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Construction of a high-density linkage map and QTL mapping for important agronomic traits in Stylosanthes guianensis (Aubl.) Sw.

Stylosanthes guianensis (Aubl.) Sw. is an economically important pasture and forage legume in tropical regions of the world. Genetic improvement of the crop can be enhanced through marker-assisted breeding. However, neither single nucleotide polymorphism (SNP) markers nor SNP-based genetic linkage m...

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Autores principales: Tang, Yan-Qiong, Xia, Zhi-Qiang, Ding, Ze-Ting, Ding, Ya-Cao, Liu, Zhu, Ma, Xiang, Liu, Jin-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405868/
https://www.ncbi.nlm.nih.gov/pubmed/30846860
http://dx.doi.org/10.1038/s41598-019-40489-7
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author Tang, Yan-Qiong
Xia, Zhi-Qiang
Ding, Ze-Ting
Ding, Ya-Cao
Liu, Zhu
Ma, Xiang
Liu, Jin-Ping
author_facet Tang, Yan-Qiong
Xia, Zhi-Qiang
Ding, Ze-Ting
Ding, Ya-Cao
Liu, Zhu
Ma, Xiang
Liu, Jin-Ping
author_sort Tang, Yan-Qiong
collection PubMed
description Stylosanthes guianensis (Aubl.) Sw. is an economically important pasture and forage legume in tropical regions of the world. Genetic improvement of the crop can be enhanced through marker-assisted breeding. However, neither single nucleotide polymorphism (SNP) markers nor SNP-based genetic linkage map has been previously reported. In this study, a high-quality genetic linkage map of 2572 SNP markers for S. guianensis is generated using amplified-fragment single nucleotide polymorphism and methylation (AFSM) approach. The genetic map has 10 linkage groups (LGs), which spanned 2226.6 cM, with an average genetic distance of 0.87 cM between adjacent markers. Genetic mapping of quantitative trait loci (QTLs) for important agronomic traits such as yield-related and nutritional or quality-related traits was performed using F(2) progeny of a cross between a male-sterile female parent TPRC1979 and male parent TPRCR273 with contrasting phenotypes for morphological and physiological traits. A total of 30 QTLs for 8 yield-related traits and 18 QTLs for 4 nutritional or quality-related traits are mapped on the linkage map. Both the high-quality genetic linkage map and the QTL mapping for important agronomic traits described here will provide valuable genetic resources for marker-assisted selection for S. guianensis.
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spelling pubmed-64058682019-03-11 Construction of a high-density linkage map and QTL mapping for important agronomic traits in Stylosanthes guianensis (Aubl.) Sw. Tang, Yan-Qiong Xia, Zhi-Qiang Ding, Ze-Ting Ding, Ya-Cao Liu, Zhu Ma, Xiang Liu, Jin-Ping Sci Rep Article Stylosanthes guianensis (Aubl.) Sw. is an economically important pasture and forage legume in tropical regions of the world. Genetic improvement of the crop can be enhanced through marker-assisted breeding. However, neither single nucleotide polymorphism (SNP) markers nor SNP-based genetic linkage map has been previously reported. In this study, a high-quality genetic linkage map of 2572 SNP markers for S. guianensis is generated using amplified-fragment single nucleotide polymorphism and methylation (AFSM) approach. The genetic map has 10 linkage groups (LGs), which spanned 2226.6 cM, with an average genetic distance of 0.87 cM between adjacent markers. Genetic mapping of quantitative trait loci (QTLs) for important agronomic traits such as yield-related and nutritional or quality-related traits was performed using F(2) progeny of a cross between a male-sterile female parent TPRC1979 and male parent TPRCR273 with contrasting phenotypes for morphological and physiological traits. A total of 30 QTLs for 8 yield-related traits and 18 QTLs for 4 nutritional or quality-related traits are mapped on the linkage map. Both the high-quality genetic linkage map and the QTL mapping for important agronomic traits described here will provide valuable genetic resources for marker-assisted selection for S. guianensis. Nature Publishing Group UK 2019-03-07 /pmc/articles/PMC6405868/ /pubmed/30846860 http://dx.doi.org/10.1038/s41598-019-40489-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tang, Yan-Qiong
Xia, Zhi-Qiang
Ding, Ze-Ting
Ding, Ya-Cao
Liu, Zhu
Ma, Xiang
Liu, Jin-Ping
Construction of a high-density linkage map and QTL mapping for important agronomic traits in Stylosanthes guianensis (Aubl.) Sw.
title Construction of a high-density linkage map and QTL mapping for important agronomic traits in Stylosanthes guianensis (Aubl.) Sw.
title_full Construction of a high-density linkage map and QTL mapping for important agronomic traits in Stylosanthes guianensis (Aubl.) Sw.
title_fullStr Construction of a high-density linkage map and QTL mapping for important agronomic traits in Stylosanthes guianensis (Aubl.) Sw.
title_full_unstemmed Construction of a high-density linkage map and QTL mapping for important agronomic traits in Stylosanthes guianensis (Aubl.) Sw.
title_short Construction of a high-density linkage map and QTL mapping for important agronomic traits in Stylosanthes guianensis (Aubl.) Sw.
title_sort construction of a high-density linkage map and qtl mapping for important agronomic traits in stylosanthes guianensis (aubl.) sw.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405868/
https://www.ncbi.nlm.nih.gov/pubmed/30846860
http://dx.doi.org/10.1038/s41598-019-40489-7
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