Cargando…
Evaluation of the Bactericidal and Fungicidal Activities of Poly([2-(methacryloyloxy)ethyl]trimethyl Ammonium Chloride)(Poly (METAC))-Based Materials
Poly([2-(methacryloyloxy)ethyl]trimethyl ammonium chloride) (METAC) and the gels were prepared and evaluated for their bactericidal and fungicidal activities. The antimicrobial properties of poly(METAC) were tested against Escherichia coli (E. coli), Bacillus subtilis (B. subtilis), Saccharomyces ce...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403553/ https://www.ncbi.nlm.nih.gov/pubmed/30960872 http://dx.doi.org/10.3390/polym10090947 |
_version_ | 1783400635016675328 |
---|---|
author | Shiga, Toshiki Mori, Hiromitsu Uemura, Keiichi Moriuchi, Ryota Dohra, Hideo Yamawaki-Ogata, Aika Narita, Yuji Saito, Akihiro Kotsuchibashi, Yohei |
author_facet | Shiga, Toshiki Mori, Hiromitsu Uemura, Keiichi Moriuchi, Ryota Dohra, Hideo Yamawaki-Ogata, Aika Narita, Yuji Saito, Akihiro Kotsuchibashi, Yohei |
author_sort | Shiga, Toshiki |
collection | PubMed |
description | Poly([2-(methacryloyloxy)ethyl]trimethyl ammonium chloride) (METAC) and the gels were prepared and evaluated for their bactericidal and fungicidal activities. The antimicrobial properties of poly(METAC) were tested against Escherichia coli (E. coli), Bacillus subtilis (B. subtilis), Saccharomyces cerevisiae (Sa. cerevisiae), methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), and Candida albicans (C. albicans). Moreover, the structural forms of the linear and cross-linked poly(METAC) were investigated for their influences on bacterial aggregation, precipitation, and cell-death. To our knowledge, this is the first report on the comparison of the antimicrobial properties of poly(METAC) and poly(METAC)-gels. The bactericidal and fungicidal activities were evaluated by determining minimum inhibitory concentrations (MICs), UV–Vis spectroscopy, and fluorescence and confocal microscopies. The MICs were found to be 123 (MSSA), 123 (MRSA), 123 (P. aeruginosa), 370 (E. coli), 123 (B. subtilis), 370 (C. albicans), and 370 μg/mL (Sa. cerevisiae), as determined by broth dilution, and 370 (MSSA), 370 (MRSA), 370 (P. aeruginosa), 3300 (E. coli), 370 (B. subtilis), 1100 (C. albicans), and >10,000 μg/mL (Sa. cerevisiae), as determined by paper disc diffusion (on solid medium). The poly(METAC)-gels achieved rapid adsorption/precipitation of bacteria via the cationic surface charge. Thus, these poly(METAC)-based polymers can potentially be used as antibacterial materials. |
format | Online Article Text |
id | pubmed-6403553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64035532019-04-02 Evaluation of the Bactericidal and Fungicidal Activities of Poly([2-(methacryloyloxy)ethyl]trimethyl Ammonium Chloride)(Poly (METAC))-Based Materials Shiga, Toshiki Mori, Hiromitsu Uemura, Keiichi Moriuchi, Ryota Dohra, Hideo Yamawaki-Ogata, Aika Narita, Yuji Saito, Akihiro Kotsuchibashi, Yohei Polymers (Basel) Article Poly([2-(methacryloyloxy)ethyl]trimethyl ammonium chloride) (METAC) and the gels were prepared and evaluated for their bactericidal and fungicidal activities. The antimicrobial properties of poly(METAC) were tested against Escherichia coli (E. coli), Bacillus subtilis (B. subtilis), Saccharomyces cerevisiae (Sa. cerevisiae), methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), and Candida albicans (C. albicans). Moreover, the structural forms of the linear and cross-linked poly(METAC) were investigated for their influences on bacterial aggregation, precipitation, and cell-death. To our knowledge, this is the first report on the comparison of the antimicrobial properties of poly(METAC) and