Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control

Cell-based studies of human ribonucleases traditionally rely on methods that deplete proteins slowly. We engineered cells in which the 3′→5′ exoribonucleases of the exosome complex, DIS3 and EXOSC10, can be rapidly eliminated to assess their immediate roles in nuclear RNA biology. The loss of DIS3 h...

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Detalles Bibliográficos
Autores principales: Davidson, Lee, Francis, Laura, Cordiner, Ross A., Eaton, Joshua D., Estell, Chris, Macias, Sara, Cáceres, Javier F., West, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403362/
https://www.ncbi.nlm.nih.gov/pubmed/30840897
http://dx.doi.org/10.1016/j.celrep.2019.02.012

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403362/
https://www.ncbi.nlm.nih.gov/pubmed/30840897
http://dx.doi.org/10.1016/j.celrep.2019.02.012

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