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Fecal Microbiota Analysis in Patients Going through a Depressive Episode during Treatment in a Psychiatric Hospital Setting

Rationale: There is a worldwide prevalence of generalized anxiety and major depressive disorders (MDD). Gut–brain axis dysfunction, antibacterial activity, and modulatory effects of antidepressants toward intestinal bacteria have been shown both in vitro and in vivo. Objectives: In this study, we ai...

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Autores principales: Liśkiewicz, Paweł, Pełka-Wysiecka, Justyna, Kaczmarczyk, Mariusz, Łoniewski, Igor, Wroński, Michał, Bąba-Kubiś, Agata, Skonieczna-Żydecka, Karolina, Marlicz, Wojciech, Misiak, Błażej, Samochowiec, Jerzy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407012/
https://www.ncbi.nlm.nih.gov/pubmed/30717162
http://dx.doi.org/10.3390/jcm8020164
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author Liśkiewicz, Paweł
Pełka-Wysiecka, Justyna
Kaczmarczyk, Mariusz
Łoniewski, Igor
Wroński, Michał
Bąba-Kubiś, Agata
Skonieczna-Żydecka, Karolina
Marlicz, Wojciech
Misiak, Błażej
Samochowiec, Jerzy
author_facet Liśkiewicz, Paweł
Pełka-Wysiecka, Justyna
Kaczmarczyk, Mariusz
Łoniewski, Igor
Wroński, Michał
Bąba-Kubiś, Agata
Skonieczna-Żydecka, Karolina
Marlicz, Wojciech
Misiak, Błażej
Samochowiec, Jerzy
author_sort Liśkiewicz, Paweł
collection PubMed
description Rationale: There is a worldwide prevalence of generalized anxiety and major depressive disorders (MDD). Gut–brain axis dysfunction, antibacterial activity, and modulatory effects of antidepressants toward intestinal bacteria have been shown both in vitro and in vivo. Objectives: In this study, we aimed to investigate the effects of hospital stay, including escitalopram administration, on gut microbiota in patients with depressive episodes. Methods: After admission to the hospital and 7-days washout from all medications the composition of fecal microbiota samples was evaluated at baseline (W0) and after 6 weeks (W6), using 16S rRNA sequencing. The study was conducted on 17 inpatients (52.9% females), who followed the same daily hospital routine, including a standard diet and received 5–20 mg daily doses of escitalopram. Results: At the end of treatment (W6), no change was observed in the Chao1 index. However, Shannon (median (Q1–Q3): W0 2.78 (2.67–3.02) vs. W6 3.11 (2.80–3.30)), and inverse Simpson (median (Q1–Q3): W0 9.26 (7.26–13.76) vs. W6 12.13 (9.17–15.73)) indices increased significantly compared to baseline values (False Discovery Rate p (q) = 0.031 and q = 0.011, respectively). We also found that between-subject W0 Bray–Curtis dissimilarities were significantly higher than W0–W6 within-subject dissimilarities (median (Q1–Q3): 0.68 (0.56–0.77) vs. 0.38 (0.35–0.52), two sided Mann–Whitney test p < 0.00001. The within-subject dissimilarities did not depend on sex, age, BMI, illness duration and a daily dose of escitalopram. No significant differences between taxa levels, at the studied time points, were observed when adjusted for multiple hypotheses testing procedures. Conclusions: We conclude that a six-week treatment in a psychiatric hospital setting resulted in increased alpha biodiversity in fecal microbiota, however its causal relationship with patients’ mental health was not proved. We have also found that individual microbiome stability was not affected by hospitalization.
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spelling pubmed-64070122019-03-22 Fecal Microbiota Analysis in Patients Going through a Depressive Episode during Treatment in a Psychiatric Hospital Setting Liśkiewicz, Paweł Pełka-Wysiecka, Justyna Kaczmarczyk, Mariusz Łoniewski, Igor Wroński, Michał Bąba-Kubiś, Agata Skonieczna-Żydecka, Karolina Marlicz, Wojciech Misiak, Błażej Samochowiec, Jerzy J Clin Med Article Rationale: There is a worldwide prevalence of generalized anxiety and major depressive disorders (MDD). Gut–brain axis dysfunction, antibacterial activity, and modulatory effects of antidepressants toward intestinal bacteria have been shown both in vitro and in vivo. Objectives: In this study, we aimed to investigate the effects of hospital stay, including escitalopram administration, on gut microbiota in patients with depressive episodes. Methods: After admission to the hospital and 7-days washout from all medications the composition of fecal microbiota samples was evaluated at baseline (W0) and after 6 weeks (W6), using 16S rRNA sequencing. The study was conducted on 17 inpatients (52.9% females), who followed the same daily hospital routine, including a standard diet and received 5–20 mg daily doses of escitalopram. Results: At the end of treatment (W6), no change was observed in the Chao1 index. However, Shannon (median (Q1–Q3): W0 2.78 (2.67–3.02) vs. W6 3.11 (2.80–3.30)), and inverse Simpson (median (Q1–Q3): W0 9.26 (7.26–13.76) vs. W6 12.13 (9.17–15.73)) indices increased significantly compared to baseline values (False Discovery Rate p (q) = 0.031 and q = 0.011, respectively). We also found that between-subject W0 Bray–Curtis dissimilarities were significantly higher than W0–W6 within-subject dissimilarities (median (Q1–Q3): 0.68 (0.56–0.77) vs. 0.38 (0.35–0.52), two sided Mann–Whitney test p < 0.00001. The within-subject dissimilarities did not depend on sex, age, BMI, illness duration and a daily dose of escitalopram. No significant differences between taxa levels, at the studied time points, were observed when adjusted for multiple hypotheses testing procedures. Conclusions: We conclude that a six-week treatment in a psychiatric hospital setting resulted in increased alpha biodiversity in fecal microbiota, however its causal relationship with patients’ mental health was not proved. We have also found that individual microbiome stability was not affected by hospitalization. MDPI 2019-02-01 /pmc/articles/PMC6407012/ /pubmed/30717162 http://dx.doi.org/10.3390/jcm8020164 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liśkiewicz, Paweł
Pełka-Wysiecka, Justyna
Kaczmarczyk, Mariusz
Łoniewski, Igor
Wroński, Michał
Bąba-Kubiś, Agata
Skonieczna-Żydecka, Karolina
Marlicz, Wojciech
Misiak, Błażej
Samochowiec, Jerzy
Fecal Microbiota Analysis in Patients Going through a Depressive Episode during Treatment in a Psychiatric Hospital Setting
title Fecal Microbiota Analysis in Patients Going through a Depressive Episode during Treatment in a Psychiatric Hospital Setting
title_full Fecal Microbiota Analysis in Patients Going through a Depressive Episode during Treatment in a Psychiatric Hospital Setting
title_fullStr Fecal Microbiota Analysis in Patients Going through a Depressive Episode during Treatment in a Psychiatric Hospital Setting
title_full_unstemmed Fecal Microbiota Analysis in Patients Going through a Depressive Episode during Treatment in a Psychiatric Hospital Setting
title_short Fecal Microbiota Analysis in Patients Going through a Depressive Episode during Treatment in a Psychiatric Hospital Setting
title_sort fecal microbiota analysis in patients going through a depressive episode during treatment in a psychiatric hospital setting
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407012/
https://www.ncbi.nlm.nih.gov/pubmed/30717162
http://dx.doi.org/10.3390/jcm8020164
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