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Data Independent Acquisition Mass Spectrometry Can Identify Circulating Proteins That Predict Future Weight Loss with a Diet and Exercise Programme

We investigated biological determinants that would associate with the response to a diet and weight loss programme in impaired glucose regulation (IGR) people using sequential window acquisition of all theoretical fragment ion spectra (SWATH) mass spectrometry (MS), a data acquisition method which c...

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Autores principales: Malipatil, Nagaraj, Fachim, Helene A., Siddals, Kirk, Geary, Bethany, Wark, Gwen, Porter, Nick, Anderson, Simon, Donn, Rachelle, Harvie, Michelle, Whetton, Anthony D., Gibson, Martin J., Heald, Adrian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406968/
https://www.ncbi.nlm.nih.gov/pubmed/30691056
http://dx.doi.org/10.3390/jcm8020141
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author Malipatil, Nagaraj
Fachim, Helene A.
Siddals, Kirk
Geary, Bethany
Wark, Gwen
Porter, Nick
Anderson, Simon
Donn, Rachelle
Harvie, Michelle
Whetton, Anthony D.
Gibson, Martin J.
Heald, Adrian
author_facet Malipatil, Nagaraj
Fachim, Helene A.
Siddals, Kirk
Geary, Bethany
Wark, Gwen
Porter, Nick
Anderson, Simon
Donn, Rachelle
Harvie, Michelle
Whetton, Anthony D.
Gibson, Martin J.
Heald, Adrian
author_sort Malipatil, Nagaraj
collection PubMed
description We investigated biological determinants that would associate with the response to a diet and weight loss programme in impaired glucose regulation (IGR) people using sequential window acquisition of all theoretical fragment ion spectra (SWATH) mass spectrometry (MS), a data acquisition method which complement traditional mass spectrometry-based proteomics techniques. Ten women and 10 men with IGR underwent anthropometric measurements and fasting blood tests. SWATH MS was carried out with subsequent immunoassay of specific peptide levels. After a six-month intervention, 40% of participants lost 3% or more in weight, 45% of patients remained within 3% of their starting weight and 15% increased their weight by 3% or more. Hemoglobin A1c (HbA1C) level was reduced with weight loss with improvements in insulin sensitivity. SWATH MS on pre-intervention samples and subsequent principal component analysis identified a cluster of proteins associated with future weight loss, including insulin-like growth factor-II (IGF-II) and Vitamin D binding protein. Individuals who lost 3% in weight had significantly higher baseline IGF-II levels than those who did not lose weight. SWATH MS successfully discriminated between individuals who were more likely to lose weight and potentially improve their sensitivity to insulin. A higher IGF-II baseline was predictive of success with weight reduction, suggesting that biological determinants are important in response to weight loss and exercise regimes. This may permit better targeting of interventions to prevent diabetes in the future.
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spelling pubmed-64069682019-03-22 Data Independent Acquisition Mass Spectrometry Can Identify Circulating Proteins That Predict Future Weight Loss with a Diet and Exercise Programme Malipatil, Nagaraj Fachim, Helene A. Siddals, Kirk Geary, Bethany Wark, Gwen Porter, Nick Anderson, Simon Donn, Rachelle Harvie, Michelle Whetton, Anthony D. Gibson, Martin J. Heald, Adrian J Clin Med Article We investigated biological determinants that would associate with the response to a diet and weight loss programme in impaired glucose regulation (IGR) people using sequential window acquisition of all theoretical fragment ion spectra (SWATH) mass spectrometry (MS), a data acquisition method which complement traditional mass spectrometry-based proteomics techniques. Ten women and 10 men with IGR underwent anthropometric measurements and fasting blood tests. SWATH MS was carried out with subsequent immunoassay of specific peptide levels. After a six-month intervention, 40% of participants lost 3% or more in weight, 45% of patients remained within 3% of their starting weight and 15% increased their weight by 3% or more. Hemoglobin A1c (HbA1C) level was reduced with weight loss with improvements in insulin sensitivity. SWATH MS on pre-intervention samples and subsequent principal component analysis identified a cluster of proteins associated with future weight loss, including insulin-like growth factor-II (IGF-II) and Vitamin D binding protein. Individuals who lost 3% in weight had significantly higher baseline IGF-II levels than those who did not lose weight. SWATH MS successfully discriminated between individuals who were more likely to lose weight and potentially improve their sensitivity to insulin. A higher IGF-II baseline was predictive of success with weight reduction, suggesting that biological determinants are important in response to weight loss and exercise regimes. This may permit better targeting of interventions to prevent diabetes in the future. MDPI 2019-01-25 /pmc/articles/PMC6406968/ /pubmed/30691056 http://dx.doi.org/10.3390/jcm8020141 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Malipatil, Nagaraj
Fachim, Helene A.
Siddals, Kirk
Geary, Bethany
Wark, Gwen
Porter, Nick
Anderson, Simon
Donn, Rachelle
Harvie, Michelle
Whetton, Anthony D.
Gibson, Martin J.
Heald, Adrian
Data Independent Acquisition Mass Spectrometry Can Identify Circulating Proteins That Predict Future Weight Loss with a Diet and Exercise Programme
title Data Independent Acquisition Mass Spectrometry Can Identify Circulating Proteins That Predict Future Weight Loss with a Diet and Exercise Programme
title_full Data Independent Acquisition Mass Spectrometry Can Identify Circulating Proteins That Predict Future Weight Loss with a Diet and Exercise Programme
title_fullStr Data Independent Acquisition Mass Spectrometry Can Identify Circulating Proteins That Predict Future Weight Loss with a Diet and Exercise Programme
title_full_unstemmed Data Independent Acquisition Mass Spectrometry Can Identify Circulating Proteins That Predict Future Weight Loss with a Diet and Exercise Programme
title_short Data Independent Acquisition Mass Spectrometry Can Identify Circulating Proteins That Predict Future Weight Loss with a Diet and Exercise Programme
title_sort data independent acquisition mass spectrometry can identify circulating proteins that predict future weight loss with a diet and exercise programme
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406968/
https://www.ncbi.nlm.nih.gov/pubmed/30691056
http://dx.doi.org/10.3390/jcm8020141
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