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Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device

The separation of circulating tumor cells (CTCs) from the peripheral blood is an important issue that has been highlighted because of their high clinical potential. However, techniques that depend solely on tumor-specific surface molecules or just the larger size of CTCs are limited by tumor heterog...

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Detalles Bibliográficos
Autores principales: Yoon, Yousang, Lee, Jusin, Ra, Moonsoo, Gwon, Hyeokshin, Lee, Seungwon, Kim, Min Young, Yoo, Ki-Chun, Sul, Onejae, Kim, Chul Geun, Kim, Whoi-Yul, Park, Jea-Gun, Lee, Su-Jae, Lee, Young Yiul, Choi, Ho Soon, Lee, Seung-Beck
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406949/
https://www.ncbi.nlm.nih.gov/pubmed/30744156
http://dx.doi.org/10.3390/cancers11020200
Descripción
Sumario:The separation of circulating tumor cells (CTCs) from the peripheral blood is an important issue that has been highlighted because of their high clinical potential. However, techniques that depend solely on tumor-specific surface molecules or just the larger size of CTCs are limited by tumor heterogeneity. Here, we present a slanted weir microfluidic device that utilizes the size and deformability of CTCs to separate them from the unprocessed whole blood. By testing its ability using a highly invasive breast cancer cell line, our device achieved a 97% separation efficiency, while showing an 8-log depletion of erythrocytes and 5.6-log depletion of leukocytes. We also developed an image analysis tool that was able to characterize the various morphologies and differing deformability of the separating cells. From the results, we believe our system possesses a high potential for liquid biopsy, aiding future cancer research.