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Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study

BACKGROUND: Previous studies from this as well as other research groups suggested that non-invasive chromosome screening (NICS) with embryo culture medium can be used to identify chromosomal ploidy and chromosomal abnormalities. We here report a series of clinical cases utilizing the technology. MET...

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Autores principales: Fang, Rui, Yang, Weimin, Zhao, Xin, Xiong, Fang, Guo, Caiqing, Xiao, Jianping, Chen, Li, Song, Xiaoqing, Wang, Honghua, Chen, Jie, Xiao, Xiao, Yao, Bing, Cai, Li-Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408780/
https://www.ncbi.nlm.nih.gov/pubmed/30849973
http://dx.doi.org/10.1186/s12967-019-1827-1
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author Fang, Rui
Yang, Weimin
Zhao, Xin
Xiong, Fang
Guo, Caiqing
Xiao, Jianping
Chen, Li
Song, Xiaoqing
Wang, Honghua
Chen, Jie
Xiao, Xiao
Yao, Bing
Cai, Li-Yi
author_facet Fang, Rui
Yang, Weimin
Zhao, Xin
Xiong, Fang
Guo, Caiqing
Xiao, Jianping
Chen, Li
Song, Xiaoqing
Wang, Honghua
Chen, Jie
Xiao, Xiao
Yao, Bing
Cai, Li-Yi
author_sort Fang, Rui
collection PubMed
description BACKGROUND: Previous studies from this as well as other research groups suggested that non-invasive chromosome screening (NICS) with embryo culture medium can be used to identify chromosomal ploidy and chromosomal abnormalities. We here report a series of clinical cases utilizing the technology. METHODS: A total of 45 couples underwent in vitro fertilisation during a period between February 2016 and February 2017. Karyotyping revealed normal chromosomes in both partners in 23 couples, and chromosomal rearrangements in at least one partner in 22 couples. Intracytoplasmic sperm injection (ICSI) was used for fertilization. NICS was carried out using embryo culture medium at the blastocyst stage via multiple annealing and looping-based amplification cycles, whole-genome amplification and next-generation sequencing. RESULTS: A total of 413 embryos were obtained; 170 blastocysts were subjected to NICS. The screening showed euploidy in 79 embryos, aneuploidy in 52 embryos, and mosaic ploidy for 33 embryos. The rate of euploidy was comparable in couples with normal karyotype (50.7%; 38/75) vs. chromosomal rearrangement (43.2%; 41/95). A total of 52 euploid embryos (50 oocyte retrieval cycles) were transferred in 43 women. Biochemical pregnancy rate was 72.0% (36/50). Clinical pregnancy rate was 58.0% (29/50). The rate of spontaneous miscarriage was 3/29 (none with chromosomal aneuploidy). A total of 27 healthy babies were delivered. CONCLUSIONS: NICS could identify embryo chromosomal abnormalities in couples either with or without chromosomal rearrangement, with satisfying clinical outcomes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12967-019-1827-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-64087802019-03-21 Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study Fang, Rui Yang, Weimin Zhao, Xin Xiong, Fang Guo, Caiqing Xiao, Jianping Chen, Li Song, Xiaoqing Wang, Honghua Chen, Jie Xiao, Xiao Yao, Bing Cai, Li-Yi J Transl Med Research BACKGROUND: Previous studies from this as well as other research groups suggested that non-invasive chromosome screening (NICS) with embryo culture medium can be used to identify chromosomal ploidy and chromosomal abnormalities. We here report a series of clinical cases utilizing the technology. METHODS: A total of 45 couples underwent in vitro fertilisation during a period between February 2016 and February 2017. Karyotyping revealed normal chromosomes in both partners in 23 couples, and chromosomal rearrangements in at least one partner in 22 couples. Intracytoplasmic sperm injection (ICSI) was used for fertilization. NICS was carried out using embryo culture medium at the blastocyst stage via multiple annealing and looping-based amplification cycles, whole-genome amplification and next-generation sequencing. RESULTS: A total of 413 embryos were obtained; 170 blastocysts were subjected to NICS. The screening showed euploidy in 79 embryos, aneuploidy in 52 embryos, and mosaic ploidy for 33 embryos. The rate of euploidy was comparable in couples with normal karyotype (50.7%; 38/75) vs. chromosomal rearrangement (43.2%; 41/95). A total of 52 euploid embryos (50 oocyte retrieval cycles) were transferred in 43 women. Biochemical pregnancy rate was 72.0% (36/50). Clinical pregnancy rate was 58.0% (29/50). The rate of spontaneous miscarriage was 3/29 (none with chromosomal aneuploidy). A total of 27 healthy babies were delivered. CONCLUSIONS: NICS could identify embryo chromosomal abnormalities in couples either with or without chromosomal rearrangement, with satisfying clinical outcomes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12967-019-1827-1) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-08 /pmc/articles/PMC6408780/ /pubmed/30849973 http://dx.doi.org/10.1186/s12967-019-1827-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Fang, Rui
Yang, Weimin
Zhao, Xin
Xiong, Fang
Guo, Caiqing
Xiao, Jianping
Chen, Li
Song, Xiaoqing
Wang, Honghua
Chen, Jie
Xiao, Xiao
Yao, Bing
Cai, Li-Yi
Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study
title Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study
title_full Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study
title_fullStr Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study
title_full_unstemmed Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study
title_short Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study
title_sort chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408780/
https://www.ncbi.nlm.nih.gov/pubmed/30849973
http://dx.doi.org/10.1186/s12967-019-1827-1
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