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Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica

Recombinant protein production represents a multibillion-dollar market. Therefore, it constitutes an important research field both in academia and industry. The use of yeast as a cell factory presents several advantages such as ease of genetic manipulation, growth at high cell density, and the possi...

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Autores principales: Vandermies, Marie, Fickers, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406515/
https://www.ncbi.nlm.nih.gov/pubmed/30704141
http://dx.doi.org/10.3390/microorganisms7020040
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author Vandermies, Marie
Fickers, Patrick
author_facet Vandermies, Marie
Fickers, Patrick
author_sort Vandermies, Marie
collection PubMed
description Recombinant protein production represents a multibillion-dollar market. Therefore, it constitutes an important research field both in academia and industry. The use of yeast as a cell factory presents several advantages such as ease of genetic manipulation, growth at high cell density, and the possibility of post-translational modifications. Yarrowia lipolytica is considered as one of the most attractive hosts due to its ability to metabolize raw substrate, to express genes at a high level, and to secrete protein in large amounts. In recent years, several reviews have been dedicated to genetic tools developed for this purpose. Though the construction of efficient cell factories for recombinant protein synthesis is important, the development of an efficient process for recombinant protein production in a bioreactor constitutes an equally vital aspect. Indeed, a sports car cannot drive fast on a gravel road. The aim of this review is to provide a comprehensive snapshot of process tools to consider for recombinant protein production in bioreactor using Y. lipolytica as a cell factory, in order to facilitate the decision-making for future strain and process engineering.
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spelling pubmed-64065152019-03-26 Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica Vandermies, Marie Fickers, Patrick Microorganisms Review Recombinant protein production represents a multibillion-dollar market. Therefore, it constitutes an important research field both in academia and industry. The use of yeast as a cell factory presents several advantages such as ease of genetic manipulation, growth at high cell density, and the possibility of post-translational modifications. Yarrowia lipolytica is considered as one of the most attractive hosts due to its ability to metabolize raw substrate, to express genes at a high level, and to secrete protein in large amounts. In recent years, several reviews have been dedicated to genetic tools developed for this purpose. Though the construction of efficient cell factories for recombinant protein synthesis is important, the development of an efficient process for recombinant protein production in a bioreactor constitutes an equally vital aspect. Indeed, a sports car cannot drive fast on a gravel road. The aim of this review is to provide a comprehensive snapshot of process tools to consider for recombinant protein production in bioreactor using Y. lipolytica as a cell factory, in order to facilitate the decision-making for future strain and process engineering. MDPI 2019-01-30 /pmc/articles/PMC6406515/ /pubmed/30704141 http://dx.doi.org/10.3390/microorganisms7020040 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Vandermies, Marie
Fickers, Patrick
Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica
title Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica
title_full Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica
title_fullStr Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica
title_full_unstemmed Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica
title_short Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica
title_sort bioreactor-scale strategies for the production of recombinant protein in the yeast yarrowia lipolytica
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406515/
https://www.ncbi.nlm.nih.gov/pubmed/30704141
http://dx.doi.org/10.3390/microorganisms7020040
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