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Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests

BACKGROUND: In the presented study we investigated the development of the humoral immune response against LSDV during the process of re-vaccination of cattle over a time span of 5 months. In addition, the performance of different serological techniques for antibody detection against LSDV was compare...

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Autores principales: Milovanović, Milovan, Dietze, Klaas, Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Moritz, Tom, Hoffmann, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404298/
https://www.ncbi.nlm.nih.gov/pubmed/30841894
http://dx.doi.org/10.1186/s12917-019-1831-y
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author Milovanović, Milovan
Dietze, Klaas
Milićević, Vesna
Radojičić, Sonja
Valčić, Miroslav
Moritz, Tom
Hoffmann, Bernd
author_facet Milovanović, Milovan
Dietze, Klaas
Milićević, Vesna
Radojičić, Sonja
Valčić, Miroslav
Moritz, Tom
Hoffmann, Bernd
author_sort Milovanović, Milovan
collection PubMed
description BACKGROUND: In the presented study we investigated the development of the humoral immune response against LSDV during the process of re-vaccination of cattle over a time span of 5 months. In addition, the performance of different serological techniques for antibody detection against LSDV was compared. For sample collection, an area without previous LSD outbreak reports in Serbia was selected. Seventy-nine cattle from twenty farms vaccinated in 2016 and re-vaccinated in 2017 were included in the study. Two farms from the same area with good calving management were selected for investigation of passive LSDV antibody transfer from vaccinated mothers to new-borne calves. RESULTS: All investigated cattle were healthy on the day of vaccination and during the whole study. Swelling at the injection site or other side effects of vaccination did not occur after re-vaccination in the study. Detection of LSD-specific antibodies was performed with the standard serological methods VNT and IFAT as well as a commercially available Capripox double antigen multi-species-ELISA. Capripoxvirus-specific antibodies were detected 46 to 47 weeks after vaccination in 2016, with VNT in 35.06% and with IFAT and ELISA in 33.77%. A secondary response was observed in all three tests 1 month after re-vaccination with a significant increase in seropositive animals compared to the results before re-vaccination. With all applied serological methods, the number of animals testing positive was significantly higher at 1 and 5 months post re-vaccination than before re-vaccination. No significant statistical difference (p > 0.05) was observed between the results of all three tests used. The sensitivity and specificity of ELISA was estimated to be Se(ELISA) 91% and Sp(ELISA) 87% calculated by the results of VNT and Se(ELISA) 88% and Sp(ELISA) 76% calculated by the results of IFAT. Passive antibody transfer from vaccinated mothers to new-born calves was investigated at 14 days after birth. Discrepancies for the detection of LSDV specific antibodies between cows and newborn calves at the age of 14 days were observed in VNT and IFAT, but not in ELISA. CONCLUSION: Of all tests used the commercially available ELISA shows to be the most useful for high throughput analysis compared to VNT or IFAT.
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spelling pubmed-64042982019-03-18 Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests Milovanović, Milovan Dietze, Klaas Milićević, Vesna Radojičić, Sonja Valčić, Miroslav Moritz, Tom Hoffmann, Bernd BMC Vet Res Research Article BACKGROUND: In the presented study we investigated the development of the humoral immune response against LSDV during the process of re-vaccination of cattle over a time span of 5 months. In addition, the performance of different serological techniques for antibody detection against LSDV was compared. For sample collection, an area without previous LSD outbreak reports in Serbia was selected. Seventy-nine cattle from twenty farms vaccinated in 2016 and re-vaccinated in 2017 were included in the study. Two farms from the same area with good calving management were selected for investigation of passive LSDV antibody transfer from vaccinated mothers to new-borne calves. RESULTS: All investigated cattle were healthy on the day of vaccination and during the whole study. Swelling at the injection site or other side effects of vaccination did not occur after re-vaccination in the study. Detection of LSD-specific antibodies was performed with the standard serological methods VNT and IFAT as well as a commercially available Capripox double antigen multi-species-ELISA. Capripoxvirus-specific antibodies were detected 46 to 47 weeks after vaccination in 2016, with VNT in 35.06% and with IFAT and ELISA in 33.77%. A secondary response was observed in all three tests 1 month after re-vaccination with a significant increase in seropositive animals compared to the results before re-vaccination. With all applied serological methods, the number of animals testing positive was significantly higher at 1 and 5 months post re-vaccination than before re-vaccination. No significant statistical difference (p > 0.05) was observed between the results of all three tests used. The sensitivity and specificity of ELISA was estimated to be Se(ELISA) 91% and Sp(ELISA) 87% calculated by the results of VNT and Se(ELISA) 88% and Sp(ELISA) 76% calculated by the results of IFAT. Passive antibody transfer from vaccinated mothers to new-born calves was investigated at 14 days after birth. Discrepancies for the detection of LSDV specific antibodies between cows and newborn calves at the age of 14 days were observed in VNT and IFAT, but not in ELISA. CONCLUSION: Of all tests used the commercially available ELISA shows to be the most useful for high throughput analysis compared to VNT or IFAT. BioMed Central 2019-03-06 /pmc/articles/PMC6404298/ /pubmed/30841894 http://dx.doi.org/10.1186/s12917-019-1831-y Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Milovanović, Milovan
Dietze, Klaas
Milićević, Vesna
Radojičić, Sonja
Valčić, Miroslav
Moritz, Tom
Hoffmann, Bernd
Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests
title Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests
title_full Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests
title_fullStr Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests
title_full_unstemmed Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests
title_short Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests
title_sort humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404298/
https://www.ncbi.nlm.nih.gov/pubmed/30841894
http://dx.doi.org/10.1186/s12917-019-1831-y
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