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A Cellulose/Laponite Interpenetrated Polymer Network (IPN) Hydrogel: Controllable Double-Network Structure with High Modulus

Laponite XLS™, which is a synthetic clay of nanometric dimensions containing a peptizing agent, has been associated with silanized hydroxypropylmethylcellulose (Si-HPMC) to form, after crosslinking, a novel composite hydrogel. Different protocols of sample preparation were used, leading to different...

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Autores principales: Xie, Fan, Boyer, Cécile, Gaborit, Victor, Rouillon, Thierry, Guicheux, Jérôme, Tassin, Jean-François, Geoffroy, Valérie, Réthoré, Gildas, Weiss, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403786/
https://www.ncbi.nlm.nih.gov/pubmed/30966668
http://dx.doi.org/10.3390/polym10060634
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author Xie, Fan
Boyer, Cécile
Gaborit, Victor
Rouillon, Thierry
Guicheux, Jérôme
Tassin, Jean-François
Geoffroy, Valérie
Réthoré, Gildas
Weiss, Pierre
author_facet Xie, Fan
Boyer, Cécile
Gaborit, Victor
Rouillon, Thierry
Guicheux, Jérôme
Tassin, Jean-François
Geoffroy, Valérie
Réthoré, Gildas
Weiss, Pierre
author_sort Xie, Fan
collection PubMed
description Laponite XLS™, which is a synthetic clay of nanometric dimensions containing a peptizing agent, has been associated with silanized hydroxypropylmethylcellulose (Si-HPMC) to form, after crosslinking, a novel composite hydrogel. Different protocols of sample preparation were used, leading to different morphologies. A key result was that the storage modulus of Si-HPMC/XLS composite hydrogel could be increased ten times when compared to that of pure Si-HPMC hydrogel using 2 wt % of Laponite. The viscoelastic properties of the composite formulations indicated that chemical and physical network structures co-existed in the Si-HPMC/XLS composite hydrogel. Images that were obtained from confocal laser scanning microscopy using labelled Laponite XLS in the composite hydrogels show two co-continuous areas: red light area and dark area. The tracking of fluorescent microspheres motions in the composite formulations revealed that the red-light area was a dense structure, whereas the dark area was rather loose without aggregated Laponite. This novel special double-network structure facilitates the composite hydrogel to be an adapted biomaterial for specific tissue engineering. Unfortunately, cytotoxicity’s assays suggested that XLS Laponites are cytotoxic at low concentration. This study validates that the hybrid interpenetrated network IPN hydrogel has a high modulus that has adapted for tissue engineering, but the cell’s internalization of Laponites has to be controlled.
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spelling pubmed-64037862019-04-02 A Cellulose/Laponite Interpenetrated Polymer Network (IPN) Hydrogel: Controllable Double-Network Structure with High Modulus Xie, Fan Boyer, Cécile Gaborit, Victor Rouillon, Thierry Guicheux, Jérôme Tassin, Jean-François Geoffroy, Valérie Réthoré, Gildas Weiss, Pierre Polymers (Basel) Article Laponite XLS™, which is a synthetic clay of nanometric dimensions containing a peptizing agent, has been associated with silanized hydroxypropylmethylcellulose (Si-HPMC) to form, after crosslinking, a novel composite hydrogel. Different protocols of sample preparation were used, leading to different morphologies. A key result was that the storage modulus of Si-HPMC/XLS composite hydrogel could be increased ten times when compared to that of pure Si-HPMC hydrogel using 2 wt % of Laponite. The viscoelastic properties of the composite formulations indicated that chemical and physical network structures co-existed in the Si-HPMC/XLS composite hydrogel. Images that were obtained from confocal laser scanning microscopy using labelled Laponite XLS in the composite hydrogels show two co-continuous areas: red light area and dark area. The tracking of fluorescent microspheres motions in the composite formulations revealed that the red-light area was a dense structure, whereas the dark area was rather loose without aggregated Laponite. This novel special double-network structure facilitates the composite hydrogel to be an adapted biomaterial for specific tissue engineering. Unfortunately, cytotoxicity’s assays suggested that XLS Laponites are cytotoxic at low concentration. This study validates that the hybrid interpenetrated network IPN hydrogel has a high modulus that has adapted for tissue engineering, but the cell’s internalization of Laponites has to be controlled. MDPI 2018-06-08 /pmc/articles/PMC6403786/ /pubmed/30966668 http://dx.doi.org/10.3390/polym10060634 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xie, Fan
Boyer, Cécile
Gaborit, Victor
Rouillon, Thierry
Guicheux, Jérôme
Tassin, Jean-François
Geoffroy, Valérie
Réthoré, Gildas
Weiss, Pierre
A Cellulose/Laponite Interpenetrated Polymer Network (IPN) Hydrogel: Controllable Double-Network Structure with High Modulus
title A Cellulose/Laponite Interpenetrated Polymer Network (IPN) Hydrogel: Controllable Double-Network Structure with High Modulus
title_full A Cellulose/Laponite Interpenetrated Polymer Network (IPN) Hydrogel: Controllable Double-Network Structure with High Modulus
title_fullStr A Cellulose/Laponite Interpenetrated Polymer Network (IPN) Hydrogel: Controllable Double-Network Structure with High Modulus
title_full_unstemmed A Cellulose/Laponite Interpenetrated Polymer Network (IPN) Hydrogel: Controllable Double-Network Structure with High Modulus
title_short A Cellulose/Laponite Interpenetrated Polymer Network (IPN) Hydrogel: Controllable Double-Network Structure with High Modulus
title_sort cellulose/laponite interpenetrated polymer network (ipn) hydrogel: controllable double-network structure with high modulus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403786/
https://www.ncbi.nlm.nih.gov/pubmed/30966668
http://dx.doi.org/10.3390/polym10060634
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