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Biomass Extraction Using Non-Chlorinated Solvents for Biocompatibility Improvement of Polyhydroxyalkanoates

An economically viable method to extract polyhydroxyalkanoates (PHAs) from cells is desirable for this biodegradable polymer of potential biomedical applications. In this work, two non-chlorinated solvents, cyclohexanone and γ-butyrolactone, were examined for extracting PHA produced by the bacterial...

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Autores principales: Jiang, Guozhan, Johnston, Brian, Townrow, David E., Radecka, Iza, Koller, Martin, Chaber, Paweł, Adamus, Grażyna, Kowalczuk, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403533/
https://www.ncbi.nlm.nih.gov/pubmed/30960656
http://dx.doi.org/10.3390/polym10070731
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author Jiang, Guozhan
Johnston, Brian
Townrow, David E.
Radecka, Iza
Koller, Martin
Chaber, Paweł
Adamus, Grażyna
Kowalczuk, Marek
author_facet Jiang, Guozhan
Johnston, Brian
Townrow, David E.
Radecka, Iza
Koller, Martin
Chaber, Paweł
Adamus, Grażyna
Kowalczuk, Marek
author_sort Jiang, Guozhan
collection PubMed
description An economically viable method to extract polyhydroxyalkanoates (PHAs) from cells is desirable for this biodegradable polymer of potential biomedical applications. In this work, two non-chlorinated solvents, cyclohexanone and γ-butyrolactone, were examined for extracting PHA produced by the bacterial strain Cupriavidus necator H16 cultivated on vegetable oil as a sole carbon source. The PHA produced was determined as a poly(3-hydroxybutyrate) (PHB) homopolyester. The extraction kinetics of the two solvents was determined using gel permeation chromatography (GPC). When cyclohexanone was used as the extraction solvent at 120 °C in 3 min, 95% of the PHB was recovered from the cells with a similar purity to that extracted using chloroform. With a decrease in temperature, the recovery yield decreased. At the same temperatures, the recovery yield of γ-butyrolactone was significantly lower. The effect of the two solvents on the quality of the extracted PHB was also examined using GPC and elemental analysis. The molar mass and dispersity of the obtained polymer were similar to that extracted using chloroform, while the nitrogen content of the PHB extracted using the two new solvents was slightly higher. In a nutshell, cyclohexanone in particular was identified as an expedient candidate to efficiently drive novel, sustainable PHA extraction processes.
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spelling pubmed-64035332019-04-02 Biomass Extraction Using Non-Chlorinated Solvents for Biocompatibility Improvement of Polyhydroxyalkanoates Jiang, Guozhan Johnston, Brian Townrow, David E. Radecka, Iza Koller, Martin Chaber, Paweł Adamus, Grażyna Kowalczuk, Marek Polymers (Basel) Article An economically viable method to extract polyhydroxyalkanoates (PHAs) from cells is desirable for this biodegradable polymer of potential biomedical applications. In this work, two non-chlorinated solvents, cyclohexanone and γ-butyrolactone, were examined for extracting PHA produced by the bacterial strain Cupriavidus necator H16 cultivated on vegetable oil as a sole carbon source. The PHA produced was determined as a poly(3-hydroxybutyrate) (PHB) homopolyester. The extraction kinetics of the two solvents was determined using gel permeation chromatography (GPC). When cyclohexanone was used as the extraction solvent at 120 °C in 3 min, 95% of the PHB was recovered from the cells with a similar purity to that extracted using chloroform. With a decrease in temperature, the recovery yield decreased. At the same temperatures, the recovery yield of γ-butyrolactone was significantly lower. The effect of the two solvents on the quality of the extracted PHB was also examined using GPC and elemental analysis. The molar mass and dispersity of the obtained polymer were similar to that extracted using chloroform, while the nitrogen content of the PHB extracted using the two new solvents was slightly higher. In a nutshell, cyclohexanone in particular was identified as an expedient candidate to efficiently drive novel, sustainable PHA extraction processes. MDPI 2018-07-03 /pmc/articles/PMC6403533/ /pubmed/30960656 http://dx.doi.org/10.3390/polym10070731 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jiang, Guozhan
Johnston, Brian
Townrow, David E.
Radecka, Iza
Koller, Martin
Chaber, Paweł
Adamus, Grażyna
Kowalczuk, Marek
Biomass Extraction Using Non-Chlorinated Solvents for Biocompatibility Improvement of Polyhydroxyalkanoates
title Biomass Extraction Using Non-Chlorinated Solvents for Biocompatibility Improvement of Polyhydroxyalkanoates
title_full Biomass Extraction Using Non-Chlorinated Solvents for Biocompatibility Improvement of Polyhydroxyalkanoates
title_fullStr Biomass Extraction Using Non-Chlorinated Solvents for Biocompatibility Improvement of Polyhydroxyalkanoates
title_full_unstemmed Biomass Extraction Using Non-Chlorinated Solvents for Biocompatibility Improvement of Polyhydroxyalkanoates
title_short Biomass Extraction Using Non-Chlorinated Solvents for Biocompatibility Improvement of Polyhydroxyalkanoates
title_sort biomass extraction using non-chlorinated solvents for biocompatibility improvement of polyhydroxyalkanoates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403533/
https://www.ncbi.nlm.nih.gov/pubmed/30960656
http://dx.doi.org/10.3390/polym10070731
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