Cargando…

Exosome Analysis in Tumor-Draining Pulmonary Vein Identifies NSCLC Patients with Higher Risk of Relapse after Curative Surgery

Since tumor-draining pulmonary vein blood (PV) is enriched in tumor-secreted products, we hypothesized that it would also be enriched in tumor-derived exosomes, which would be important in the metastasis process. We characterized exosomes from PV of 61 resected non-small cell lung cancer (NSCLC) pat...

Descripción completa

Detalles Bibliográficos
Autores principales: Navarro, Alfons, Molins, Laureano, Marrades, Ramon M., Moises, Jorge, Viñolas, Nuria, Morales, Sara, Canals, Jordi, Castellano, Joan J., Ramírez, José, Monzo, Mariano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407158/
https://www.ncbi.nlm.nih.gov/pubmed/30795562
http://dx.doi.org/10.3390/cancers11020249
_version_ 1783401483871453184
author Navarro, Alfons
Molins, Laureano
Marrades, Ramon M.
Moises, Jorge
Viñolas, Nuria
Morales, Sara
Canals, Jordi
Castellano, Joan J.
Ramírez, José
Monzo, Mariano
author_facet Navarro, Alfons
Molins, Laureano
Marrades, Ramon M.
Moises, Jorge
Viñolas, Nuria
Morales, Sara
Canals, Jordi
Castellano, Joan J.
Ramírez, José
Monzo, Mariano
author_sort Navarro, Alfons
collection PubMed
description Since tumor-draining pulmonary vein blood (PV) is enriched in tumor-secreted products, we hypothesized that it would also be enriched in tumor-derived exosomes, which would be important in the metastasis process. We characterized exosomes from PV of 61 resected non-small cell lung cancer (NSCLC) patients to evaluate its potential as relapse biomarkers. Exosomes were characterized using transmission electron microscopy, western blot and nanoparticle tracking analysis and we examined time to relapse (TTR) and overall survival (OS). Differences between PV and peripheral vein were found. PV was enriched in smaller exosomes than the paired peripheral vein (p = 0.01). Moreover, PV exosome size mode was able to identify relapsed patients (Area under the curve [AUC] = 0.781; 95%CI: 0.6641–0.8978), in whom exosome size was smaller (<112 nm; p < 0.001). The combination of PV exosome size and N (lymph node involvement) showed an AUC of 0.89 (95%CI: 0.80–0.97). Moreover, smaller PV exosome size was associated with shorter TTR (28.3 months vs. not reached, p < 0.001) and OS (43.9 months vs. not reached, p = 0.009). Multivariate analyses identified PV exosome size and stage as independent prognostic markers for TTR and OS. PV exosome size is a promising relapse biomarker after surgery that can add valuable information to clinical variables.
format Online
Article
Text
id pubmed-6407158
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64071582019-03-21 Exosome Analysis in Tumor-Draining Pulmonary Vein Identifies NSCLC Patients with Higher Risk of Relapse after Curative Surgery Navarro, Alfons Molins, Laureano Marrades, Ramon M. Moises, Jorge Viñolas, Nuria Morales, Sara Canals, Jordi Castellano, Joan J. Ramírez, José Monzo, Mariano Cancers (Basel) Article Since tumor-draining pulmonary vein blood (PV) is enriched in tumor-secreted products, we hypothesized that it would also be enriched in tumor-derived exosomes, which would be important in the metastasis process. We characterized exosomes from PV of 61 resected non-small cell lung cancer (NSCLC) patients to evaluate its potential as relapse biomarkers. Exosomes were characterized using transmission electron microscopy, western blot and nanoparticle tracking analysis and we examined time to relapse (TTR) and overall survival (OS). Differences between PV and peripheral vein were found. PV was enriched in smaller exosomes than the paired peripheral vein (p = 0.01). Moreover, PV exosome size mode was able to identify relapsed patients (Area under the curve [AUC] = 0.781; 95%CI: 0.6641–0.8978), in whom exosome size was smaller (<112 nm; p < 0.001). The combination of PV exosome size and N (lymph node involvement) showed an AUC of 0.89 (95%CI: 0.80–0.97). Moreover, smaller PV exosome size was associated with shorter TTR (28.3 months vs. not reached, p < 0.001) and OS (43.9 months vs. not reached, p = 0.009). Multivariate analyses identified PV exosome size and stage as independent prognostic markers for TTR and OS. PV exosome size is a promising relapse biomarker after surgery that can add valuable information to clinical variables. MDPI 2019-02-21 /pmc/articles/PMC6407158/ /pubmed/30795562 http://dx.doi.org/10.3390/cancers11020249 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Navarro, Alfons
Molins, Laureano
Marrades, Ramon M.
Moises, Jorge
Viñolas, Nuria
Morales, Sara
Canals, Jordi
Castellano, Joan J.
Ramírez, José
Monzo, Mariano
Exosome Analysis in Tumor-Draining Pulmonary Vein Identifies NSCLC Patients with Higher Risk of Relapse after Curative Surgery
title Exosome Analysis in Tumor-Draining Pulmonary Vein Identifies NSCLC Patients with Higher Risk of Relapse after Curative Surgery
title_full Exosome Analysis in Tumor-Draining Pulmonary Vein Identifies NSCLC Patients with Higher Risk of Relapse after Curative Surgery
title_fullStr Exosome Analysis in Tumor-Draining Pulmonary Vein Identifies NSCLC Patients with Higher Risk of Relapse after Curative Surgery
title_full_unstemmed Exosome Analysis in Tumor-Draining Pulmonary Vein Identifies NSCLC Patients with Higher Risk of Relapse after Curative Surgery
title_short Exosome Analysis in Tumor-Draining Pulmonary Vein Identifies NSCLC Patients with Higher Risk of Relapse after Curative Surgery
title_sort exosome analysis in tumor-draining pulmonary vein identifies nsclc patients with higher risk of relapse after curative surgery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407158/
https://www.ncbi.nlm.nih.gov/pubmed/30795562
http://dx.doi.org/10.3390/cancers11020249
work_keys_str_mv AT navarroalfons exosomeanalysisintumordrainingpulmonaryveinidentifiesnsclcpatientswithhigherriskofrelapseaftercurativesurgery
AT molinslaureano exosomeanalysisintumordrainingpulmonaryveinidentifiesnsclcpatientswithhigherriskofrelapseaftercurativesurgery
AT marradesramonm exosomeanalysisintumordrainingpulmonaryveinidentifiesnsclcpatientswithhigherriskofrelapseaftercurativesurgery
AT moisesjorge exosomeanalysisintumordrainingpulmonaryveinidentifiesnsclcpatientswithhigherriskofrelapseaftercurativesurgery
AT vinolasnuria exosomeanalysisintumordrainingpulmonaryveinidentifiesnsclcpatientswithhigherriskofrelapseaftercurativesurgery
AT moralessara exosomeanalysisintumordrainingpulmonaryveinidentifiesnsclcpatientswithhigherriskofrelapseaftercurativesurgery
AT canalsjordi exosomeanalysisintumordrainingpulmonaryveinidentifiesnsclcpatientswithhigherriskofrelapseaftercurativesurgery
AT castellanojoanj exosomeanalysisintumordrainingpulmonaryveinidentifiesnsclcpatientswithhigherriskofrelapseaftercurativesurgery
AT ramirezjose exosomeanalysisintumordrainingpulmonaryveinidentifiesnsclcpatientswithhigherriskofrelapseaftercurativesurgery
AT monzomariano exosomeanalysisintumordrainingpulmonaryveinidentifiesnsclcpatientswithhigherriskofrelapseaftercurativesurgery