Rotating magnetic field as tool for enhancing enzymes properties - laccase case study

The aim of this study was to analyse the effect of rotating magnetic field (RMF) exposition on the fungal laccase catalytic properties. The results obtained in the study revealed that RMF may positively alter the laccase activity. A significant increase in activities of 11%, 11%, and 9% were observe...

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Autores principales: Wasak, Agata, Drozd, Radosław, Jankowiak, Dorota, Rakoczy, Rafa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403337/
https://www.ncbi.nlm.nih.gov/pubmed/30842482
http://dx.doi.org/10.1038/s41598-019-39198-y
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author Wasak, Agata
Drozd, Radosław
Jankowiak, Dorota
Rakoczy, Rafa
author_facet Wasak, Agata
Drozd, Radosław
Jankowiak, Dorota
Rakoczy, Rafa
author_sort Wasak, Agata
collection PubMed
description The aim of this study was to analyse the effect of rotating magnetic field (RMF) exposition on the fungal laccase catalytic properties. The results obtained in the study revealed that RMF may positively alter the laccase activity. A significant increase in activities of 11%, 11%, and 9% were observed at 10 Hz, 40 Hz and 50 Hz, respectively. Exposure of laccase to the rotating magnetic field resulted in its increased activity at broader pH range and a slight shift in optimum pH from 4.0 to 4.5 at RMF with frequency 20 Hz. The results show that the enzyme activity, stability, and optimum pH can be significantly altered depending on the characteristic of the applied RMF. Application of rotating magnetic field opens a new way for controlling and directions of enzyme-based bioprocessing.
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spelling pubmed-64033372019-03-08 Rotating magnetic field as tool for enhancing enzymes properties - laccase case study Wasak, Agata Drozd, Radosław Jankowiak, Dorota Rakoczy, Rafa Sci Rep Article The aim of this study was to analyse the effect of rotating magnetic field (RMF) exposition on the fungal laccase catalytic properties. The results obtained in the study revealed that RMF may positively alter the laccase activity. A significant increase in activities of 11%, 11%, and 9% were observed at 10 Hz, 40 Hz and 50 Hz, respectively. Exposure of laccase to the rotating magnetic field resulted in its increased activity at broader pH range and a slight shift in optimum pH from 4.0 to 4.5 at RMF with frequency 20 Hz. The results show that the enzyme activity, stability, and optimum pH can be significantly altered depending on the characteristic of the applied RMF. Application of rotating magnetic field opens a new way for controlling and directions of enzyme-based bioprocessing. Nature Publishing Group UK 2019-03-06 /pmc/articles/PMC6403337/ /pubmed/30842482 http://dx.doi.org/10.1038/s41598-019-39198-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wasak, Agata
Drozd, Radosław
Jankowiak, Dorota
Rakoczy, Rafa
Rotating magnetic field as tool for enhancing enzymes properties - laccase case study
title Rotating magnetic field as tool for enhancing enzymes properties - laccase case study
title_full Rotating magnetic field as tool for enhancing enzymes properties - laccase case study
title_fullStr Rotating magnetic field as tool for enhancing enzymes properties - laccase case study
title_full_unstemmed Rotating magnetic field as tool for enhancing enzymes properties - laccase case study
title_short Rotating magnetic field as tool for enhancing enzymes properties - laccase case study
title_sort rotating magnetic field as tool for enhancing enzymes properties - laccase case study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403337/
https://www.ncbi.nlm.nih.gov/pubmed/30842482
http://dx.doi.org/10.1038/s41598-019-39198-y
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AT jankowiakdorota rotatingmagneticfieldastoolforenhancingenzymespropertieslaccasecasestudy
AT rakoczyrafa rotatingmagneticfieldastoolforenhancingenzymespropertieslaccasecasestudy

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