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Extraction of Keratin from Rabbit Hair by a Deep Eutectic Solvent and Its Characterization
Keratin from a variety of sources is one of the most abundant biopolymers. In livestock and textile industries, a large amount of rabbit hair waste is produced every year, and therefore it is of great significance to extract keratin from waste rabbit hair in terms of the treatment and utilization of...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404039/ https://www.ncbi.nlm.nih.gov/pubmed/30960918 http://dx.doi.org/10.3390/polym10090993 |
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author | Wang, Dongyue Yang, Xu-Hong Tang, Ren-Cheng Yao, Fan |
author_facet | Wang, Dongyue Yang, Xu-Hong Tang, Ren-Cheng Yao, Fan |
author_sort | Wang, Dongyue |
collection | PubMed |
description | Keratin from a variety of sources is one of the most abundant biopolymers. In livestock and textile industries, a large amount of rabbit hair waste is produced every year, and therefore it is of great significance to extract keratin from waste rabbit hair in terms of the treatment and utilization of wastes. In this study, a novel, eco-friendly and benign choline chloride/oxalic acid deep eutectic solvent at a molar ratio of 1:2 was applied to dissolve waste rabbit hair, and after dissolution keratin was separated by dialysis, filtration, and freeze-drying. The dissolution temperature effect was discussed, and the resulting keratin powder was characterized by X-ray diffraction, scanning electron microscope, Fourier transform infrared spectroscopy, protein electrophoresis, thermogravimetry and differential scanning calorimetry, and amino acid analysis. During the dissolution process, the α-helix structure of rabbit hair was deconstructed, and the disulfide bond linkages were broken. The solubility of rabbit hair was significantly enhanced by increasing dissolution temperature, and reached 88% at 120 °C. The keratin produced by dissolving at 120 °C displayed flaky powders after freeze-drying, and had a molecular weight ranging from 3.8 to 5.8 kDa with a high proportion of serine, glutamic acid, cysteine, leucine, and arginine. Such features of molecular weight and amino acid distribution provide more choices for the diverse applications of keratin materials. |
format | Online Article Text |
id | pubmed-6404039 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64040392019-04-02 Extraction of Keratin from Rabbit Hair by a Deep Eutectic Solvent and Its Characterization Wang, Dongyue Yang, Xu-Hong Tang, Ren-Cheng Yao, Fan Polymers (Basel) Article Keratin from a variety of sources is one of the most abundant biopolymers. In livestock and textile industries, a large amount of rabbit hair waste is produced every year, and therefore it is of great significance to extract keratin from waste rabbit hair in terms of the treatment and utilization of wastes. In this study, a novel, eco-friendly and benign choline chloride/oxalic acid deep eutectic solvent at a molar ratio of 1:2 was applied to dissolve waste rabbit hair, and after dissolution keratin was separated by dialysis, filtration, and freeze-drying. The dissolution temperature effect was discussed, and the resulting keratin powder was characterized by X-ray diffraction, scanning electron microscope, Fourier transform infrared spectroscopy, protein electrophoresis, thermogravimetry and differential scanning calorimetry, and amino acid analysis. During the dissolution process, the α-helix structure of rabbit hair was deconstructed, and the disulfide bond linkages were broken. The solubility of rabbit hair was significantly enhanced by increasing dissolution temperature, and reached 88% at 120 °C. The keratin produced by dissolving at 120 °C displayed flaky powders after freeze-drying, and had a molecular weight ranging from 3.8 to 5.8 kDa with a high proportion of serine, glutamic acid, cysteine, leucine, and arginine. Such features of molecular weight and amino acid distribution provide more choices for the diverse applications of keratin materials. MDPI 2018-09-06 /pmc/articles/PMC6404039/ /pubmed/30960918 http://dx.doi.org/10.3390/polym10090993 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Dongyue Yang, Xu-Hong Tang, Ren-Cheng Yao, Fan Extraction of Keratin from Rabbit Hair by a Deep Eutectic Solvent and Its Characterization |
title | Extraction of Keratin from Rabbit Hair by a Deep Eutectic Solvent and Its Characterization |
title_full | Extraction of Keratin from Rabbit Hair by a Deep Eutectic Solvent and Its Characterization |
title_fullStr | Extraction of Keratin from Rabbit Hair by a Deep Eutectic Solvent and Its Characterization |
title_full_unstemmed | Extraction of Keratin from Rabbit Hair by a Deep Eutectic Solvent and Its Characterization |
title_short | Extraction of Keratin from Rabbit Hair by a Deep Eutectic Solvent and Its Characterization |
title_sort | extraction of keratin from rabbit hair by a deep eutectic solvent and its characterization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404039/ https://www.ncbi.nlm.nih.gov/pubmed/30960918 http://dx.doi.org/10.3390/polym10090993 |
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