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Potential of 2,4-Dihydroxybenzoic Acid as an Oviposition Stimulant for Mass-Reared Ladybird Beetles

The discovery of inexpensive, readily available bioflavonoids, and their degradation products that boost the reproductive potential of mass-reared predators is the overarching goal of this research. We tested the hypothesis that 2,4-dihydroxybenzoic acid (DHBA), an inexpensive degradation product of...

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Autores principales: Riddick, E W, Wu, Z, Eller, F J, Berhow, M A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403478/
https://www.ncbi.nlm.nih.gov/pubmed/30822780
http://dx.doi.org/10.1093/jisesa/iez012
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author Riddick, E W
Wu, Z
Eller, F J
Berhow, M A
author_facet Riddick, E W
Wu, Z
Eller, F J
Berhow, M A
author_sort Riddick, E W
collection PubMed
description The discovery of inexpensive, readily available bioflavonoids, and their degradation products that boost the reproductive potential of mass-reared predators is the overarching goal of this research. We tested the hypothesis that 2,4-dihydroxybenzoic acid (DHBA), an inexpensive degradation product of morin (a flavonol bioflavonoid), stimulates oviposition by the ladybird beetle Coleomegilla maculata (DeGeer). We also tested the hypothesis that C. maculata females must touch or taste DHBA to stimulate oviposition. We setup bioassays in communal cages (housing 10 females) and solitary cages (housing 1 female). In communal cages, nearly all egg clutches were found in or near the chemical dish with DHBA only. Provisioning cages with a tissue substrate reduced oviposition in the chemical dish. Regardless of oviposition site, egg number per clutch did not increase in communal cages or solitary cages with DHBA only. Affixing DHBA to the base of the chemical dish, then covering it with a nylon screen, reduced oviposition. This study suggests that females must touch or taste DHBA to stimulate oviposition. The physiological mechanism involved in oviposition stimulation requires further study. DHBA could potentially serve as a weak oviposition stimulant for predatory ladybird beetles in some mass-rearing systems.
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spelling pubmed-64034782019-03-12 Potential of 2,4-Dihydroxybenzoic Acid as an Oviposition Stimulant for Mass-Reared Ladybird Beetles Riddick, E W Wu, Z Eller, F J Berhow, M A J Insect Sci Research Article The discovery of inexpensive, readily available bioflavonoids, and their degradation products that boost the reproductive potential of mass-reared predators is the overarching goal of this research. We tested the hypothesis that 2,4-dihydroxybenzoic acid (DHBA), an inexpensive degradation product of morin (a flavonol bioflavonoid), stimulates oviposition by the ladybird beetle Coleomegilla maculata (DeGeer). We also tested the hypothesis that C. maculata females must touch or taste DHBA to stimulate oviposition. We setup bioassays in communal cages (housing 10 females) and solitary cages (housing 1 female). In communal cages, nearly all egg clutches were found in or near the chemical dish with DHBA only. Provisioning cages with a tissue substrate reduced oviposition in the chemical dish. Regardless of oviposition site, egg number per clutch did not increase in communal cages or solitary cages with DHBA only. Affixing DHBA to the base of the chemical dish, then covering it with a nylon screen, reduced oviposition. This study suggests that females must touch or taste DHBA to stimulate oviposition. The physiological mechanism involved in oviposition stimulation requires further study. DHBA could potentially serve as a weak oviposition stimulant for predatory ladybird beetles in some mass-rearing systems. Oxford University Press 2019-03-07 /pmc/articles/PMC6403478/ /pubmed/30822780 http://dx.doi.org/10.1093/jisesa/iez012 Text en Published by Oxford University Press on behalf of Entomological Society of America 2019. This work is written by (a) US Government employee(s) and is in the public domain in the US. http://creativecommons.org/licenses//4.0/ This Open Access article contains public sector information licensed under the Open Government Licence v2.0 (http://www.nationalarchives.gov.uk/doc/open-government-licence/version/2/).
spellingShingle Research Article
Riddick, E W
Wu, Z
Eller, F J
Berhow, M A
Potential of 2,4-Dihydroxybenzoic Acid as an Oviposition Stimulant for Mass-Reared Ladybird Beetles
title Potential of 2,4-Dihydroxybenzoic Acid as an Oviposition Stimulant for Mass-Reared Ladybird Beetles
title_full Potential of 2,4-Dihydroxybenzoic Acid as an Oviposition Stimulant for Mass-Reared Ladybird Beetles
title_fullStr Potential of 2,4-Dihydroxybenzoic Acid as an Oviposition Stimulant for Mass-Reared Ladybird Beetles
title_full_unstemmed Potential of 2,4-Dihydroxybenzoic Acid as an Oviposition Stimulant for Mass-Reared Ladybird Beetles
title_short Potential of 2,4-Dihydroxybenzoic Acid as an Oviposition Stimulant for Mass-Reared Ladybird Beetles
title_sort potential of 2,4-dihydroxybenzoic acid as an oviposition stimulant for mass-reared ladybird beetles
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403478/
https://www.ncbi.nlm.nih.gov/pubmed/30822780
http://dx.doi.org/10.1093/jisesa/iez012
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