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Manganese Suppresses the Haploinsufficiency of Heterozygous trpy1Δ/TRPY1 Saccharomyces cerevisiae Cells and Stimulates the TRPY1-Dependent Release of Vacuolar Ca(2+) under H(2)O(2) Stress

Transient potential receptor (TRP) channels are conserved cation channels found in most eukaryotes, known to sense a variety of chemical, thermal or mechanical stimuli. The Saccharomyces cerevisiae TRPY1 is a TRP channel with vacuolar localization involved in the cellular response to hyperosmotic sh...

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Autores principales: Ruta, Lavinia L., Nicolau, Ioana, Popa, Claudia V., Farcasanu, Ileana C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406398/
https://www.ncbi.nlm.nih.gov/pubmed/30678234
http://dx.doi.org/10.3390/cells8020079
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author Ruta, Lavinia L.
Nicolau, Ioana
Popa, Claudia V.
Farcasanu, Ileana C.
author_facet Ruta, Lavinia L.
Nicolau, Ioana
Popa, Claudia V.
Farcasanu, Ileana C.
author_sort Ruta, Lavinia L.
collection PubMed
description Transient potential receptor (TRP) channels are conserved cation channels found in most eukaryotes, known to sense a variety of chemical, thermal or mechanical stimuli. The Saccharomyces cerevisiae TRPY1 is a TRP channel with vacuolar localization involved in the cellular response to hyperosmotic shock and oxidative stress. In this study, we found that S. cerevisiae diploid cells with heterozygous deletion in TRPY1 gene are haploinsufficient when grown in synthetic media deficient in essential metal ions and that this growth defect is alleviated by non-toxic Mn(2+) surplus. Using cells expressing the Ca(2+)-sensitive photoprotein aequorin we found that Mn(2+) augmented the Ca(2+) flux into the cytosol under oxidative stress, but not under hyperosmotic shock, a trait that was absent in the diploid cells with homozygous deletion of TRPY1 gene. TRPY1 activation under oxidative stress was diminished in cells devoid of Smf1 (the Mn(2+)-high-affinity plasma membrane transporter) but it was clearly augmented in cells lacking Pmr1 (the endoplasmic reticulum (ER)/Golgi located ATPase responsible for Mn(2+) detoxification via excretory pathway). Taken together, these observations lead to the conclusion that increased levels of intracytosolic Mn(2+) activate TRPY1 in the response to oxidative stress.
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spelling pubmed-64063982019-03-19 Manganese Suppresses the Haploinsufficiency of Heterozygous trpy1Δ/TRPY1 Saccharomyces cerevisiae Cells and Stimulates the TRPY1-Dependent Release of Vacuolar Ca(2+) under H(2)O(2) Stress Ruta, Lavinia L. Nicolau, Ioana Popa, Claudia V. Farcasanu, Ileana C. Cells Article Transient potential receptor (TRP) channels are conserved cation channels found in most eukaryotes, known to sense a variety of chemical, thermal or mechanical stimuli. The Saccharomyces cerevisiae TRPY1 is a TRP channel with vacuolar localization involved in the cellular response to hyperosmotic shock and oxidative stress. In this study, we found that S. cerevisiae diploid cells with heterozygous deletion in TRPY1 gene are haploinsufficient when grown in synthetic media deficient in essential metal ions and that this growth defect is alleviated by non-toxic Mn(2+) surplus. Using cells expressing the Ca(2+)-sensitive photoprotein aequorin we found that Mn(2+) augmented the Ca(2+) flux into the cytosol under oxidative stress, but not under hyperosmotic shock, a trait that was absent in the diploid cells with homozygous deletion of TRPY1 gene. TRPY1 activation under oxidative stress was diminished in cells devoid of Smf1 (the Mn(2+)-high-affinity plasma membrane transporter) but it was clearly augmented in cells lacking Pmr1 (the endoplasmic reticulum (ER)/Golgi located ATPase responsible for Mn(2+) detoxification via excretory pathway). Taken together, these observations lead to the conclusion that increased levels of intracytosolic Mn(2+) activate TRPY1 in the response to oxidative stress. MDPI 2019-01-22 /pmc/articles/PMC6406398/ /pubmed/30678234 http://dx.doi.org/10.3390/cells8020079 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ruta, Lavinia L.
Nicolau, Ioana
Popa, Claudia V.
Farcasanu, Ileana C.
Manganese Suppresses the Haploinsufficiency of Heterozygous trpy1Δ/TRPY1 Saccharomyces cerevisiae Cells and Stimulates the TRPY1-Dependent Release of Vacuolar Ca(2+) under H(2)O(2) Stress
title Manganese Suppresses the Haploinsufficiency of Heterozygous trpy1Δ/TRPY1 Saccharomyces cerevisiae Cells and Stimulates the TRPY1-Dependent Release of Vacuolar Ca(2+) under H(2)O(2) Stress
title_full Manganese Suppresses the Haploinsufficiency of Heterozygous trpy1Δ/TRPY1 Saccharomyces cerevisiae Cells and Stimulates the TRPY1-Dependent Release of Vacuolar Ca(2+) under H(2)O(2) Stress
title_fullStr Manganese Suppresses the Haploinsufficiency of Heterozygous trpy1Δ/TRPY1 Saccharomyces cerevisiae Cells and Stimulates the TRPY1-Dependent Release of Vacuolar Ca(2+) under H(2)O(2) Stress
title_full_unstemmed Manganese Suppresses the Haploinsufficiency of Heterozygous trpy1Δ/TRPY1 Saccharomyces cerevisiae Cells and Stimulates the TRPY1-Dependent Release of Vacuolar Ca(2+) under H(2)O(2) Stress
title_short Manganese Suppresses the Haploinsufficiency of Heterozygous trpy1Δ/TRPY1 Saccharomyces cerevisiae Cells and Stimulates the TRPY1-Dependent Release of Vacuolar Ca(2+) under H(2)O(2) Stress
title_sort manganese suppresses the haploinsufficiency of heterozygous trpy1δ/trpy1 saccharomyces cerevisiae cells and stimulates the trpy1-dependent release of vacuolar ca(2+) under h(2)o(2) stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406398/
https://www.ncbi.nlm.nih.gov/pubmed/30678234
http://dx.doi.org/10.3390/cells8020079
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