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Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation

Background: Berberine (BBR) is a pharmaceutical chemical with a broad antibacterial spectrum, and its biological treatment has been of research and practical interest. In this study, a pure bacterial strain B16 was isolated from the activated sludge in a pharmaceutical wastewater treatment plant. Th...

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Autores principales: Liu, Shiyue, Zhang, Yi, Zeng, Ping, Wang, Heli, Song, Yonghui, Li, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406382/
https://www.ncbi.nlm.nih.gov/pubmed/30795638
http://dx.doi.org/10.3390/ijerph16040646
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author Liu, Shiyue
Zhang, Yi
Zeng, Ping
Wang, Heli
Song, Yonghui
Li, Juan
author_facet Liu, Shiyue
Zhang, Yi
Zeng, Ping
Wang, Heli
Song, Yonghui
Li, Juan
author_sort Liu, Shiyue
collection PubMed
description Background: Berberine (BBR) is a pharmaceutical chemical with a broad antibacterial spectrum, and its biological treatment has been of research and practical interest. In this study, a pure bacterial strain B16 was isolated from the activated sludge in a pharmaceutical wastewater treatment plant. The aim of the study is to characterize the properties of the strain B16, especially its BBR degradation capability. Methods: The identification of strain B16 was conducted by visual observation, as well as biochemical and phylogenetic analysis. The degradation kinetics of strain B16 was tentatively described by Haldane model. Results: The strain B16 was 100% determined as a Sphingopyxis sp. The kinetic parameters of BBR degradation by strain B16 were as follows: Vmax 54.73 ± 5.54 mg (g MLSS · h)(−1), Km 66.68 ± 8.95 mg L(−1), and Ki 43.16 ± 5.92 mg L(−1), with an R(2) of 0.996. Stain B16 exhibited considerable capability of BBR degradation. BBR of initial concentration 40 mg L(−1) could be completely degraded in 48 h under optimal conditions. Conclusions: strain B16 was the first pure culture found with the ability to totally mineralize BBR, indicating the potential of B16 application in real industrial processes.
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spelling pubmed-64063822019-03-21 Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation Liu, Shiyue Zhang, Yi Zeng, Ping Wang, Heli Song, Yonghui Li, Juan Int J Environ Res Public Health Article Background: Berberine (BBR) is a pharmaceutical chemical with a broad antibacterial spectrum, and its biological treatment has been of research and practical interest. In this study, a pure bacterial strain B16 was isolated from the activated sludge in a pharmaceutical wastewater treatment plant. The aim of the study is to characterize the properties of the strain B16, especially its BBR degradation capability. Methods: The identification of strain B16 was conducted by visual observation, as well as biochemical and phylogenetic analysis. The degradation kinetics of strain B16 was tentatively described by Haldane model. Results: The strain B16 was 100% determined as a Sphingopyxis sp. The kinetic parameters of BBR degradation by strain B16 were as follows: Vmax 54.73 ± 5.54 mg (g MLSS · h)(−1), Km 66.68 ± 8.95 mg L(−1), and Ki 43.16 ± 5.92 mg L(−1), with an R(2) of 0.996. Stain B16 exhibited considerable capability of BBR degradation. BBR of initial concentration 40 mg L(−1) could be completely degraded in 48 h under optimal conditions. Conclusions: strain B16 was the first pure culture found with the ability to totally mineralize BBR, indicating the potential of B16 application in real industrial processes. MDPI 2019-02-21 2019-02 /pmc/articles/PMC6406382/ /pubmed/30795638 http://dx.doi.org/10.3390/ijerph16040646 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Shiyue
Zhang, Yi
Zeng, Ping
Wang, Heli
Song, Yonghui
Li, Juan
Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation
title Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation
title_full Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation
title_fullStr Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation
title_full_unstemmed Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation
title_short Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation
title_sort isolation and characterization of a bacterial strain capable of efficient berberine degradation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406382/
https://www.ncbi.nlm.nih.gov/pubmed/30795638
http://dx.doi.org/10.3390/ijerph16040646
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