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Anti-Tumor Activity of Cembranoid-Type Diterpenes Isolated from Nicotiana tabacum L.
Recently, the incidence of hepatocellular carcinoma has increased worldwide. Cembranoid-type diterpenes (CBDs) from tobacco exhibit good antimicrobial, antitumor, and neuroprotective activities. Therefore, in this study, we isolated CBDs from Nicotiana tabacum L. and evaluated their antitumor activi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406568/ https://www.ncbi.nlm.nih.gov/pubmed/30696084 http://dx.doi.org/10.3390/biom9020045 |
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author | Yuan, Xiao-Long Mao, Xin-Xin Du, Yong-Mei Yan, Pei-Zhen Hou, Xiao-Dong Zhang, Zhong-Feng |
author_facet | Yuan, Xiao-Long Mao, Xin-Xin Du, Yong-Mei Yan, Pei-Zhen Hou, Xiao-Dong Zhang, Zhong-Feng |
author_sort | Yuan, Xiao-Long |
collection | PubMed |
description | Recently, the incidence of hepatocellular carcinoma has increased worldwide. Cembranoid-type diterpenes (CBDs) from tobacco exhibit good antimicrobial, antitumor, and neuroprotective activities. Therefore, in this study, we isolated CBDs from Nicotiana tabacum L. and evaluated their antitumor activity against hepatoma cell lines. Particularly, the anti-tumor activity of α-2,7,11-cyprotermine-4,6-diol (α-CBD) was investigated against HepG2, SMMC-7721, and HL-7702 cells. The MTT assay revealed that α-CBD reduced the formation of cell clones and inhibited the proliferation of hepatocellular carcinoma cells. Morphological observations showed that α-CBD altered cell morphology and membrane permeability before inducing apoptosis. To further explore the antitumor mechanism of α-CBD, flow cytometry and transcriptome analysis were performed using HepG2 cells. The results showed that the number of HepG2 cells increased from 10.4% to 29.8%, indicating that α-CBD inhibits the proliferation of hepatocellular carcinoma cells in the S phase. The gene expression analysis of HepG2 cells treated with α-CBD showed 3068 genes with altered expression, among which 1289 were upregulated and 1779 were downregulated. Apoptosis induced by these differentially expressed genes might be mediated by the p53-PUMA, PI3K-Akt, and IL-1-NF-κB-IAP pathways. Comprehensively, our study shows that α-CBD isolated from N. tabacum L. can be potentially used as a natural antitumor agent. |
format | Online Article Text |
id | pubmed-6406568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64065682019-03-13 Anti-Tumor Activity of Cembranoid-Type Diterpenes Isolated from Nicotiana tabacum L. Yuan, Xiao-Long Mao, Xin-Xin Du, Yong-Mei Yan, Pei-Zhen Hou, Xiao-Dong Zhang, Zhong-Feng Biomolecules Article Recently, the incidence of hepatocellular carcinoma has increased worldwide. Cembranoid-type diterpenes (CBDs) from tobacco exhibit good antimicrobial, antitumor, and neuroprotective activities. Therefore, in this study, we isolated CBDs from Nicotiana tabacum L. and evaluated their antitumor activity against hepatoma cell lines. Particularly, the anti-tumor activity of α-2,7,11-cyprotermine-4,6-diol (α-CBD) was investigated against HepG2, SMMC-7721, and HL-7702 cells. The MTT assay revealed that α-CBD reduced the formation of cell clones and inhibited the proliferation of hepatocellular carcinoma cells. Morphological observations showed that α-CBD altered cell morphology and membrane permeability before inducing apoptosis. To further explore the antitumor mechanism of α-CBD, flow cytometry and transcriptome analysis were performed using HepG2 cells. The results showed that the number of HepG2 cells increased from 10.4% to 29.8%, indicating that α-CBD inhibits the proliferation of hepatocellular carcinoma cells in the S phase. The gene expression analysis of HepG2 cells treated with α-CBD showed 3068 genes with altered expression, among which 1289 were upregulated and 1779 were downregulated. Apoptosis induced by these differentially expressed genes might be mediated by the p53-PUMA, PI3K-Akt, and IL-1-NF-κB-IAP pathways. Comprehensively, our study shows that α-CBD isolated from N. tabacum L. can be potentially used as a natural antitumor agent. MDPI 2019-01-28 /pmc/articles/PMC6406568/ /pubmed/30696084 http://dx.doi.org/10.3390/biom9020045 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yuan, Xiao-Long Mao, Xin-Xin Du, Yong-Mei Yan, Pei-Zhen Hou, Xiao-Dong Zhang, Zhong-Feng Anti-Tumor Activity of Cembranoid-Type Diterpenes Isolated from Nicotiana tabacum L. |
title | Anti-Tumor Activity of Cembranoid-Type Diterpenes Isolated from Nicotiana tabacum L. |
title_full | Anti-Tumor Activity of Cembranoid-Type Diterpenes Isolated from Nicotiana tabacum L. |
title_fullStr | Anti-Tumor Activity of Cembranoid-Type Diterpenes Isolated from Nicotiana tabacum L. |
title_full_unstemmed | Anti-Tumor Activity of Cembranoid-Type Diterpenes Isolated from Nicotiana tabacum L. |
title_short | Anti-Tumor Activity of Cembranoid-Type Diterpenes Isolated from Nicotiana tabacum L. |
title_sort | anti-tumor activity of cembranoid-type diterpenes isolated from nicotiana tabacum l. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406568/ https://www.ncbi.nlm.nih.gov/pubmed/30696084 http://dx.doi.org/10.3390/biom9020045 |
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