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Association of increased B7 protein expression by infiltrating immune cells with progression of gastric carcinogenesis

B7 negative costimulatory molecules are a group of molecules associated with the occurrence, development, and therapy of cancers. Here, we aimed to determine the clinical significance of PD-L1, B7-H3, and B7-H4 and their expression in CD8 and CD68 positive cells at different stages of gastric carcin...

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Autores principales: Guo, Lingchuan, Liu, Zhiju, Zhang, Yun, Quan, Qiuying, Huang, Lili, Xu, Yunyun, Cao, Lei, Zhang, Xueguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407991/
https://www.ncbi.nlm.nih.gov/pubmed/30813210
http://dx.doi.org/10.1097/MD.0000000000014663
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author Guo, Lingchuan
Liu, Zhiju
Zhang, Yun
Quan, Qiuying
Huang, Lili
Xu, Yunyun
Cao, Lei
Zhang, Xueguang
author_facet Guo, Lingchuan
Liu, Zhiju
Zhang, Yun
Quan, Qiuying
Huang, Lili
Xu, Yunyun
Cao, Lei
Zhang, Xueguang
author_sort Guo, Lingchuan
collection PubMed
description B7 negative costimulatory molecules are a group of molecules associated with the occurrence, development, and therapy of cancers. Here, we aimed to determine the clinical significance of PD-L1, B7-H3, and B7-H4 and their expression in CD8 and CD68 positive cells at different stages of gastric carcinogenesis. We detected PD-L1, B7-H3, B7-H4, CD8, and CD68 expression in samples by immunohistochemical staining of 62 chronic superficial gastritis (CSG) samples, 72 chronic atrophic gastritis (CAG) samples, 68 low-grade intraepithelial neoplasia (LIN) samples, 65 high-grade intraepithelial neoplasia (HIN) samples obtained from gastroscopic biopsies and 50 gastric adenocarcinoma (GA) samples obtained from surgical resections. Then we statistically analyzed the expression differences and correlations. Our results indicated that B7 and CD68 expression on infiltrating immune cells was associated with disease progression. However, infiltration of CD8+ cells decreased with disease progression. B7-H3 expression was markedly enhanced at neoplasia and GA stages. B7-H3 in tumor cells was negatively correlated with CD8-expressing cells. Conversely, B7-H3 expression in tumor-infiltrating immune cells was positively correlated with CD68-expressing cells. B7-H4 expression was found in the cell membrane at the stages of gastritis and low-grade neoplasia and was gradually expressed in the cytoplasm at high-grade neoplasia and GA stages. High B7-H4 expression in infiltrating immune cells was also significantly associated with lower CD8-positive and higher CD68-positive cell densities. Increased B7 protein expression by infiltrating immune cells was associated with disease progression, and specifically, the level of B7-H3 expression and localization of B7-H4 expression differed significantly among different stages of gastric carcinogenesis.
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spelling pubmed-64079912019-03-16 Association of increased B7 protein expression by infiltrating immune cells with progression of gastric carcinogenesis Guo, Lingchuan Liu, Zhiju Zhang, Yun Quan, Qiuying Huang, Lili Xu, Yunyun Cao, Lei Zhang, Xueguang Medicine (Baltimore) Research Article B7 negative costimulatory molecules are a group of molecules associated with the occurrence, development, and therapy of cancers. Here, we aimed to determine the clinical significance of PD-L1, B7-H3, and B7-H4 and their expression in CD8 and CD68 positive cells at different stages of gastric carcinogenesis. We detected PD-L1, B7-H3, B7-H4, CD8, and CD68 expression in samples by immunohistochemical staining of 62 chronic superficial gastritis (CSG) samples, 72 chronic atrophic gastritis (CAG) samples, 68 low-grade intraepithelial neoplasia (LIN) samples, 65 high-grade intraepithelial neoplasia (HIN) samples obtained from gastroscopic biopsies and 50 gastric adenocarcinoma (GA) samples obtained from surgical resections. Then we statistically analyzed the expression differences and correlations. Our results indicated that B7 and CD68 expression on infiltrating immune cells was associated with disease progression. However, infiltration of CD8+ cells decreased with disease progression. B7-H3 expression was markedly enhanced at neoplasia and GA stages. B7-H3 in tumor cells was negatively correlated with CD8-expressing cells. Conversely, B7-H3 expression in tumor-infiltrating immune cells was positively correlated with CD68-expressing cells. B7-H4 expression was found in the cell membrane at the stages of gastritis and low-grade neoplasia and was gradually expressed in the cytoplasm at high-grade neoplasia and GA stages. High B7-H4 expression in infiltrating immune cells was also significantly associated with lower CD8-positive and higher CD68-positive cell densities. Increased B7 protein expression by infiltrating immune cells was associated with disease progression, and specifically, the level of B7-H3 expression and localization of B7-H4 expression differed significantly among different stages of gastric carcinogenesis. Wolters Kluwer Health 2019-02-22 /pmc/articles/PMC6407991/ /pubmed/30813210 http://dx.doi.org/10.1097/MD.0000000000014663 Text en Copyright © 2019 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0
spellingShingle Research Article
Guo, Lingchuan
Liu, Zhiju
Zhang, Yun
Quan, Qiuying
Huang, Lili
Xu, Yunyun
Cao, Lei
Zhang, Xueguang
Association of increased B7 protein expression by infiltrating immune cells with progression of gastric carcinogenesis
title Association of increased B7 protein expression by infiltrating immune cells with progression of gastric carcinogenesis
title_full Association of increased B7 protein expression by infiltrating immune cells with progression of gastric carcinogenesis
title_fullStr Association of increased B7 protein expression by infiltrating immune cells with progression of gastric carcinogenesis
title_full_unstemmed Association of increased B7 protein expression by infiltrating immune cells with progression of gastric carcinogenesis
title_short Association of increased B7 protein expression by infiltrating immune cells with progression of gastric carcinogenesis
title_sort association of increased b7 protein expression by infiltrating immune cells with progression of gastric carcinogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407991/
https://www.ncbi.nlm.nih.gov/pubmed/30813210
http://dx.doi.org/10.1097/MD.0000000000014663
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