Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing

Broad use of CRISPR-Cas12a (formerly Cpf1) nucleases(1) has been hindered by the requirement for an extended TTTV protospacer adjacent motif (PAM)(2). To address this limitation, we engineered an enhanced Acidaminococcus sp. Cas12a variant (enAsCas12a) that has a substantially expanded targeting ran...

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Detalles Bibliográficos
Autores principales: Kleinstiver, Benjamin P., Sousa, Alexander A., Walton, Russell T., Tak, Y. Esther, Hsu, Jonathan Y., Clement, Kendell, Welch, Moira M., Horng, Joy E., Malagon-Lopez, Jose, Scarfò, Irene, Maus, Marcela V., Pinello, Luca, Aryee, Martin J., Joung, J. Keith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401248/
https://www.ncbi.nlm.nih.gov/pubmed/30742127
http://dx.doi.org/10.1038/s41587-018-0011-0

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