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Effects of Baihe Dihuang powder on chronic stress depression rat models

PURPOSE: Explore the effect of Baihe Dihuang powder on chronic stress depression rat models. METHODS: Chronic stress depression rat models were established with different stimuli for 21 days. At the same time, the drug was administered for 21 consecutive days. The animals were weighed once a week af...

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Detalles Bibliográficos
Autores principales: Miao, Mingsan, Peng, Mengfan, Chen, Hongbao, Liu, Baosong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408709/
https://www.ncbi.nlm.nih.gov/pubmed/30899175
http://dx.doi.org/10.1016/j.sjbs.2018.12.002
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author Miao, Mingsan
Peng, Mengfan
Chen, Hongbao
Liu, Baosong
author_facet Miao, Mingsan
Peng, Mengfan
Chen, Hongbao
Liu, Baosong
author_sort Miao, Mingsan
collection PubMed
description PURPOSE: Explore the effect of Baihe Dihuang powder on chronic stress depression rat models. METHODS: Chronic stress depression rat models were established with different stimuli for 21 days. At the same time, the drug was administered for 21 consecutive days. The animals were weighed once a week after the start of the formal experiment. On the second day after the end of drug administration, conduct sugar water consumption test and open-filed box experiment, and conduct behavioral observation; At the end of behavioral testing, blood was taken from the eyeball and plasma was separated to measure MDA level and erythrocyte SOD activity; Take brain for homogenate, then measure the contents of 5-HT, NE and DA in brain tissue homogenate; Take the thymus and spleen, stained with 10% formalin fixation, embedding and HE staining, then use microscope to observe the histopathological changes. RESULTS: Chronic stress depression rats model replicated successfully. Each group of given drugs could increase the weight, the consumption of sugar water, and improve the behavioral score, increase erythrocytes SOD activity and decrease MDA level of plasma, increase the content of 5-HT, NE and DA of brain homogenate, and improve the pathological changes of thymus and spleen of chronic stress depression model animals. CONCLUSION: Chronic stress depression rat model replicates successfully. Baihe Dihuang powder can interfere chronic stress depression rats model through different action pathways.
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spelling pubmed-64087092019-03-21 Effects of Baihe Dihuang powder on chronic stress depression rat models Miao, Mingsan Peng, Mengfan Chen, Hongbao Liu, Baosong Saudi J Biol Sci Article PURPOSE: Explore the effect of Baihe Dihuang powder on chronic stress depression rat models. METHODS: Chronic stress depression rat models were established with different stimuli for 21 days. At the same time, the drug was administered for 21 consecutive days. The animals were weighed once a week after the start of the formal experiment. On the second day after the end of drug administration, conduct sugar water consumption test and open-filed box experiment, and conduct behavioral observation; At the end of behavioral testing, blood was taken from the eyeball and plasma was separated to measure MDA level and erythrocyte SOD activity; Take brain for homogenate, then measure the contents of 5-HT, NE and DA in brain tissue homogenate; Take the thymus and spleen, stained with 10% formalin fixation, embedding and HE staining, then use microscope to observe the histopathological changes. RESULTS: Chronic stress depression rats model replicated successfully. Each group of given drugs could increase the weight, the consumption of sugar water, and improve the behavioral score, increase erythrocytes SOD activity and decrease MDA level of plasma, increase the content of 5-HT, NE and DA of brain homogenate, and improve the pathological changes of thymus and spleen of chronic stress depression model animals. CONCLUSION: Chronic stress depression rat model replicates successfully. Baihe Dihuang powder can interfere chronic stress depression rats model through different action pathways. Elsevier 2019-03 2018-12-03 /pmc/articles/PMC6408709/ /pubmed/30899175 http://dx.doi.org/10.1016/j.sjbs.2018.12.002 Text en © 2018 Production and hosting by Elsevier B.V. on behalf of King Saud University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Miao, Mingsan
Peng, Mengfan
Chen, Hongbao
Liu, Baosong
Effects of Baihe Dihuang powder on chronic stress depression rat models
title Effects of Baihe Dihuang powder on chronic stress depression rat models
title_full Effects of Baihe Dihuang powder on chronic stress depression rat models
title_fullStr Effects of Baihe Dihuang powder on chronic stress depression rat models
title_full_unstemmed Effects of Baihe Dihuang powder on chronic stress depression rat models
title_short Effects of Baihe Dihuang powder on chronic stress depression rat models
title_sort effects of baihe dihuang powder on chronic stress depression rat models
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408709/
https://www.ncbi.nlm.nih.gov/pubmed/30899175
http://dx.doi.org/10.1016/j.sjbs.2018.12.002
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