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Circulating MiRNA-195-5p and -451a in Transient and Acute Ischemic Stroke Patients in an Emergency Department

We have evaluated circulating miRNAs (-195-5p and -451a) in subjects with acute ischemic stroke (AIS) and in patients with transient ischemic attack (TIA). In this study, 18 subjects with AIS and 18 patients with TIA were enrolled and examined at admission (T0) and at 24 h and 48 h after admission,...

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Autores principales: Giordano, Mauro, Ciarambino, Tiziana, D’Amico, Michele, Trotta, Maria Consiglia, Di Sette, Alessandra Marinella, Marfella, Raffaele, Malatino, Lorenzo, Paolisso, Giuseppe, Adinolfi, Luigi Elio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406765/
https://www.ncbi.nlm.nih.gov/pubmed/30678250
http://dx.doi.org/10.3390/jcm8020130
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author Giordano, Mauro
Ciarambino, Tiziana
D’Amico, Michele
Trotta, Maria Consiglia
Di Sette, Alessandra Marinella
Marfella, Raffaele
Malatino, Lorenzo
Paolisso, Giuseppe
Adinolfi, Luigi Elio
author_facet Giordano, Mauro
Ciarambino, Tiziana
D’Amico, Michele
Trotta, Maria Consiglia
Di Sette, Alessandra Marinella
Marfella, Raffaele
Malatino, Lorenzo
Paolisso, Giuseppe
Adinolfi, Luigi Elio
author_sort Giordano, Mauro
collection PubMed
description We have evaluated circulating miRNAs (-195-5p and -451a) in subjects with acute ischemic stroke (AIS) and in patients with transient ischemic attack (TIA). In this study, 18 subjects with AIS and 18 patients with TIA were enrolled and examined at admission (T0) and at 24 h and 48 h after admission, and compared to 20 controls (C). At T0, circulating miRNA-195-5p and -451a were significantly upregulated in both AIS and TIA patients, compared to C. We also observed a progressive reduction of circulating miRNA levels at 24 h and 48 h in both AIS and TIA patients. Hypoxia inducible factor 1alpha (HIF-1α) serum level was significantly increased at T0, in both AIS and TIA patients, in comparison to C (both p < 0.01 vs. C) and it decreased in both AIS and TIA patients at 24 h and at 48 h, in comparison to T0 (both p < 0.01 vs. T0). Vascular endothelial growth factor (VEGF) serum level was significantly decreased at T0, in both AIS and TIA patients, if compared to C (both p < 0.01 vs. C) and increased, in both AIS and TIA patients, at 24 h and 48 h, if compared to T0 (both p < 0.01 vs. T0). The elevated expression of miRNA-195-5p and miRNA-451a significantly decreased over time at 24 h and 48 h, and it is associated with decreased HIF-α levels and increased VEGF serum levels. These data may suggest a role for this miRNAs as biomarker in the pathogenesis and prognosis of AIS patients and for the first time also in TIA patients.
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spelling pubmed-64067652019-03-22 Circulating MiRNA-195-5p and -451a in Transient and Acute Ischemic Stroke Patients in an Emergency Department Giordano, Mauro Ciarambino, Tiziana D’Amico, Michele Trotta, Maria Consiglia Di Sette, Alessandra Marinella Marfella, Raffaele Malatino, Lorenzo Paolisso, Giuseppe Adinolfi, Luigi Elio J Clin Med Article We have evaluated circulating miRNAs (-195-5p and -451a) in subjects with acute ischemic stroke (AIS) and in patients with transient ischemic attack (TIA). In this study, 18 subjects with AIS and 18 patients with TIA were enrolled and examined at admission (T0) and at 24 h and 48 h after admission, and compared to 20 controls (C). At T0, circulating miRNA-195-5p and -451a were significantly upregulated in both AIS and TIA patients, compared to C. We also observed a progressive reduction of circulating miRNA levels at 24 h and 48 h in both AIS and TIA patients. Hypoxia inducible factor 1alpha (HIF-1α) serum level was significantly increased at T0, in both AIS and TIA patients, in comparison to C (both p < 0.01 vs. C) and it decreased in both AIS and TIA patients at 24 h and at 48 h, in comparison to T0 (both p < 0.01 vs. T0). Vascular endothelial growth factor (VEGF) serum level was significantly decreased at T0, in both AIS and TIA patients, if compared to C (both p < 0.01 vs. C) and increased, in both AIS and TIA patients, at 24 h and 48 h, if compared to T0 (both p < 0.01 vs. T0). The elevated expression of miRNA-195-5p and miRNA-451a significantly decreased over time at 24 h and 48 h, and it is associated with decreased HIF-α levels and increased VEGF serum levels. These data may suggest a role for this miRNAs as biomarker in the pathogenesis and prognosis of AIS patients and for the first time also in TIA patients. MDPI 2019-01-22 /pmc/articles/PMC6406765/ /pubmed/30678250 http://dx.doi.org/10.3390/jcm8020130 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Giordano, Mauro
Ciarambino, Tiziana
D’Amico, Michele
Trotta, Maria Consiglia
Di Sette, Alessandra Marinella
Marfella, Raffaele
Malatino, Lorenzo
Paolisso, Giuseppe
Adinolfi, Luigi Elio
Circulating MiRNA-195-5p and -451a in Transient and Acute Ischemic Stroke Patients in an Emergency Department
title Circulating MiRNA-195-5p and -451a in Transient and Acute Ischemic Stroke Patients in an Emergency Department
title_full Circulating MiRNA-195-5p and -451a in Transient and Acute Ischemic Stroke Patients in an Emergency Department
title_fullStr Circulating MiRNA-195-5p and -451a in Transient and Acute Ischemic Stroke Patients in an Emergency Department
title_full_unstemmed Circulating MiRNA-195-5p and -451a in Transient and Acute Ischemic Stroke Patients in an Emergency Department
title_short Circulating MiRNA-195-5p and -451a in Transient and Acute Ischemic Stroke Patients in an Emergency Department
title_sort circulating mirna-195-5p and -451a in transient and acute ischemic stroke patients in an emergency department
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406765/
https://www.ncbi.nlm.nih.gov/pubmed/30678250
http://dx.doi.org/10.3390/jcm8020130
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