Cargando…

Degradation kinetics of cold plasma-treated antibiotics and their antimicrobial activity

Antibiotics, such as ofloxacin (OFX) and ciprofloxacin (CFX), are often detected in considerable concentrations in both wastewater effluents and surface water. This poses a risk to non-target organisms and to human health. The aim of this work was to study atmospheric cold plasma (ACP) degradation o...

Descripción completa

Detalles Bibliográficos
Autores principales: Sarangapani, Chaitanya, Ziuzina, Dana, Behan, Patrice, Boehm, Daniela, Gilmore, Brendan F., Cullen, P. J., Bourke, Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408491/
https://www.ncbi.nlm.nih.gov/pubmed/30850645
http://dx.doi.org/10.1038/s41598-019-40352-9
_version_ 1783401771154014208
author Sarangapani, Chaitanya
Ziuzina, Dana
Behan, Patrice
Boehm, Daniela
Gilmore, Brendan F.
Cullen, P. J.
Bourke, Paula
author_facet Sarangapani, Chaitanya
Ziuzina, Dana
Behan, Patrice
Boehm, Daniela
Gilmore, Brendan F.
Cullen, P. J.
Bourke, Paula
author_sort Sarangapani, Chaitanya
collection PubMed
description Antibiotics, such as ofloxacin (OFX) and ciprofloxacin (CFX), are often detected in considerable concentrations in both wastewater effluents and surface water. This poses a risk to non-target organisms and to human health. The aim of this work was to study atmospheric cold plasma (ACP) degradation of antibiotics in water and meat effluent and to explore any residual antimicrobial activity of samples submitted to the plasma process. The results revealed that ACP successfully degraded the studied antibiotics and that the reaction mechanism is principally related to attack by hydroxyl radicals and ozone. According to the disk diffusion assay, the activity of both antibiotics was considerably reduced by the plasma treatment. However, a microdilution method demonstrated that CFX exhibited higher antimicrobial activity after ACP treatment than the corresponding control revealing a potentially new platform for future research to improve the efficiency of conventional antibiotic treatments. Importantly, short-term exposures to sub-lethal concentrations of the antibiotic equally reduced bacterial susceptibility to both ACP treated and untreated CFX. As a remediation process, ACP removal of antibiotics in complex wastewater effluents is possible. However, it is recommended that plasma encompass degradant structure activity relationships to ensure that biological activity is eliminated against non-target organisms and that life cycle safety of antibiotic compounds is achieved.
format Online
Article
Text
id pubmed-6408491
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-64084912019-03-12 Degradation kinetics of cold plasma-treated antibiotics and their antimicrobial activity Sarangapani, Chaitanya Ziuzina, Dana Behan, Patrice Boehm, Daniela Gilmore, Brendan F. Cullen, P. J. Bourke, Paula Sci Rep Article Antibiotics, such as ofloxacin (OFX) and ciprofloxacin (CFX), are often detected in considerable concentrations in both wastewater effluents and surface water. This poses a risk to non-target organisms and to human health. The aim of this work was to study atmospheric cold plasma (ACP) degradation of antibiotics in water and meat effluent and to explore any residual antimicrobial activity of samples submitted to the plasma process. The results revealed that ACP successfully degraded the studied antibiotics and that the reaction mechanism is principally related to attack by hydroxyl radicals and ozone. According to the disk diffusion assay, the activity of both antibiotics was considerably reduced by the plasma treatment. However, a microdilution method demonstrated that CFX exhibited higher antimicrobial activity after ACP treatment than the corresponding control revealing a potentially new platform for future research to improve the efficiency of conventional antibiotic treatments. Importantly, short-term exposures to sub-lethal concentrations of the antibiotic equally reduced bacterial susceptibility to both ACP treated and untreated CFX. As a remediation process, ACP removal of antibiotics in complex wastewater effluents is possible. However, it is recommended that plasma encompass degradant structure activity relationships to ensure that biological activity is eliminated against non-target organisms and that life cycle safety of antibiotic compounds is achieved. Nature Publishing Group UK 2019-03-08 /pmc/articles/PMC6408491/ /pubmed/30850645 http://dx.doi.org/10.1038/s41598-019-40352-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sarangapani, Chaitanya
Ziuzina, Dana
Behan, Patrice
Boehm, Daniela
Gilmore, Brendan F.
Cullen, P. J.
Bourke, Paula
Degradation kinetics of cold plasma-treated antibiotics and their antimicrobial activity
title Degradation kinetics of cold plasma-treated antibiotics and their antimicrobial activity
title_full Degradation kinetics of cold plasma-treated antibiotics and their antimicrobial activity
title_fullStr Degradation kinetics of cold plasma-treated antibiotics and their antimicrobial activity
title_full_unstemmed Degradation kinetics of cold plasma-treated antibiotics and their antimicrobial activity
title_short Degradation kinetics of cold plasma-treated antibiotics and their antimicrobial activity
title_sort degradation kinetics of cold plasma-treated antibiotics and their antimicrobial activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408491/
https://www.ncbi.nlm.nih.gov/pubmed/30850645
http://dx.doi.org/10.1038/s41598-019-40352-9
work_keys_str_mv AT sarangapanichaitanya degradationkineticsofcoldplasmatreatedantibioticsandtheirantimicrobialactivity
AT ziuzinadana degradationkineticsofcoldplasmatreatedantibioticsandtheirantimicrobialactivity
AT behanpatrice degradationkineticsofcoldplasmatreatedantibioticsandtheirantimicrobialactivity
AT boehmdaniela degradationkineticsofcoldplasmatreatedantibioticsandtheirantimicrobialactivity
AT gilmorebrendanf degradationkineticsofcoldplasmatreatedantibioticsandtheirantimicrobialactivity
AT cullenpj degradationkineticsofcoldplasmatreatedantibioticsandtheirantimicrobialactivity
AT bourkepaula degradationkineticsofcoldplasmatreatedantibioticsandtheirantimicrobialactivity