Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis

South Africa remains challenged with a high tuberculosis burden accompanied by an increase in drug resistant cases. We assessed the use of the Illumina MiSeq, a next-generation sequencing platform for whole genome sequencing, followed by bioinformatic analysis using a commercial software package to...

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Autores principales: Omar, Shaheed V., Joseph, Lavania, Said, Halima M., Ismail, Farzana, Ismail, Nabila, Gwala, Thabisile L., Ismail, Nazir A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AOSIS 2019
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407317/
https://www.ncbi.nlm.nih.gov/pubmed/30863717
http://dx.doi.org/10.4102/ajlm.v8i1.801
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author Omar, Shaheed V.
Joseph, Lavania
Said, Halima M.
Ismail, Farzana
Ismail, Nabila
Gwala, Thabisile L.
Ismail, Nazir A.
author_facet Omar, Shaheed V.
Joseph, Lavania
Said, Halima M.
Ismail, Farzana
Ismail, Nabila
Gwala, Thabisile L.
Ismail, Nazir A.
author_sort Omar, Shaheed V.
collection PubMed
description South Africa remains challenged with a high tuberculosis burden accompanied by an increase in drug resistant cases. We assessed the use of the Illumina MiSeq, a next-generation sequencing platform for whole genome sequencing, followed by bioinformatic analysis using a commercial software package to determine resistance to selected drugs used for Mycobacterium tuberculosis treatment in our setting. Whole genome sequencing shows potential as a diagnostic platform for the detection of drug resistance in Mycobacterium tuberculosis with the provision of information for several drugs simultaneously.
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spelling pubmed-64073172019-03-12 Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis Omar, Shaheed V. Joseph, Lavania Said, Halima M. Ismail, Farzana Ismail, Nabila Gwala, Thabisile L. Ismail, Nazir A. Afr J Lab Med Brief Report South Africa remains challenged with a high tuberculosis burden accompanied by an increase in drug resistant cases. We assessed the use of the Illumina MiSeq, a next-generation sequencing platform for whole genome sequencing, followed by bioinformatic analysis using a commercial software package to determine resistance to selected drugs used for Mycobacterium tuberculosis treatment in our setting. Whole genome sequencing shows potential as a diagnostic platform for the detection of drug resistance in Mycobacterium tuberculosis with the provision of information for several drugs simultaneously. AOSIS 2019-02-21 /pmc/articles/PMC6407317/ /pubmed/30863717 http://dx.doi.org/10.4102/ajlm.v8i1.801 Text en © 2019. The Authors https://creativecommons.org/licenses/by/4.0/ Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.
spellingShingle Brief Report
Omar, Shaheed V.
Joseph, Lavania
Said, Halima M.
Ismail, Farzana
Ismail, Nabila
Gwala, Thabisile L.
Ismail, Nazir A.
Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis
title Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis
title_full Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis
title_fullStr Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis
title_full_unstemmed Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis
title_short Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis
title_sort whole genome sequencing for drug resistance determination in mycobacterium tuberculosis
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407317/
https://www.ncbi.nlm.nih.gov/pubmed/30863717
http://dx.doi.org/10.4102/ajlm.v8i1.801
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