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Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation

Dorso-ventral (DV) countershading is a highly-conserved pigmentary adaptation in vertebrates. In mammals, spatially regulated expression of agouti-signaling protein (ASIP) generates the difference in shading by driving a switch between the production of chemically-distinct melanins in melanocytes in...

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Autores principales: Cal, Laura, Suarez-Bregua, Paula, Comesaña, Pilar, Owen, Jennifer, Braasch, Ingo, Kelsh, Robert, Cerdá-Reverter, José Miguel, Rotllant, Josep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401153/
https://www.ncbi.nlm.nih.gov/pubmed/30837630
http://dx.doi.org/10.1038/s41598-019-40251-z
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author Cal, Laura
Suarez-Bregua, Paula
Comesaña, Pilar
Owen, Jennifer
Braasch, Ingo
Kelsh, Robert
Cerdá-Reverter, José Miguel
Rotllant, Josep
author_facet Cal, Laura
Suarez-Bregua, Paula
Comesaña, Pilar
Owen, Jennifer
Braasch, Ingo
Kelsh, Robert
Cerdá-Reverter, José Miguel
Rotllant, Josep
author_sort Cal, Laura
collection PubMed
description Dorso-ventral (DV) countershading is a highly-conserved pigmentary adaptation in vertebrates. In mammals, spatially regulated expression of agouti-signaling protein (ASIP) generates the difference in shading by driving a switch between the production of chemically-distinct melanins in melanocytes in dorsal and ventral regions. In contrast, fish countershading seemed to result from a patterned DV distribution of differently-coloured cell-types (chromatophores). Despite the cellular differences in the basis for counter-shading, previous observations suggested that Agouti signaling likely played a role in this patterning process in fish. To test the hypotheses that Agouti regulated counter-shading in fish, and that this depended upon spatial regulation of the numbers of each chromatophore type, we engineered asip1 homozygous knockout mutant zebrafish. We show that loss-of-function asip1 mutants lose DV countershading, and that this results from changed numbers of multiple pigment cell-types in the skin and on scales. Our findings identify asip1 as key in the establishment of DV countershading in fish, but show that the cellular mechanism for translating a conserved signaling gradient into a conserved pigmentary phenotype has been radically altered in the course of evolution.
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spelling pubmed-64011532019-03-07 Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation Cal, Laura Suarez-Bregua, Paula Comesaña, Pilar Owen, Jennifer Braasch, Ingo Kelsh, Robert Cerdá-Reverter, José Miguel Rotllant, Josep Sci Rep Article Dorso-ventral (DV) countershading is a highly-conserved pigmentary adaptation in vertebrates. In mammals, spatially regulated expression of agouti-signaling protein (ASIP) generates the difference in shading by driving a switch between the production of chemically-distinct melanins in melanocytes in dorsal and ventral regions. In contrast, fish countershading seemed to result from a patterned DV distribution of differently-coloured cell-types (chromatophores). Despite the cellular differences in the basis for counter-shading, previous observations suggested that Agouti signaling likely played a role in this patterning process in fish. To test the hypotheses that Agouti regulated counter-shading in fish, and that this depended upon spatial regulation of the numbers of each chromatophore type, we engineered asip1 homozygous knockout mutant zebrafish. We show that loss-of-function asip1 mutants lose DV countershading, and that this results from changed numbers of multiple pigment cell-types in the skin and on scales. Our findings identify asip1 as key in the establishment of DV countershading in fish, but show that the cellular mechanism for translating a conserved signaling gradient into a conserved pigmentary phenotype has been radically altered in the course of evolution. Nature Publishing Group UK 2019-03-05 /pmc/articles/PMC6401153/ /pubmed/30837630 http://dx.doi.org/10.1038/s41598-019-40251-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Cal, Laura
Suarez-Bregua, Paula
Comesaña, Pilar
Owen, Jennifer
Braasch, Ingo
Kelsh, Robert
Cerdá-Reverter, José Miguel
Rotllant, Josep
Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation
title Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation
title_full Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation
title_fullStr Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation
title_full_unstemmed Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation
title_short Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation
title_sort countershading in zebrafish results from an asip1 controlled dorsoventral gradient of pigment cell differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401153/
https://www.ncbi.nlm.nih.gov/pubmed/30837630
http://dx.doi.org/10.1038/s41598-019-40251-z
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