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Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation
Background: Berberine (BBR) is a pharmaceutical chemical with a broad antibacterial spectrum, and its biological treatment has been of research and practical interest. In this study, a pure bacterial strain B16 was isolated from the activated sludge in a pharmaceutical wastewater treatment plant. Th...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406382/ https://www.ncbi.nlm.nih.gov/pubmed/30795638 http://dx.doi.org/10.3390/ijerph16040646 |
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author | Liu, Shiyue Zhang, Yi Zeng, Ping Wang, Heli Song, Yonghui Li, Juan |
author_facet | Liu, Shiyue Zhang, Yi Zeng, Ping Wang, Heli Song, Yonghui Li, Juan |
author_sort | Liu, Shiyue |
collection | PubMed |
description | Background: Berberine (BBR) is a pharmaceutical chemical with a broad antibacterial spectrum, and its biological treatment has been of research and practical interest. In this study, a pure bacterial strain B16 was isolated from the activated sludge in a pharmaceutical wastewater treatment plant. The aim of the study is to characterize the properties of the strain B16, especially its BBR degradation capability. Methods: The identification of strain B16 was conducted by visual observation, as well as biochemical and phylogenetic analysis. The degradation kinetics of strain B16 was tentatively described by Haldane model. Results: The strain B16 was 100% determined as a Sphingopyxis sp. The kinetic parameters of BBR degradation by strain B16 were as follows: Vmax 54.73 ± 5.54 mg (g MLSS · h)(−1), Km 66.68 ± 8.95 mg L(−1), and Ki 43.16 ± 5.92 mg L(−1), with an R(2) of 0.996. Stain B16 exhibited considerable capability of BBR degradation. BBR of initial concentration 40 mg L(−1) could be completely degraded in 48 h under optimal conditions. Conclusions: strain B16 was the first pure culture found with the ability to totally mineralize BBR, indicating the potential of B16 application in real industrial processes. |
format | Online Article Text |
id | pubmed-6406382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64063822019-03-21 Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation Liu, Shiyue Zhang, Yi Zeng, Ping Wang, Heli Song, Yonghui Li, Juan Int J Environ Res Public Health Article Background: Berberine (BBR) is a pharmaceutical chemical with a broad antibacterial spectrum, and its biological treatment has been of research and practical interest. In this study, a pure bacterial strain B16 was isolated from the activated sludge in a pharmaceutical wastewater treatment plant. The aim of the study is to characterize the properties of the strain B16, especially its BBR degradation capability. Methods: The identification of strain B16 was conducted by visual observation, as well as biochemical and phylogenetic analysis. The degradation kinetics of strain B16 was tentatively described by Haldane model. Results: The strain B16 was 100% determined as a Sphingopyxis sp. The kinetic parameters of BBR degradation by strain B16 were as follows: Vmax 54.73 ± 5.54 mg (g MLSS · h)(−1), Km 66.68 ± 8.95 mg L(−1), and Ki 43.16 ± 5.92 mg L(−1), with an R(2) of 0.996. Stain B16 exhibited considerable capability of BBR degradation. BBR of initial concentration 40 mg L(−1) could be completely degraded in 48 h under optimal conditions. Conclusions: strain B16 was the first pure culture found with the ability to totally mineralize BBR, indicating the potential of B16 application in real industrial processes. MDPI 2019-02-21 2019-02 /pmc/articles/PMC6406382/ /pubmed/30795638 http://dx.doi.org/10.3390/ijerph16040646 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Shiyue Zhang, Yi Zeng, Ping Wang, Heli Song, Yonghui Li, Juan Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation |
title | Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation |
title_full | Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation |
title_fullStr | Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation |
title_full_unstemmed | Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation |
title_short | Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation |
title_sort | isolation and characterization of a bacterial strain capable of efficient berberine degradation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406382/ https://www.ncbi.nlm.nih.gov/pubmed/30795638 http://dx.doi.org/10.3390/ijerph16040646 |
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