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The research on the mechanism of Tsoong inhibiting for colon cancer
The roots of Codonopis bulleynana Forest ex diels (cbFed), locally known as Tsoong, have been used as a tonic food. Tsoong has wide range of pharmacological effects, including anticancer effects. In the present study, the anticancer activity of Tsoong and its potential molecular mechanisms were inve...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408721/ https://www.ncbi.nlm.nih.gov/pubmed/30899179 http://dx.doi.org/10.1016/j.sjbs.2018.11.007 |
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author | Cao, Yong Dai, Fei Li, Yanmei Jia, Lu Luan, Yunpeng Zhao, Youjie |
author_facet | Cao, Yong Dai, Fei Li, Yanmei Jia, Lu Luan, Yunpeng Zhao, Youjie |
author_sort | Cao, Yong |
collection | PubMed |
description | The roots of Codonopis bulleynana Forest ex diels (cbFed), locally known as Tsoong, have been used as a tonic food. Tsoong has wide range of pharmacological effects, including anticancer effects. In the present study, the anticancer activity of Tsoong and its potential molecular mechanisms were investigated. Using high throughput sequencing the apoptotic pathway was ranked as one of the most important pathways and the differential expressions of apoptosis-related genes such as Casp3, Casp6 and Apaf1 were identified. The following experiments were qRT-PCR which were used to verify the genes. In vitro, cell counting kit-8 (CCK-8) assays and flow cytometry in HCT116 and SW480 colon cancer cell were used to assess the anti‐proliferation and apoptosis-promoting activities of Tsoong. In vivo, the antitumor effect of Tsoong was assessed in colon cancer-bearing nude mice as a xenograft model. H&E staining was performed with oxaliplatin set as a positive control. The results showed that Tsoong up-regulated apoptosis-related genes, inhibited tumor cell proliferation, promoted tumor cellapoptosis in a dose-dependent manner and restrained the growth of colon neoplasm. The effects of a high dose of Tsoong on colon cancer cells were similar to those of oxaliplatin. Our results may ultimately help in the development of diagnostic and therapeutic strategies to control this devastating disease. Therefore, Tsoong may be a promising Chinese herbal compound for development for use in cancer therapy. |
format | Online Article Text |
id | pubmed-6408721 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-64087212019-03-21 The research on the mechanism of Tsoong inhibiting for colon cancer Cao, Yong Dai, Fei Li, Yanmei Jia, Lu Luan, Yunpeng Zhao, Youjie Saudi J Biol Sci Article The roots of Codonopis bulleynana Forest ex diels (cbFed), locally known as Tsoong, have been used as a tonic food. Tsoong has wide range of pharmacological effects, including anticancer effects. In the present study, the anticancer activity of Tsoong and its potential molecular mechanisms were investigated. Using high throughput sequencing the apoptotic pathway was ranked as one of the most important pathways and the differential expressions of apoptosis-related genes such as Casp3, Casp6 and Apaf1 were identified. The following experiments were qRT-PCR which were used to verify the genes. In vitro, cell counting kit-8 (CCK-8) assays and flow cytometry in HCT116 and SW480 colon cancer cell were used to assess the anti‐proliferation and apoptosis-promoting activities of Tsoong. In vivo, the antitumor effect of Tsoong was assessed in colon cancer-bearing nude mice as a xenograft model. H&E staining was performed with oxaliplatin set as a positive control. The results showed that Tsoong up-regulated apoptosis-related genes, inhibited tumor cell proliferation, promoted tumor cellapoptosis in a dose-dependent manner and restrained the growth of colon neoplasm. The effects of a high dose of Tsoong on colon cancer cells were similar to those of oxaliplatin. Our results may ultimately help in the development of diagnostic and therapeutic strategies to control this devastating disease. Therefore, Tsoong may be a promising Chinese herbal compound for development for use in cancer therapy. Elsevier 2019-03 2018-11-15 /pmc/articles/PMC6408721/ /pubmed/30899179 http://dx.doi.org/10.1016/j.sjbs.2018.11.007 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Cao, Yong Dai, Fei Li, Yanmei Jia, Lu Luan, Yunpeng Zhao, Youjie The research on the mechanism of Tsoong inhibiting for colon cancer |
title | The research on the mechanism of Tsoong inhibiting for colon cancer |
title_full | The research on the mechanism of Tsoong inhibiting for colon cancer |
title_fullStr | The research on the mechanism of Tsoong inhibiting for colon cancer |
title_full_unstemmed | The research on the mechanism of Tsoong inhibiting for colon cancer |
title_short | The research on the mechanism of Tsoong inhibiting for colon cancer |
title_sort | research on the mechanism of tsoong inhibiting for colon cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408721/ https://www.ncbi.nlm.nih.gov/pubmed/30899179 http://dx.doi.org/10.1016/j.sjbs.2018.11.007 |
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