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Seroprevalence of Human Brucellosis in Wadi Al Dawaser region of Saudi Arabia
BACKGROUND AND OBJECTIVE: Brucellosis is an important zoonotic disease and a major public health problem in the Middle East countries including Saudi Arabia. This study has evaluated the seroprevalence of human brucellosis in Wadi Al Dawaser region of Central Saudi Arabia. METHODS: The study was con...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Professional Medical Publications
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408662/ https://www.ncbi.nlm.nih.gov/pubmed/30881410 http://dx.doi.org/10.12669/pjms.35.1.55 |
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author | Rahamathulla, Mohamudha Parveen |
author_facet | Rahamathulla, Mohamudha Parveen |
author_sort | Rahamathulla, Mohamudha Parveen |
collection | PubMed |
description | BACKGROUND AND OBJECTIVE: Brucellosis is an important zoonotic disease and a major public health problem in the Middle East countries including Saudi Arabia. This study has evaluated the seroprevalence of human brucellosis in Wadi Al Dawaser region of Central Saudi Arabia. METHODS: The study was conducted for three years (2015-2018) at Wadi Al Dawaser general hospital. A total of 6721 clinically suspected serum samples were collected over three years and tested by Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT), IgM and IgG ELISA. A standard questionnaire to determine the risk factors were used among patients. RESULTS: Of the total 6721 samples tested, 576 (8.6%) were seropositive for brucellosis. RBPT identified 427 (74.1%), SAT titers 493 (85.6%) cases. IgM ELISA and IgG ELISA were positive for 501 (86.9%) and 558 (96.8%) cases respectively. Among the positive cases, 77.3% were male with the male to female ratio of 3.3:1. Nearly, 82% were Saudi Nationals. Direct contact with domestic animals and consumption of raw milk were the risk factors identified. No seasonal variation was seen. Diagnostic yield of IgM ELISA showed 86.9% sensitivity and 100% specificity; IgG ELISA showed 96.8% sensitivity and 100% specificity. Both IgG and IgM ELISAs showed 100% Positive predictive value, 98.9% and 95.6% Negative predictive value respectively. CONCLUSION: A seroprevalence of 8.6% of human brucellosis was documented from this rural region. This is the first report from Wadi Al Dawaser. Frequent surveillance among risk group, vaccination of livestock, creating awareness and health education among the public and school children are recommended. |
format | Online Article Text |
id | pubmed-6408662 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Professional Medical Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-64086622019-03-15 Seroprevalence of Human Brucellosis in Wadi Al Dawaser region of Saudi Arabia Rahamathulla, Mohamudha Parveen Pak J Med Sci Original Article BACKGROUND AND OBJECTIVE: Brucellosis is an important zoonotic disease and a major public health problem in the Middle East countries including Saudi Arabia. This study has evaluated the seroprevalence of human brucellosis in Wadi Al Dawaser region of Central Saudi Arabia. METHODS: The study was conducted for three years (2015-2018) at Wadi Al Dawaser general hospital. A total of 6721 clinically suspected serum samples were collected over three years and tested by Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT), IgM and IgG ELISA. A standard questionnaire to determine the risk factors were used among patients. RESULTS: Of the total 6721 samples tested, 576 (8.6%) were seropositive for brucellosis. RBPT identified 427 (74.1%), SAT titers 493 (85.6%) cases. IgM ELISA and IgG ELISA were positive for 501 (86.9%) and 558 (96.8%) cases respectively. Among the positive cases, 77.3% were male with the male to female ratio of 3.3:1. Nearly, 82% were Saudi Nationals. Direct contact with domestic animals and consumption of raw milk were the risk factors identified. No seasonal variation was seen. Diagnostic yield of IgM ELISA showed 86.9% sensitivity and 100% specificity; IgG ELISA showed 96.8% sensitivity and 100% specificity. Both IgG and IgM ELISAs showed 100% Positive predictive value, 98.9% and 95.6% Negative predictive value respectively. CONCLUSION: A seroprevalence of 8.6% of human brucellosis was documented from this rural region. This is the first report from Wadi Al Dawaser. Frequent surveillance among risk group, vaccination of livestock, creating awareness and health education among the public and school children are recommended. Professional Medical Publications 2019 /pmc/articles/PMC6408662/ /pubmed/30881410 http://dx.doi.org/10.12669/pjms.35.1.55 Text en Copyright: © Pakistan Journal of Medical Sciences http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Rahamathulla, Mohamudha Parveen Seroprevalence of Human Brucellosis in Wadi Al Dawaser region of Saudi Arabia |
title | Seroprevalence of Human Brucellosis in Wadi Al Dawaser region of Saudi Arabia |
title_full | Seroprevalence of Human Brucellosis in Wadi Al Dawaser region of Saudi Arabia |
title_fullStr | Seroprevalence of Human Brucellosis in Wadi Al Dawaser region of Saudi Arabia |
title_full_unstemmed | Seroprevalence of Human Brucellosis in Wadi Al Dawaser region of Saudi Arabia |
title_short | Seroprevalence of Human Brucellosis in Wadi Al Dawaser region of Saudi Arabia |
title_sort | seroprevalence of human brucellosis in wadi al dawaser region of saudi arabia |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408662/ https://www.ncbi.nlm.nih.gov/pubmed/30881410 http://dx.doi.org/10.12669/pjms.35.1.55 |
work_keys_str_mv | AT rahamathullamohamudhaparveen seroprevalenceofhumanbrucellosisinwadialdawaserregionofsaudiarabia |