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Moscatilin Inhibits Growth of Human Esophageal Cancer Xenograft and Sensitizes Cancer Cells to Radiotherapy

Esophageal cancer prognosis remains poor in current clinical practice. We previously reported that moscatilin can induce apoptosis and mitotic catastrophe in esophageal cancer cells, accompanied by upregulation of polo-like kinase 1 (Plk1) expression. We aimed to validate in vitro activity and Plk1...

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Autores principales: Chen, Wun-Ke, Chen, Chien-An, Chi, Chih-Wen, Li, Li-Hui, Lin, Chin-Ping, Shieh, Hui-Ru, Hsu, Ming-Ling, Ko, Chun-Chuan, Hwang, Jeng-Jong, Chen, Yu-Jen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406854/
https://www.ncbi.nlm.nih.gov/pubmed/30764514
http://dx.doi.org/10.3390/jcm8020187
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author Chen, Wun-Ke
Chen, Chien-An
Chi, Chih-Wen
Li, Li-Hui
Lin, Chin-Ping
Shieh, Hui-Ru
Hsu, Ming-Ling
Ko, Chun-Chuan
Hwang, Jeng-Jong
Chen, Yu-Jen
author_facet Chen, Wun-Ke
Chen, Chien-An
Chi, Chih-Wen
Li, Li-Hui
Lin, Chin-Ping
Shieh, Hui-Ru
Hsu, Ming-Ling
Ko, Chun-Chuan
Hwang, Jeng-Jong
Chen, Yu-Jen
author_sort Chen, Wun-Ke
collection PubMed
description Esophageal cancer prognosis remains poor in current clinical practice. We previously reported that moscatilin can induce apoptosis and mitotic catastrophe in esophageal cancer cells, accompanied by upregulation of polo-like kinase 1 (Plk1) expression. We aimed to validate in vitro activity and Plk1 expression in vivo following moscatilin treatment and to examine the treatment’s radiosensitizing effect. Human esophageal cancer cells were implanted in nude mice. Moscatilin was intraperitoneally (i.p.) injected into the mice. Tumor size, body weight, white blood cell counts, and liver and renal function were measured. Aberrant mitosis and Plk1 expression were assessed. Colony formation was used to measure survival fraction after radiation. Moscatilin significantly suppressed tumor growth in mice bearing human esophageal xenografts without affecting body weight, white blood cell counts, or liver and renal function. Moscatilin also induced aberrant mitosis and apoptosis. Plk1 expression was markedly upregulated in vivo. Moreover, moscatilin pretreatment enhanced CE81T/VGH and BE3 cell radioresponse in vitro. Moscatilin may inhibit growth of human esophageal tumors and sensitize esophageal cancer cells to radiation therapy.
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spelling pubmed-64068542019-03-22 Moscatilin Inhibits Growth of Human Esophageal Cancer Xenograft and Sensitizes Cancer Cells to Radiotherapy Chen, Wun-Ke Chen, Chien-An Chi, Chih-Wen Li, Li-Hui Lin, Chin-Ping Shieh, Hui-Ru Hsu, Ming-Ling Ko, Chun-Chuan Hwang, Jeng-Jong Chen, Yu-Jen J Clin Med Article Esophageal cancer prognosis remains poor in current clinical practice. We previously reported that moscatilin can induce apoptosis and mitotic catastrophe in esophageal cancer cells, accompanied by upregulation of polo-like kinase 1 (Plk1) expression. We aimed to validate in vitro activity and Plk1 expression in vivo following moscatilin treatment and to examine the treatment’s radiosensitizing effect. Human esophageal cancer cells were implanted in nude mice. Moscatilin was intraperitoneally (i.p.) injected into the mice. Tumor size, body weight, white blood cell counts, and liver and renal function were measured. Aberrant mitosis and Plk1 expression were assessed. Colony formation was used to measure survival fraction after radiation. Moscatilin significantly suppressed tumor growth in mice bearing human esophageal xenografts without affecting body weight, white blood cell counts, or liver and renal function. Moscatilin also induced aberrant mitosis and apoptosis. Plk1 expression was markedly upregulated in vivo. Moreover, moscatilin pretreatment enhanced CE81T/VGH and BE3 cell radioresponse in vitro. Moscatilin may inhibit growth of human esophageal tumors and sensitize esophageal cancer cells to radiation therapy. MDPI 2019-02-05 /pmc/articles/PMC6406854/ /pubmed/30764514 http://dx.doi.org/10.3390/jcm8020187 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Wun-Ke
Chen, Chien-An
Chi, Chih-Wen
Li, Li-Hui
Lin, Chin-Ping
Shieh, Hui-Ru
Hsu, Ming-Ling
Ko, Chun-Chuan
Hwang, Jeng-Jong
Chen, Yu-Jen
Moscatilin Inhibits Growth of Human Esophageal Cancer Xenograft and Sensitizes Cancer Cells to Radiotherapy
title Moscatilin Inhibits Growth of Human Esophageal Cancer Xenograft and Sensitizes Cancer Cells to Radiotherapy
title_full Moscatilin Inhibits Growth of Human Esophageal Cancer Xenograft and Sensitizes Cancer Cells to Radiotherapy
title_fullStr Moscatilin Inhibits Growth of Human Esophageal Cancer Xenograft and Sensitizes Cancer Cells to Radiotherapy
title_full_unstemmed Moscatilin Inhibits Growth of Human Esophageal Cancer Xenograft and Sensitizes Cancer Cells to Radiotherapy
title_short Moscatilin Inhibits Growth of Human Esophageal Cancer Xenograft and Sensitizes Cancer Cells to Radiotherapy
title_sort moscatilin inhibits growth of human esophageal cancer xenograft and sensitizes cancer cells to radiotherapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406854/
https://www.ncbi.nlm.nih.gov/pubmed/30764514
http://dx.doi.org/10.3390/jcm8020187
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