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Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers
Molecularly imprinted polymers (MIPs) have proven to be particularly effective chemical probes for the molecular recognition of proteins, DNA, and viruses. Here, we started from a filamentous bacteriophage to synthesize a multi-functionalized MIP for detecting the acidic pharmaceutic clofibric acid...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403656/ https://www.ncbi.nlm.nih.gov/pubmed/30960899 http://dx.doi.org/10.3390/polym10090974 |
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author | Baek, In-Hyuk Han, Hyung-Seop Baik, Seungyun Helms, Volkhard Kim, Youngjun |
author_facet | Baek, In-Hyuk Han, Hyung-Seop Baik, Seungyun Helms, Volkhard Kim, Youngjun |
author_sort | Baek, In-Hyuk |
collection | PubMed |
description | Molecularly imprinted polymers (MIPs) have proven to be particularly effective chemical probes for the molecular recognition of proteins, DNA, and viruses. Here, we started from a filamentous bacteriophage to synthesize a multi-functionalized MIP for detecting the acidic pharmaceutic clofibric acid (CA) as a chemical pollutant. Adsorption and quartz crystal microbalance with dissipation monitoring experiments showed that the phage-functionalized MIP had a good binding affinity for CA, compared with the non-imprinted polymer and MIP. In addition, the reusability of the phage-functionalized MIP was demonstrated for at least five repeated cycles, without significant loss in the binding activity. The results indicate that the exposed amino acids of the phage, together with the polymer matrix, create functional binding cavities that provide higher affinity to acidic pharmaceutical compounds. |
format | Online Article Text |
id | pubmed-6403656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64036562019-04-02 Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers Baek, In-Hyuk Han, Hyung-Seop Baik, Seungyun Helms, Volkhard Kim, Youngjun Polymers (Basel) Article Molecularly imprinted polymers (MIPs) have proven to be particularly effective chemical probes for the molecular recognition of proteins, DNA, and viruses. Here, we started from a filamentous bacteriophage to synthesize a multi-functionalized MIP for detecting the acidic pharmaceutic clofibric acid (CA) as a chemical pollutant. Adsorption and quartz crystal microbalance with dissipation monitoring experiments showed that the phage-functionalized MIP had a good binding affinity for CA, compared with the non-imprinted polymer and MIP. In addition, the reusability of the phage-functionalized MIP was demonstrated for at least five repeated cycles, without significant loss in the binding activity. The results indicate that the exposed amino acids of the phage, together with the polymer matrix, create functional binding cavities that provide higher affinity to acidic pharmaceutical compounds. MDPI 2018-09-01 /pmc/articles/PMC6403656/ /pubmed/30960899 http://dx.doi.org/10.3390/polym10090974 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Baek, In-Hyuk Han, Hyung-Seop Baik, Seungyun Helms, Volkhard Kim, Youngjun Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers |
title | Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers |
title_full | Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers |
title_fullStr | Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers |
title_full_unstemmed | Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers |
title_short | Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers |
title_sort | detection of acidic pharmaceutical compounds using virus-based molecularly imprinted polymers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403656/ https://www.ncbi.nlm.nih.gov/pubmed/30960899 http://dx.doi.org/10.3390/polym10090974 |
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