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Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers

Molecularly imprinted polymers (MIPs) have proven to be particularly effective chemical probes for the molecular recognition of proteins, DNA, and viruses. Here, we started from a filamentous bacteriophage to synthesize a multi-functionalized MIP for detecting the acidic pharmaceutic clofibric acid...

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Detalles Bibliográficos
Autores principales: Baek, In-Hyuk, Han, Hyung-Seop, Baik, Seungyun, Helms, Volkhard, Kim, Youngjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403656/
https://www.ncbi.nlm.nih.gov/pubmed/30960899
http://dx.doi.org/10.3390/polym10090974
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author Baek, In-Hyuk
Han, Hyung-Seop
Baik, Seungyun
Helms, Volkhard
Kim, Youngjun
author_facet Baek, In-Hyuk
Han, Hyung-Seop
Baik, Seungyun
Helms, Volkhard
Kim, Youngjun
author_sort Baek, In-Hyuk
collection PubMed
description Molecularly imprinted polymers (MIPs) have proven to be particularly effective chemical probes for the molecular recognition of proteins, DNA, and viruses. Here, we started from a filamentous bacteriophage to synthesize a multi-functionalized MIP for detecting the acidic pharmaceutic clofibric acid (CA) as a chemical pollutant. Adsorption and quartz crystal microbalance with dissipation monitoring experiments showed that the phage-functionalized MIP had a good binding affinity for CA, compared with the non-imprinted polymer and MIP. In addition, the reusability of the phage-functionalized MIP was demonstrated for at least five repeated cycles, without significant loss in the binding activity. The results indicate that the exposed amino acids of the phage, together with the polymer matrix, create functional binding cavities that provide higher affinity to acidic pharmaceutical compounds.
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spelling pubmed-64036562019-04-02 Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers Baek, In-Hyuk Han, Hyung-Seop Baik, Seungyun Helms, Volkhard Kim, Youngjun Polymers (Basel) Article Molecularly imprinted polymers (MIPs) have proven to be particularly effective chemical probes for the molecular recognition of proteins, DNA, and viruses. Here, we started from a filamentous bacteriophage to synthesize a multi-functionalized MIP for detecting the acidic pharmaceutic clofibric acid (CA) as a chemical pollutant. Adsorption and quartz crystal microbalance with dissipation monitoring experiments showed that the phage-functionalized MIP had a good binding affinity for CA, compared with the non-imprinted polymer and MIP. In addition, the reusability of the phage-functionalized MIP was demonstrated for at least five repeated cycles, without significant loss in the binding activity. The results indicate that the exposed amino acids of the phage, together with the polymer matrix, create functional binding cavities that provide higher affinity to acidic pharmaceutical compounds. MDPI 2018-09-01 /pmc/articles/PMC6403656/ /pubmed/30960899 http://dx.doi.org/10.3390/polym10090974 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baek, In-Hyuk
Han, Hyung-Seop
Baik, Seungyun
Helms, Volkhard
Kim, Youngjun
Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers
title Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers
title_full Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers
title_fullStr Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers
title_full_unstemmed Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers
title_short Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers
title_sort detection of acidic pharmaceutical compounds using virus-based molecularly imprinted polymers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403656/
https://www.ncbi.nlm.nih.gov/pubmed/30960899
http://dx.doi.org/10.3390/polym10090974
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