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Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci
Mastitis poses a considerable threat to productivity and to animal welfare on modern dairy farms. However, the common way of antibiotic treatment does not always lead to a cure. Unsuccessful cures can, among other reasons, occur due to biofilm formation of the causative agent. This has attracted int...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406540/ https://www.ncbi.nlm.nih.gov/pubmed/30791514 http://dx.doi.org/10.3390/foods8020079 |
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author | Wallis, Jonathan K. Krömker, Volker Paduch, Jan-Hendrik |
author_facet | Wallis, Jonathan K. Krömker, Volker Paduch, Jan-Hendrik |
author_sort | Wallis, Jonathan K. |
collection | PubMed |
description | Mastitis poses a considerable threat to productivity and to animal welfare on modern dairy farms. However, the common way of antibiotic treatment does not always lead to a cure. Unsuccessful cures can, among other reasons, occur due to biofilm formation of the causative agent. This has attracted interest from researchers to introduce promising alternative therapeutic approaches, such as the use of beneficial lactic acid bacteria (LAB). In fact, using LAB for treating mastitis probably requires the formation of a beneficial biofilm by the probiotic bacteria. The present study investigated the ability of five LAB strains, selected on the basis of results from previous studies, to remove and to replace pathogenic biofilms in vitro. For this purpose, Staphylococcus (S.) aureus ATCC 12,600 and two strains—S. xylosus (35/07) and S. epidermidis (575/08)—belonging to the group of coagulase negative staphylococci (CNS) were allowed to form biofilms in a 96-well plate. Subsequently, the LAB were added to the well. The biofilm challenge was evaluated by scraping off and suspending the biofilm cells, followed by a plate count of serial dilutions using selective media. All the LAB strains successfully removed the staphylococcal biofilms. However, only Lactobacillus (L.) rhamnosus ATCC 7469 and L. plantarum 2/37 formed biofilms of their own to replace the pathogenic ones. |
format | Online Article Text |
id | pubmed-6406540 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64065402019-03-19 Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci Wallis, Jonathan K. Krömker, Volker Paduch, Jan-Hendrik Foods Article Mastitis poses a considerable threat to productivity and to animal welfare on modern dairy farms. However, the common way of antibiotic treatment does not always lead to a cure. Unsuccessful cures can, among other reasons, occur due to biofilm formation of the causative agent. This has attracted interest from researchers to introduce promising alternative therapeutic approaches, such as the use of beneficial lactic acid bacteria (LAB). In fact, using LAB for treating mastitis probably requires the formation of a beneficial biofilm by the probiotic bacteria. The present study investigated the ability of five LAB strains, selected on the basis of results from previous studies, to remove and to replace pathogenic biofilms in vitro. For this purpose, Staphylococcus (S.) aureus ATCC 12,600 and two strains—S. xylosus (35/07) and S. epidermidis (575/08)—belonging to the group of coagulase negative staphylococci (CNS) were allowed to form biofilms in a 96-well plate. Subsequently, the LAB were added to the well. The biofilm challenge was evaluated by scraping off and suspending the biofilm cells, followed by a plate count of serial dilutions using selective media. All the LAB strains successfully removed the staphylococcal biofilms. However, only Lactobacillus (L.) rhamnosus ATCC 7469 and L. plantarum 2/37 formed biofilms of their own to replace the pathogenic ones. MDPI 2019-02-20 /pmc/articles/PMC6406540/ /pubmed/30791514 http://dx.doi.org/10.3390/foods8020079 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wallis, Jonathan K. Krömker, Volker Paduch, Jan-Hendrik Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci |
title | Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci |
title_full | Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci |
title_fullStr | Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci |
title_full_unstemmed | Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci |
title_short | Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci |
title_sort | biofilm challenge: lactic acid bacteria isolated from bovine udders versus staphylococci |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406540/ https://www.ncbi.nlm.nih.gov/pubmed/30791514 http://dx.doi.org/10.3390/foods8020079 |
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