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Synthesis, Characterization and Cytotoxicity of Novel Thermoresponsive Star Copolymers of N,N′-Dimethylaminoethyl Methacrylate and Hydroxyl-Bearing Oligo(Ethylene Glycol) Methacrylate
Novel, nontoxic star copolymers of N,N-dimethylaminoethyl methacrylate (DMAEMA) and hydroxyl-bearing oligo(ethylene glycol) methacrylate (OEGMA-OH) were synthesized via atom transfer radical polymerization (ATRP) using hyperbranched poly(arylene oxindole) as the macroinitiator. Stars with molar mass...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401879/ https://www.ncbi.nlm.nih.gov/pubmed/30961179 http://dx.doi.org/10.3390/polym10111255 |
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author | Mendrek, Barbara Fus, Agnieszka Klarzyńska, Katarzyna Sieroń, Aleksander L. Smet, Mario Kowalczuk, Agnieszka Dworak, Andrzej |
author_facet | Mendrek, Barbara Fus, Agnieszka Klarzyńska, Katarzyna Sieroń, Aleksander L. Smet, Mario Kowalczuk, Agnieszka Dworak, Andrzej |
author_sort | Mendrek, Barbara |
collection | PubMed |
description | Novel, nontoxic star copolymers of N,N-dimethylaminoethyl methacrylate (DMAEMA) and hydroxyl-bearing oligo(ethylene glycol) methacrylate (OEGMA-OH) were synthesized via atom transfer radical polymerization (ATRP) using hyperbranched poly(arylene oxindole) as the macroinitiator. Stars with molar masses from 100,000 g/mol to 257,000 g/mol and with various amounts of OEGMA-OH in the arms were prepared. As these polymers can find applications, e.g., as carriers of nucleic acids, drugs or antibacterial or antifouling agents, in this work, much attention has been devoted to exploring their solution behavior and their stimuli-responsive properties. The behavior of the stars was studied in aqueous solutions under various pH and temperature conditions, as well as in PBS buffer, in Dulbecco’s modified Eagle’s medium (DMEM) and in organic solvents for comparison. The results indicated that increasing the content of hydrophilic OEGMA-OH units in the arms up to 10 mol% increased the cloud point temperature. For the stars with an OEGMA-OH content of 10 mol%, the thermo- and pH-responsivity was switched off. Since cytotoxicity experiments have shown that the obtained stars are less toxic than homopolymer DMAEMA stars, the presented studies confirmed that the prepared polymers are great candidates for the design of various nanosystems for biomedical applications. |
format | Online Article Text |
id | pubmed-6401879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64018792019-04-02 Synthesis, Characterization and Cytotoxicity of Novel Thermoresponsive Star Copolymers of N,N′-Dimethylaminoethyl Methacrylate and Hydroxyl-Bearing Oligo(Ethylene Glycol) Methacrylate Mendrek, Barbara Fus, Agnieszka Klarzyńska, Katarzyna Sieroń, Aleksander L. Smet, Mario Kowalczuk, Agnieszka Dworak, Andrzej Polymers (Basel) Article Novel, nontoxic star copolymers of N,N-dimethylaminoethyl methacrylate (DMAEMA) and hydroxyl-bearing oligo(ethylene glycol) methacrylate (OEGMA-OH) were synthesized via atom transfer radical polymerization (ATRP) using hyperbranched poly(arylene oxindole) as the macroinitiator. Stars with molar masses from 100,000 g/mol to 257,000 g/mol and with various amounts of OEGMA-OH in the arms were prepared. As these polymers can find applications, e.g., as carriers of nucleic acids, drugs or antibacterial or antifouling agents, in this work, much attention has been devoted to exploring their solution behavior and their stimuli-responsive properties. The behavior of the stars was studied in aqueous solutions under various pH and temperature conditions, as well as in PBS buffer, in Dulbecco’s modified Eagle’s medium (DMEM) and in organic solvents for comparison. The results indicated that increasing the content of hydrophilic OEGMA-OH units in the arms up to 10 mol% increased the cloud point temperature. For the stars with an OEGMA-OH content of 10 mol%, the thermo- and pH-responsivity was switched off. Since cytotoxicity experiments have shown that the obtained stars are less toxic than homopolymer DMAEMA stars, the presented studies confirmed that the prepared polymers are great candidates for the design of various nanosystems for biomedical applications. MDPI 2018-11-12 /pmc/articles/PMC6401879/ /pubmed/30961179 http://dx.doi.org/10.3390/polym10111255 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mendrek, Barbara Fus, Agnieszka Klarzyńska, Katarzyna Sieroń, Aleksander L. Smet, Mario Kowalczuk, Agnieszka Dworak, Andrzej Synthesis, Characterization and Cytotoxicity of Novel Thermoresponsive Star Copolymers of N,N′-Dimethylaminoethyl Methacrylate and Hydroxyl-Bearing Oligo(Ethylene Glycol) Methacrylate |
title | Synthesis, Characterization and Cytotoxicity of Novel Thermoresponsive Star Copolymers of N,N′-Dimethylaminoethyl Methacrylate and Hydroxyl-Bearing Oligo(Ethylene Glycol) Methacrylate |
title_full | Synthesis, Characterization and Cytotoxicity of Novel Thermoresponsive Star Copolymers of N,N′-Dimethylaminoethyl Methacrylate and Hydroxyl-Bearing Oligo(Ethylene Glycol) Methacrylate |
title_fullStr | Synthesis, Characterization and Cytotoxicity of Novel Thermoresponsive Star Copolymers of N,N′-Dimethylaminoethyl Methacrylate and Hydroxyl-Bearing Oligo(Ethylene Glycol) Methacrylate |
title_full_unstemmed | Synthesis, Characterization and Cytotoxicity of Novel Thermoresponsive Star Copolymers of N,N′-Dimethylaminoethyl Methacrylate and Hydroxyl-Bearing Oligo(Ethylene Glycol) Methacrylate |
title_short | Synthesis, Characterization and Cytotoxicity of Novel Thermoresponsive Star Copolymers of N,N′-Dimethylaminoethyl Methacrylate and Hydroxyl-Bearing Oligo(Ethylene Glycol) Methacrylate |
title_sort | synthesis, characterization and cytotoxicity of novel thermoresponsive star copolymers of n,n′-dimethylaminoethyl methacrylate and hydroxyl-bearing oligo(ethylene glycol) methacrylate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401879/ https://www.ncbi.nlm.nih.gov/pubmed/30961179 http://dx.doi.org/10.3390/polym10111255 |
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