poly(METAC)-gels. The bactericidal and fungicidal activities were evaluated by determining minimum inhibitory concentrations (MICs), UV–Vis spectroscopy, and fluorescence and confocal microscopies. The MICs were found to be 123 (MSSA), 123 (MRSA), 123 (P. aeruginosa), 370 (E. coli), 123 (B. subtilis), 370 (C. albicans), and 370 μg/mL (Sa. cerevisiae), as determined by broth dilution, and 370 (MSSA), 370 (MRSA), 370 (P. aeruginosa), 3300 (E. coli), 370 (B. subtilis), 1100 (C. albicans), and >10,000 μg/mL (Sa. cerevisiae), as determined by paper disc diffusion (on solid medium). The poly(METAC)-gels achieved rapid adsorption/precipitation of bacteria via the cationic surface charge. Thus, these poly(METAC)-based polymers can potentially be used as antibacterial materials. MDPI 2018-08-26 /pmc/articles/PMC6403553/ /pubmed/30960872 http://dx.doi.org/10.3390/polym10090947 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shiga, Toshiki Mori, Hiromitsu Uemura, Keiichi Moriuchi, Ryota Dohra, Hideo Yamawaki-Ogata, Aika Narita, Yuji Saito, Akihiro Kotsuchibashi, Yohei Evaluation of the Bactericidal and Fungicidal Activities of Poly([2-(methacryloyloxy)ethyl]trimethyl Ammonium Chloride)(Poly (METAC))-Based Materials |
title | Evaluation of the Bactericidal and Fungicidal Activities of Poly([2-(methacryloyloxy)ethyl]trimethyl Ammonium Chloride)(Poly (METAC))-Based Materials |
title_full | Evaluation of the Bactericidal and Fungicidal Activities of Poly([2-(methacryloyloxy)ethyl]trimethyl Ammonium Chloride)(Poly (METAC))-Based Materials |
title_fullStr | Evaluation of the Bactericidal and Fungicidal Activities of Poly([2-(methacryloyloxy)ethyl]trimethyl Ammonium Chloride)(Poly (METAC))-Based Materials |
title_full_unstemmed | Evaluation of the Bactericidal and Fungicidal Activities of Poly([2-(methacryloyloxy)ethyl]trimethyl Ammonium Chloride)(Poly (METAC))-Based Materials |
title_short | Evaluation of the Bactericidal and Fungicidal Activities of Poly([2-(methacryloyloxy)ethyl]trimethyl Ammonium Chloride)(Poly (METAC))-Based Materials |
title_sort | evaluation of the bactericidal and fungicidal activities of poly([2-(methacryloyloxy)ethyl]trimethyl ammonium chloride)(poly (metac))-based materials |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403553/ https://www.ncbi.nlm.nih.gov/pubmed/30960872 http://dx.doi.org/10.3390/polym10090947 |
work_keys_str_mv | AT shigatoshiki evaluationofthebactericidalandfungicidalactivitiesofpoly2methacryloyloxyethyltrimethylammoniumchloridepolymetacbasedmaterials AT morihiromitsu evaluationofthebactericidalandfungicidalactivitiesofpoly2methacryloyloxyethyltrimethylammoniumchloridepolymetacbasedmaterials AT uemurakeiichi evaluationofthebactericidalandfungicidalactivitiesofpoly2methacryloyloxyethyltrimethylammoniumchloridepolymetacbasedmaterials AT moriuchiryota evaluationofthebactericidalandfungicidalactivitiesofpoly2methacryloyloxyethyltrimethylammoniumchloridepolymetacbasedmaterials AT dohrahideo evaluationofthebactericidalandfungicidalactivitiesofpoly2methacryloyloxyethyltrimethylammoniumchloridepolymetacbasedmaterials AT yamawakiogataaika evaluationofthebactericidalandfungicidalactivitiesofpoly2methacryloyloxyethyltrimethylammoniumchloridepolymetacbasedmaterials AT naritayuji evaluationofthebactericidalandfungicidalactivitiesofpoly2methacryloyloxyethyltrimethylammoniumchloridepolymetacbasedmaterials AT saitoakihiro evaluationofthebactericidalandfungicidalactivitiesofpoly2methacryloyloxyethyltrimethylammoniumchloridepolymetacbasedmaterials AT kotsuchibashiyohei evaluationofthebactericidalandfungicidalactivitiesofpoly2methacryloyloxyethyltrimethylammoniumchloridepolymetacbasedmaterials |