Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library

Bioassay of recombinant protein products is important tests to ensure protein effectiveness. Some recombinant protein products have no cells used in their bioassay but instead use animal models, while others have no suitable method. Here, we developed a method to obtain responsive cells used in bioa...

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Autores principales: Qin, Xi, Yao, Wenrong, Shi, Xinchang, Liu, Lan, Huang, Fang, Ding, Youxue, Zhou, Yong, Yu, Lei, Jia, Chuncui, Li, Shanhu, Rao, Chunming, Wang, Junzhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405917/
https://www.ncbi.nlm.nih.gov/pubmed/30846752
http://dx.doi.org/10.1038/s41598-019-40381-4
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author Qin, Xi
Yao, Wenrong
Shi, Xinchang
Liu, Lan
Huang, Fang
Ding, Youxue
Zhou, Yong
Yu, Lei
Jia, Chuncui
Li, Shanhu
Rao, Chunming
Wang, Junzhi
author_facet Qin, Xi
Yao, Wenrong
Shi, Xinchang
Liu, Lan
Huang, Fang
Ding, Youxue
Zhou, Yong
Yu, Lei
Jia, Chuncui
Li, Shanhu
Rao, Chunming
Wang, Junzhi
author_sort Qin, Xi
collection PubMed
description Bioassay of recombinant protein products is important tests to ensure protein effectiveness. Some recombinant protein products have no cells used in their bioassay but instead use animal models, while others have no suitable method. Here, we developed a method to obtain responsive cells used in bioassay of proteins. After screening of a CRISPR/Cas9 library, we obtained a responsive cell line that grew faster in the presence of rhEGF (recombinant human epidermal growth factor) than that of control cells. We used this cell line for bioassay of rhEGF. This cell line, compared with the control cells, had a 2 day shorter operation time and had lower interference. The responsive cell line is more suitable for use in bioassay of rhEGF.
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spelling pubmed-64059172019-03-12 Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library Qin, Xi Yao, Wenrong Shi, Xinchang Liu, Lan Huang, Fang Ding, Youxue Zhou, Yong Yu, Lei Jia, Chuncui Li, Shanhu Rao, Chunming Wang, Junzhi Sci Rep Article Bioassay of recombinant protein products is important tests to ensure protein effectiveness. Some recombinant protein products have no cells used in their bioassay but instead use animal models, while others have no suitable method. Here, we developed a method to obtain responsive cells used in bioassay of proteins. After screening of a CRISPR/Cas9 library, we obtained a responsive cell line that grew faster in the presence of rhEGF (recombinant human epidermal growth factor) than that of control cells. We used this cell line for bioassay of rhEGF. This cell line, compared with the control cells, had a 2 day shorter operation time and had lower interference. The responsive cell line is more suitable for use in bioassay of rhEGF. Nature Publishing Group UK 2019-03-07 /pmc/articles/PMC6405917/ /pubmed/30846752 http://dx.doi.org/10.1038/s41598-019-40381-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Qin, Xi
Yao, Wenrong
Shi, Xinchang
Liu, Lan
Huang, Fang
Ding, Youxue
Zhou, Yong
Yu, Lei
Jia, Chuncui
Li, Shanhu
Rao, Chunming
Wang, Junzhi
Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library
title Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library
title_full Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library
title_fullStr Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library
title_full_unstemmed Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library
title_short Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library
title_sort responsive cells for rhegf bioassay obtained through screening of a crispr/cas9 library
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405917/
https://www.ncbi.nlm.nih.gov/pubmed/30846752
http://dx.doi.org/10.1038/s41598-019-40381-4
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