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Evolutionary and functional analysis of the plant-specific NADPH oxidase gene family in Brassica rapa L.

NADPH oxidases (NOXs) have been known as respiratory burst oxidase homologues (RBOHs) in plants. To characterize the evolutionary relationships and functions of RBOHs in Brassica rapa, 134 RBOH homologues were identified from 13 plant species, including 14 members (namely BrRBOH01–14) from B. rapa....

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Autores principales: Li, Dahui, Wu, Di, Li, Shizhou, Dai, Yu, Cao, Yunpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408365/
https://www.ncbi.nlm.nih.gov/pubmed/30891283
http://dx.doi.org/10.1098/rsos.181727
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author Li, Dahui
Wu, Di
Li, Shizhou
Dai, Yu
Cao, Yunpeng
author_facet Li, Dahui
Wu, Di
Li, Shizhou
Dai, Yu
Cao, Yunpeng
author_sort Li, Dahui
collection PubMed
description NADPH oxidases (NOXs) have been known as respiratory burst oxidase homologues (RBOHs) in plants. To characterize the evolutionary relationships and functions of RBOHs in Brassica rapa, 134 RBOH homologues were identified from 13 plant species, including 14 members (namely BrRBOH01–14) from B. rapa. There presented 47 gene-pairs among 14 BrRBOHs and other RBOHs, consisting of five pairs within B. rapa, and 15 pairs between B. rapa and Arabidopsis thaliana. Together with phylogenetic analysis, the results suggested that whole-genome duplication might have played an important role in BrRBOH gene expansion, and these duplication events occurred after the divergence of the eudicot and the monocot lineages examined. Furthermore, gene expression of RBOHs in both A. thaliana and B. rapa were assayed via qRT–PCR. An RBOH gene, BrRBOH13 in B. rapa, was transformed into wild-type Arabidopsis plants. The transgenic lines with the overexpressed level of BrRBOH13 conferred to be more tolerant to heavy metal lead (0.05 mM) than wild-type plants. Overall, this integrated analysis at genome-wide level has provided some information on the evolutionary relationships among plant-specific NOXs and the coordinated diversification of gene structure and function in B. rapa.
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spelling pubmed-64083652019-03-19 Evolutionary and functional analysis of the plant-specific NADPH oxidase gene family in Brassica rapa L. Li, Dahui Wu, Di Li, Shizhou Dai, Yu Cao, Yunpeng R Soc Open Sci Biology (Whole Organism) NADPH oxidases (NOXs) have been known as respiratory burst oxidase homologues (RBOHs) in plants. To characterize the evolutionary relationships and functions of RBOHs in Brassica rapa, 134 RBOH homologues were identified from 13 plant species, including 14 members (namely BrRBOH01–14) from B. rapa. There presented 47 gene-pairs among 14 BrRBOHs and other RBOHs, consisting of five pairs within B. rapa, and 15 pairs between B. rapa and Arabidopsis thaliana. Together with phylogenetic analysis, the results suggested that whole-genome duplication might have played an important role in BrRBOH gene expansion, and these duplication events occurred after the divergence of the eudicot and the monocot lineages examined. Furthermore, gene expression of RBOHs in both A. thaliana and B. rapa were assayed via qRT–PCR. An RBOH gene, BrRBOH13 in B. rapa, was transformed into wild-type Arabidopsis plants. The transgenic lines with the overexpressed level of BrRBOH13 conferred to be more tolerant to heavy metal lead (0.05 mM) than wild-type plants. Overall, this integrated analysis at genome-wide level has provided some information on the evolutionary relationships among plant-specific NOXs and the coordinated diversification of gene structure and function in B. rapa. The Royal Society 2019-02-27 /pmc/articles/PMC6408365/ /pubmed/30891283 http://dx.doi.org/10.1098/rsos.181727 Text en © 2019 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Biology (Whole Organism)
Li, Dahui
Wu, Di
Li, Shizhou
Dai, Yu
Cao, Yunpeng
Evolutionary and functional analysis of the plant-specific NADPH oxidase gene family in Brassica rapa L.
title Evolutionary and functional analysis of the plant-specific NADPH oxidase gene family in Brassica rapa L.
title_full Evolutionary and functional analysis of the plant-specific NADPH oxidase gene family in Brassica rapa L.
title_fullStr Evolutionary and functional analysis of the plant-specific NADPH oxidase gene family in Brassica rapa L.
title_full_unstemmed Evolutionary and functional analysis of the plant-specific NADPH oxidase gene family in Brassica rapa L.
title_short Evolutionary and functional analysis of the plant-specific NADPH oxidase gene family in Brassica rapa L.
title_sort evolutionary and functional analysis of the plant-specific nadph oxidase gene family in brassica rapa l.
topic Biology (Whole Organism)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408365/
https://www.ncbi.nlm.nih.gov/pubmed/30891283
http://dx.doi.org/10.1098/rsos.181727
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