have turned into a noteworthy decision for the improvement of new medication. Therefore, this investigation further elaborated in the development of Cymbopogon citratus silver nano-particles (CNPs). Antibacterial potential of CNPs examine in a range of C(25)–C(150) (μg/ml) through minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) where, C(25) (μg/ml) concentration of CNPs were recorded as the MIC for all bacterial species and C(25) (μg/ml) and C(50) (μg/ml) noted as the MBC for Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus, Bacillus licheniformis, respectively. In agar disk diffusion assay of CNPs, maximum diameter of zone of inhibition was observed for C(150) (μg/ml) concentration Bacillus cereus (20.12 ± 0.42), Bacillus licheniformis (22.34 ± 0.4), Pseudomonas aeruginosa (35.23 ± 0.46) and Escherichia coli (31.87 ± 0.24). Involvement of bioactive component as a reducing and capping agent can be confirmed through FTIR spectrum of CNPs. Moreover XRD, EDXRF and SEM showed crystalline and cuboidal nature of CNPs with (∼)35 nm sizes. Prominently, cytotoxic analysis was conducted to understand the toxic effect of CNPs. This research highlights the potential of CNPs due to the bioactive components present in Cymbopogon citratus extract: Polyphenols (phenol; 1584.56 ± 16.32 mg/L, Flavanoids) and mixture of terpenoids (Citral, Myrcene, Farnesol, β-myrcene and β –Pinene)
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spelling pubmed-64008492019-03-14 Analytical Investigation of Cymbopogon citratus and Exploiting the Potential of Developed Silver Nanoparticle Against the Dominating Species of Pathogenic Bacteria Basera, Priyanka Lavania, Meeta Agnihotri, Anil Lal, Banwari Front Microbiol Microbiology Indian biodiversity is a hub for medicinal plants. Extensive research has been carried out to select plants with numerous properties which can be used for human welfare. Present research is about Cymbopogon citratus, an economically valuable medicinal plant. In this study Cymbopogon citratus was elected as a subject plant over the five selected plants (Azadirachta indica, Plumeria obtuse, Sapindus mukorossi, Capsicum annuum and Phyllanthus emblica) on the basis of antibacterial effect against dominating pathogenic species of gram positive (Bacillus cereus, Bacillus licheniformis) and gram negative (Pseudomonas aeruginosa, Escherichia coli) bacteria. Further, bioactive agents behind antibacterial potential of Cymbopogon citratus was analyzed using analytical method (Phyto-chemical, FTIR, NMR and GC-MS). Due to the broad antimicrobial spectrum, silver nanoparticles have turned into a noteworthy decision for the improvement of new medication. Therefore, this investigation further elaborated in the development of Cymbopogon citratus silver nano-particles (CNPs). Antibacterial potential of CNPs examine in a range of C(25)–C(150) (μg/ml) through minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) where, C(25) (μg/ml) concentration of CNPs were recorded as the MIC for all bacterial species and C(25) (μg/ml) and C(50) (μg/ml) noted as the MBC for Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus, Bacillus licheniformis, respectively. In agar disk diffusion assay of CNPs, maximum diameter of zone of inhibition was observed for C(150) (μg/ml) concentration Bacillus cereus (20.12 ± 0.42), Bacillus licheniformis (22.34 ± 0.4), Pseudomonas aeruginosa (35.23 ± 0.46) and Escherichia coli (31.87 ± 0.24). Involvement of bioactive component as a reducing and capping agent can be confirmed through FTIR spectrum of CNPs. Moreover XRD, EDXRF and SEM showed crystalline and cuboidal nature of CNPs with (∼)35 nm sizes. Prominently, cytotoxic analysis was conducted to understand the toxic effect of CNPs. This research highlights the potential of CNPs due to the bioactive components present in Cymbopogon citratus extract: Polyphenols (phenol; 1584.56 ± 16.32 mg/L, Flavanoids) and mixture of terpenoids (Citral, Myrcene, Farnesol, β-myrcene and β –Pinene) Frontiers Media S.A. 2019-02-27 /pmc/articles/PMC6400849/ /pubmed/30873133 http://dx.doi.org/10.3389/fmicb.2019.00282 Text en Copyright © 2019 Basera, Lavania, Agnihotri and Lal. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Basera, Priyanka
Lavania, Meeta
Agnihotri, Anil
Lal, Banwari
Analytical Investigation of Cymbopogon citratus and Exploiting the Potential of Developed Silver Nanoparticle Against the Dominating Species of Pathogenic Bacteria
title Analytical Investigation of Cymbopogon citratus and Exploiting the Potential of Developed Silver Nanoparticle Against the Dominating Species of Pathogenic Bacteria
title_full Analytical Investigation of Cymbopogon citratus and Exploiting the Potential of Developed Silver Nanoparticle Against the Dominating Species of Pathogenic Bacteria
title_fullStr Analytical Investigation of Cymbopogon citratus and Exploiting the Potential of Developed Silver Nanoparticle Against the Dominating Species of Pathogenic Bacteria
title_full_unstemmed Analytical Investigation of Cymbopogon citratus and Exploiting the Potential of Developed Silver Nanoparticle Against the Dominating Species of Pathogenic Bacteria
title_short Analytical Investigation of Cymbopogon citratus and Exploiting the Potential of Developed Silver Nanoparticle Against the Dominating Species of Pathogenic Bacteria
title_sort analytical investigation of cymbopogon citratus and exploiting the potential of developed silver nanoparticle against the dominating species of pathogenic bacteria
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400849/
https://www.ncbi.nlm.nih.gov/pubmed/30873133
http://dx.doi.org/10.3389/fmicb.2019.00282
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