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Mouse strains and sexual divergence in corneal innervation and nerve regeneration

A variety of mouse strains and sexes are used in studies of corneal wound healing and nerve regeneration. However, there is a gap of knowledge about corneal nerve density and its function in different mouse strains and sexes. In this study, we report a strain divergence of total and substance P (SP)...

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Autores principales: Pham, Thang Luong, Kakazu, Azucena, He, Jiucheng, Bazan, Haydee E. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Federation of American Societies for Experimental Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404582/
https://www.ncbi.nlm.nih.gov/pubmed/30561223
http://dx.doi.org/10.1096/fj.201801957R
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author Pham, Thang Luong
Kakazu, Azucena
He, Jiucheng
Bazan, Haydee E. P.
author_facet Pham, Thang Luong
Kakazu, Azucena
He, Jiucheng
Bazan, Haydee E. P.
author_sort Pham, Thang Luong
collection PubMed
description A variety of mouse strains and sexes are used in studies of corneal wound healing and nerve regeneration. However, there is a gap of knowledge about corneal nerve density and its function in different mouse strains and sexes. In this study, we report a strain divergence of total and substance P (SP) sensory corneal nerves in uninjured mice. The BALB/c mouse showed the highest nerve density, corneal sensitivity, and tear volume followed by CFW and then C57BL/6. No differences were found in total nerves and SP-positive nerves between sexes. After injury damaged the corneal nerves, an important role for mouse strains, biologic sex, and their association to corneal nerve regeneration was identified. All female mice have a faster nerve regeneration rate than males. The molecular mechanism of this sexual divergence involves higher secretion neurotrophic factors in tears, which in turn modulate gene expression in trigeminal ganglion neurons. An important upstream signaling regulator was β-estradiol, and topical treatment with β-estradiol confirmed its function in corneal nerve regeneration. In conclusion, our study shows that the strain and sex of laboratory mice significantly affect the different indicators of corneal innervation and nerve regeneration. Researchers investigating corneal diseases should carefully consider these factors.—Pham, T. L., Kakazu, A., He, J., Bazan, H. E. P. Mouse strains and sexual divergence in corneal innervation and nerve regeneration.
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spelling pubmed-64045822019-03-12 Mouse strains and sexual divergence in corneal innervation and nerve regeneration Pham, Thang Luong Kakazu, Azucena He, Jiucheng Bazan, Haydee E. P. FASEB J Research A variety of mouse strains and sexes are used in studies of corneal wound healing and nerve regeneration. However, there is a gap of knowledge about corneal nerve density and its function in different mouse strains and sexes. In this study, we report a strain divergence of total and substance P (SP) sensory corneal nerves in uninjured mice. The BALB/c mouse showed the highest nerve density, corneal sensitivity, and tear volume followed by CFW and then C57BL/6. No differences were found in total nerves and SP-positive nerves between sexes. After injury damaged the corneal nerves, an important role for mouse strains, biologic sex, and their association to corneal nerve regeneration was identified. All female mice have a faster nerve regeneration rate than males. The molecular mechanism of this sexual divergence involves higher secretion neurotrophic factors in tears, which in turn modulate gene expression in trigeminal ganglion neurons. An important upstream signaling regulator was β-estradiol, and topical treatment with β-estradiol confirmed its function in corneal nerve regeneration. In conclusion, our study shows that the strain and sex of laboratory mice significantly affect the different indicators of corneal innervation and nerve regeneration. Researchers investigating corneal diseases should carefully consider these factors.—Pham, T. L., Kakazu, A., He, J., Bazan, H. E. P. Mouse strains and sexual divergence in corneal innervation and nerve regeneration. Federation of American Societies for Experimental Biology 2019-03 2018-12-18 /pmc/articles/PMC6404582/ /pubmed/30561223 http://dx.doi.org/10.1096/fj.201801957R Text en © The Author(s) https://creativecommons.org/licenses/by-nc-nd/2.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 2.0 International (CC BY-NC-ND 2.0) (https://creativecommons.org/licenses/by-nc-nd/2.0/) which permits noncommercial use, distribution, and reproduction in any medium, but prohibits the publication/distribution of derivative works, provided the original work is properly cited.
spellingShingle Research
Pham, Thang Luong
Kakazu, Azucena
He, Jiucheng
Bazan, Haydee E. P.
Mouse strains and sexual divergence in corneal innervation and nerve regeneration
title Mouse strains and sexual divergence in corneal innervation and nerve regeneration
title_full Mouse strains and sexual divergence in corneal innervation and nerve regeneration
title_fullStr Mouse strains and sexual divergence in corneal innervation and nerve regeneration
title_full_unstemmed Mouse strains and sexual divergence in corneal innervation and nerve regeneration
title_short Mouse strains and sexual divergence in corneal innervation and nerve regeneration
title_sort mouse strains and sexual divergence in corneal innervation and nerve regeneration
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404582/
https://www.ncbi.nlm.nih.gov/pubmed/30561223
http://dx.doi.org/10.1096/fj.201801957R
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