Cargando…

Selection and Characterization of Artificial Proteins Targeting the Tubulin α Subunit

Microtubules are cytoskeletal filaments of eukaryotic cells made of αβ-tubulin heterodimers. Structural studies of non-microtubular tubulin rely mainly on molecules that prevent its self-assembly and are used as crystallization chaperones. Here we identified artificial proteins from an αRep library...

Descripción completa

Detalles Bibliográficos
Autores principales:	Campanacci, Valérie, Urvoas, Agathe, Consolati, Tanja, Cantos-Fernandes, Soraya, Aumont-Nicaise, Magali, Valerio-Lepiniec, Marie, Surrey, Thomas, Minard, Philippe, Gigant, Benoît
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cell Press 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408325/ https://www.ncbi.nlm.nih.gov/pubmed/30661854 http://dx.doi.org/10.1016/j.str.2018.12.001

Ejemplares similares

Structural convergence for tubulin binding of CPAP and vinca domain microtubule inhibitors
por: Campanacci, Valérie, et al.
Publicado: (2022)

Specific GFP-binding artificial proteins (αRep): a new tool for in vitro to live cell applications
por: Chevrel, Anne, et al.
Publicado: (2015)

Ligand-induced conformational switch in an artificial bidomain protein scaffold
por: Léger, Corentin, et al.
Publicado: (2019)

The Mechanism of Tubulin Assembly into Microtubules: Insights from Structural Studies
por: Knossow, Marcel, et al.
Publicado: (2020)

Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin
por: Ahmad, Shoeb, et al.
Publicado: (2016)

Insight into microtubule disassembly by kinesin-13s from the structure of Kif2C bound to tubulin
por: Wang, Weiyi, et al.
Publicado: (2017)

Design of an artificial phage-display library based on a new scaffold improved for average stability of the randomized proteins
por: Gomes, M., et al.
Publicado: (2023)

Selection of Specific Protein Binders for Pre-Defined Targets from an Optimized Library of Artificial Helicoidal Repeat Proteins (alphaRep)
por: Guellouz, Asma, et al.
Publicado: (2013)

The structural switch of nucleotide-free kinesin
por: Cao, Luyan, et al.
Publicado: (2017)

Antiviral activity of recombinant ankyrin targeted to the capsid domain of HIV-1 Gag polyprotein
por: Nangola, Sawitree, et al.
Publicado: (2012)

Alpha-helicoidal HEAT-like Repeat Proteins (αRep) Selected as Interactors of HIV-1 Nucleocapsid Negatively Interfere with Viral Genome Packaging and Virus Maturation
por: Hadpech, Sudarat, et al.
Publicado: (2017)

Design, synthesis, and characterization of protein origami based on self-assembly of a brick and staple artificial protein pair
por: Moreaud, Laureen, et al.
Publicado: (2023)

Structural Analysis of the Hanks-Type Protein Kinase YabT From Bacillus subtilis Provides New Insights in its DNA-Dependent Activation
por: Shi, Lei, et al.
Publicado: (2019)

Structural characterization of the RH1-LZI tandem of JIP3/4 highlights RH1 domains as a cytoskeletal motor-binding motif
por: Vilela, Fernando, et al.
Publicado: (2019)

Biophysical characterization data of the artificial protein Octarellin V.1 and binding test with its X-ray helpers
por: Figueroa, Maximiliano, et al.
Publicado: (2016)

Quantifying the Monomer–Dimer Equilibrium of Tubulin with Mass Photometry
por: Fineberg, Adam, et al.
Publicado: (2020)

Heterogeneity of the alpha subunit of tubulin and the variability of tubulin within a single organism
Publicado: (1976)

Microtubule aging probed by microfluidics-assisted tubulin washout
por: Duellberg, Christian, et al.
Publicado: (2016)

GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation
por: Ayukawa, Rie, et al.
Publicado: (2021)

Characterization and Reconstitution of Drosophila γ-Tubulin Ring Complex Subunits
por: Gunawardane, Ruwanthi N., et al.
Publicado: (2000)

Photocatalytic Hydrogen Production and Carbon Dioxide Reduction Catalyzed by an Artificial Cobalt Hemoprotein
por: Udry, Guillermo A. Oliveira, et al.
Publicado: (2022)

Single-cytosine methylation at W-boxes repels binding of WRKY transcription factors through steric hindrance
por: Charvin, Magali, et al.
Publicado: (2023)

Biosynthetic proteins targeting the SARS-CoV-2 spike as anti-virals
por: Thébault, Stéphanie, et al.
Publicado: (2022)

Biophysical and structural characterization of a zinc-responsive repressor of the MarR superfamily
por: Varela, Paloma Fernández, et al.
Publicado: (2019)

Tubulin Subunits Exist in an Activated Conformational State Generated and Maintained by Protein Cofactors
por: Tian, Guoling, et al.
Publicado: (1997)

Structural basis for high specificity of octopine binding in the plant pathogen Agrobacterium tumefaciens
por: Vigouroux, Armelle, et al.
Publicado: (2017)

CLIP-170 tracks growing microtubule ends by dynamically recognizing composite EB1/tubulin-binding sites
por: Bieling, Peter, et al.
Publicado: (2008)

Tubulin isotype regulation maintains asymmetric requirement for α-tubulin over β-tubulin
por: Wethekam, Linnea C., et al.
Publicado: (2023)

In vivo coassembly of a divergent beta-tubulin subunit (c beta 6) into microtubules of different function
Publicado: (1987)

Structure and Functional Analysis of the RNA- and Viral Phosphoprotein-Binding Domain of Respiratory Syncytial Virus M2-1 Protein
por: Blondot, Marie-Lise, et al.
Publicado: (2012)

Physiological regulation of total tubulin and polymerized tubulin in tissues
Publicado: (1977)

Structural basis of tubulin tyrosination by tubulin tyrosine ligase
por: Prota, Andrea E., et al.
Publicado: (2013)

The Tubulin Code and Tubulin-Modifying Enzymes in Autophagy and Cancer
por: Trisciuoglio, Daniela, et al.
Publicado: (2021)

Agrobacterium Uses a Unique Ligand-Binding Mode for Trapping Opines and Acquiring A Competitive Advantage in the Niche Construction on Plant Host
por: Lang, Julien, et al.
Publicado: (2014)

Correction: Structural plasticity of the N-terminal capping helix of the TPR domain of kinesin light chain
por: Nguyen, The Quyen, et al.
Publicado: (2018)

Structural plasticity of the N-terminal capping helix of the TPR domain of kinesin light chain
por: Nguyen, The Quyen, et al.
Publicado: (2017)

Structural Insights of the DciA Helicase Loader in Its Relationship with DNA
por: Cargemel, Claire, et al.
Publicado: (2023)

The discovery of tubulin
por: Wells, William A.
Publicado: (2005)

Tubulin but not microtubule
por: Dove, Alan W.
Publicado: (2002)

The Game of Tubulins
por: Kristensson, Maria Alvarado
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_k14njnbho3sb71rf51nq6gmoe2