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A thermosensitive gel based on w1/o/w2 multiple microemulsions for the vaginal delivery of small nucleic acid
The present study aims at designing a thermosensitive gel prepared from w1/o/w2 multiple microemulsions (MMEs) for the vaginal delivery of siRNA. The w1/o/w2 MMEs were prepared by two-step emulsifications: the first step was to prepare primary emulsions (w1/o) by low energy emulsification (LEE); the...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407577/ https://www.ncbi.nlm.nih.gov/pubmed/30822166 http://dx.doi.org/10.1080/10717544.2019.1568622 |
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author | Wang, Jiu Wang, Yajing Wang, Ziqiang Wang, Fan He, Jie Yang, Xiaoyun Xie, Weidong Liu, Ying Zhang, Yaou |
author_facet | Wang, Jiu Wang, Yajing Wang, Ziqiang Wang, Fan He, Jie Yang, Xiaoyun Xie, Weidong Liu, Ying Zhang, Yaou |
author_sort | Wang, Jiu |
collection | PubMed |
description | The present study aims at designing a thermosensitive gel prepared from w1/o/w2 multiple microemulsions (MMEs) for the vaginal delivery of siRNA. The w1/o/w2 MMEs were prepared by two-step emulsifications: the first step was to prepare primary emulsions (w1/o) by low energy emulsification (LEE); the second step was to obtain stable w1/o/w2 MMEs by self-emulsifying. An extensive formulation optimization process was undertaken. The final w1/o/w2 MMEs could be formed in ddH(2)O, phosphate buffer solution (PBS, pH 7.4) and 1640 culture media with diameter size about 166.5 ± 13.1, 271.0 ± 11.1 and 278.7 ± 12.1 nm respectively. The release rates of siRNA from solutions, MMEs and MMEs-gels were completed within 2 h, 6 h and13 h respectively. The transfection efficiency of MMEs was confirmed both in vitro and in vivo. The relative target gene expressions of MMEs were 0.07 ± 0.05% vs. 0.37 ± 0.06% in Hela cells against Lipofectamine2000® and 1.88% ± 0.00% vs. 9.65% ± 0.02% in mouse vaginal mucosa against PEI. Good biocompatibility of MMEs was verified by cytotoxicity and pathological studies. Overall, our results indicated the potential of the MMEs-gel system for the vaginal delivery of siRNA. |
format | Online Article Text |
id | pubmed-6407577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-64075772019-03-12 A thermosensitive gel based on w1/o/w2 multiple microemulsions for the vaginal delivery of small nucleic acid Wang, Jiu Wang, Yajing Wang, Ziqiang Wang, Fan He, Jie Yang, Xiaoyun Xie, Weidong Liu, Ying Zhang, Yaou Drug Deliv Research Article The present study aims at designing a thermosensitive gel prepared from w1/o/w2 multiple microemulsions (MMEs) for the vaginal delivery of siRNA. The w1/o/w2 MMEs were prepared by two-step emulsifications: the first step was to prepare primary emulsions (w1/o) by low energy emulsification (LEE); the second step was to obtain stable w1/o/w2 MMEs by self-emulsifying. An extensive formulation optimization process was undertaken. The final w1/o/w2 MMEs could be formed in ddH(2)O, phosphate buffer solution (PBS, pH 7.4) and 1640 culture media with diameter size about 166.5 ± 13.1, 271.0 ± 11.1 and 278.7 ± 12.1 nm respectively. The release rates of siRNA from solutions, MMEs and MMEs-gels were completed within 2 h, 6 h and13 h respectively. The transfection efficiency of MMEs was confirmed both in vitro and in vivo. The relative target gene expressions of MMEs were 0.07 ± 0.05% vs. 0.37 ± 0.06% in Hela cells against Lipofectamine2000® and 1.88% ± 0.00% vs. 9.65% ± 0.02% in mouse vaginal mucosa against PEI. Good biocompatibility of MMEs was verified by cytotoxicity and pathological studies. Overall, our results indicated the potential of the MMEs-gel system for the vaginal delivery of siRNA. Taylor & Francis 2019-03-01 /pmc/articles/PMC6407577/ /pubmed/30822166 http://dx.doi.org/10.1080/10717544.2019.1568622 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Jiu Wang, Yajing Wang, Ziqiang Wang, Fan He, Jie Yang, Xiaoyun Xie, Weidong Liu, Ying Zhang, Yaou A thermosensitive gel based on w1/o/w2 multiple microemulsions for the vaginal delivery of small nucleic acid |
title | A thermosensitive gel based on w1/o/w2 multiple microemulsions for the vaginal delivery of small nucleic acid |
title_full | A thermosensitive gel based on w1/o/w2 multiple microemulsions for the vaginal delivery of small nucleic acid |
title_fullStr | A thermosensitive gel based on w1/o/w2 multiple microemulsions for the vaginal delivery of small nucleic acid |
title_full_unstemmed | A thermosensitive gel based on w1/o/w2 multiple microemulsions for the vaginal delivery of small nucleic acid |
title_short | A thermosensitive gel based on w1/o/w2 multiple microemulsions for the vaginal delivery of small nucleic acid |
title_sort | thermosensitive gel based on w1/o/w2 multiple microemulsions for the vaginal delivery of small nucleic acid |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407577/ https://www.ncbi.nlm.nih.gov/pubmed/30822166 http://dx.doi.org/10.1080/10717544.2019.1568622 |
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