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MALDI-TOF mass spectrometry: a new tool for rapid identification of cercariae (Trematoda, Digenea)
Identification of cercariae was long based on morphological and morphometric features, but these approaches remain difficult to implement and require skills that have now become rare. Molecular tools have become the reference even though they remain relatively time-consuming and expensive. We propos...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
EDP Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6402365/ https://www.ncbi.nlm.nih.gov/pubmed/30838972 http://dx.doi.org/10.1051/parasite/2019011 |
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author | Huguenin, Antoine Depaquit, Jérôme Villena, Isabelle Ferté, Hubert |
author_facet | Huguenin, Antoine Depaquit, Jérôme Villena, Isabelle Ferté, Hubert |
author_sort | Huguenin, Antoine |
collection | PubMed |
description | Identification of cercariae was long based on morphological and morphometric features, but these approaches remain difficult to implement and require skills that have now become rare. Molecular tools have become the reference even though they remain relatively time-consuming and expensive. We propose a new approach for the identification of cercariae using MALDI-TOF mass spectrometry. Snails of different genera (Radix, Lymnaea, Stagnicola, Planorbis, and Anisus) were collected in the field to perform emitting tests in the laboratory. The cercariae they emitted (Trichobilharzia anseri, Diplostomum pseudospathaceum, Alaria alata, Echinostoma revolutum, Petasiger phalacrocoracis, Tylodelphys sp., Australapatemon sp., Cotylurus sp., Posthodiplostomum sp., Parastrigea sp., Echinoparyphium sp. and Plagiorchis sp.) were characterized by sequencing the D2, ITS2 and ITS1 domains of rDNA, and by amplification using specific Alaria alata primers. A sample of each specimen, either fresh or stored in ethanol, was subjected to a simple preparation protocol for MALDI-TOF analysis. The main spectral profiles were analyzed by Hierarchical Clustering Analysis. Likewise, the haplotypes were analyzed using the maximum likelihood method. Analytical performance and the log-score value (LSV) cut-off for species identification were then assessed by blind testing. The clusters obtained by both techniques were congruent, allowing identification at a species level. MALDI-TOF enables identification at an LSV cut-off of 1.7 without false-positives; however, it requires more data on closely related species. The development of a “high throughput” identification system for all types of cercariae would be of considerable interest in epidemiological surveys of trematode infections. |
format | Online Article Text |
id | pubmed-6402365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-64023652019-03-29 MALDI-TOF mass spectrometry: a new tool for rapid identification of cercariae (Trematoda, Digenea) Huguenin, Antoine Depaquit, Jérôme Villena, Isabelle Ferté, Hubert Parasite Research Article Identification of cercariae was long based on morphological and morphometric features, but these approaches remain difficult to implement and require skills that have now become rare. Molecular tools have become the reference even though they remain relatively time-consuming and expensive. We propose a new approach for the identification of cercariae using MALDI-TOF mass spectrometry. Snails of different genera (Radix, Lymnaea, Stagnicola, Planorbis, and Anisus) were collected in the field to perform emitting tests in the laboratory. The cercariae they emitted (Trichobilharzia anseri, Diplostomum pseudospathaceum, Alaria alata, Echinostoma revolutum, Petasiger phalacrocoracis, Tylodelphys sp., Australapatemon sp., Cotylurus sp., Posthodiplostomum sp., Parastrigea sp., Echinoparyphium sp. and Plagiorchis sp.) were characterized by sequencing the D2, ITS2 and ITS1 domains of rDNA, and by amplification using specific Alaria alata primers. A sample of each specimen, either fresh or stored in ethanol, was subjected to a simple preparation protocol for MALDI-TOF analysis. The main spectral profiles were analyzed by Hierarchical Clustering Analysis. Likewise, the haplotypes were analyzed using the maximum likelihood method. Analytical performance and the log-score value (LSV) cut-off for species identification were then assessed by blind testing. The clusters obtained by both techniques were congruent, allowing identification at a species level. MALDI-TOF enables identification at an LSV cut-off of 1.7 without false-positives; however, it requires more data on closely related species. The development of a “high throughput” identification system for all types of cercariae would be of considerable interest in epidemiological surveys of trematode infections. EDP Sciences 2019-03-06 /pmc/articles/PMC6402365/ /pubmed/30838972 http://dx.doi.org/10.1051/parasite/2019011 Text en © A. Huguenin et al., published by EDP Sciences, 2019 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Huguenin, Antoine Depaquit, Jérôme Villena, Isabelle Ferté, Hubert MALDI-TOF mass spectrometry: a new tool for rapid identification of cercariae (Trematoda, Digenea) |
title | MALDI-TOF mass spectrometry: a new tool for rapid identification of cercariae (Trematoda, Digenea) |
title_full | MALDI-TOF mass spectrometry: a new tool for rapid identification of cercariae (Trematoda, Digenea) |
title_fullStr | MALDI-TOF mass spectrometry: a new tool for rapid identification of cercariae (Trematoda, Digenea) |
title_full_unstemmed | MALDI-TOF mass spectrometry: a new tool for rapid identification of cercariae (Trematoda, Digenea) |
title_short | MALDI-TOF mass spectrometry: a new tool for rapid identification of cercariae (Trematoda, Digenea) |
title_sort | maldi-tof mass spectrometry: a new tool for rapid identification of cercariae (trematoda, digenea) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6402365/ https://www.ncbi.nlm.nih.gov/pubmed/30838972 http://dx.doi.org/10.1051/parasite/2019011 |
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