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Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study
BACKGROUND: Previous studies from this as well as other research groups suggested that non-invasive chromosome screening (NICS) with embryo culture medium can be used to identify chromosomal ploidy and chromosomal abnormalities. We here report a series of clinical cases utilizing the technology. MET...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408780/ https://www.ncbi.nlm.nih.gov/pubmed/30849973 http://dx.doi.org/10.1186/s12967-019-1827-1 |
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author | Fang, Rui Yang, Weimin Zhao, Xin Xiong, Fang Guo, Caiqing Xiao, Jianping Chen, Li Song, Xiaoqing Wang, Honghua Chen, Jie Xiao, Xiao Yao, Bing Cai, Li-Yi |
author_facet | Fang, Rui Yang, Weimin Zhao, Xin Xiong, Fang Guo, Caiqing Xiao, Jianping Chen, Li Song, Xiaoqing Wang, Honghua Chen, Jie Xiao, Xiao Yao, Bing Cai, Li-Yi |
author_sort | Fang, Rui |
collection | PubMed |
description | BACKGROUND: Previous studies from this as well as other research groups suggested that non-invasive chromosome screening (NICS) with embryo culture medium can be used to identify chromosomal ploidy and chromosomal abnormalities. We here report a series of clinical cases utilizing the technology. METHODS: A total of 45 couples underwent in vitro fertilisation during a period between February 2016 and February 2017. Karyotyping revealed normal chromosomes in both partners in 23 couples, and chromosomal rearrangements in at least one partner in 22 couples. Intracytoplasmic sperm injection (ICSI) was used for fertilization. NICS was carried out using embryo culture medium at the blastocyst stage via multiple annealing and looping-based amplification cycles, whole-genome amplification and next-generation sequencing. RESULTS: A total of 413 embryos were obtained; 170 blastocysts were subjected to NICS. The screening showed euploidy in 79 embryos, aneuploidy in 52 embryos, and mosaic ploidy for 33 embryos. The rate of euploidy was comparable in couples with normal karyotype (50.7%; 38/75) vs. chromosomal rearrangement (43.2%; 41/95). A total of 52 euploid embryos (50 oocyte retrieval cycles) were transferred in 43 women. Biochemical pregnancy rate was 72.0% (36/50). Clinical pregnancy rate was 58.0% (29/50). The rate of spontaneous miscarriage was 3/29 (none with chromosomal aneuploidy). A total of 27 healthy babies were delivered. CONCLUSIONS: NICS could identify embryo chromosomal abnormalities in couples either with or without chromosomal rearrangement, with satisfying clinical outcomes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12967-019-1827-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6408780 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-64087802019-03-21 Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study Fang, Rui Yang, Weimin Zhao, Xin Xiong, Fang Guo, Caiqing Xiao, Jianping Chen, Li Song, Xiaoqing Wang, Honghua Chen, Jie Xiao, Xiao Yao, Bing Cai, Li-Yi J Transl Med Research BACKGROUND: Previous studies from this as well as other research groups suggested that non-invasive chromosome screening (NICS) with embryo culture medium can be used to identify chromosomal ploidy and chromosomal abnormalities. We here report a series of clinical cases utilizing the technology. METHODS: A total of 45 couples underwent in vitro fertilisation during a period between February 2016 and February 2017. Karyotyping revealed normal chromosomes in both partners in 23 couples, and chromosomal rearrangements in at least one partner in 22 couples. Intracytoplasmic sperm injection (ICSI) was used for fertilization. NICS was carried out using embryo culture medium at the blastocyst stage via multiple annealing and looping-based amplification cycles, whole-genome amplification and next-generation sequencing. RESULTS: A total of 413 embryos were obtained; 170 blastocysts were subjected to NICS. The screening showed euploidy in 79 embryos, aneuploidy in 52 embryos, and mosaic ploidy for 33 embryos. The rate of euploidy was comparable in couples with normal karyotype (50.7%; 38/75) vs. chromosomal rearrangement (43.2%; 41/95). A total of 52 euploid embryos (50 oocyte retrieval cycles) were transferred in 43 women. Biochemical pregnancy rate was 72.0% (36/50). Clinical pregnancy rate was 58.0% (29/50). The rate of spontaneous miscarriage was 3/29 (none with chromosomal aneuploidy). A total of 27 healthy babies were delivered. CONCLUSIONS: NICS could identify embryo chromosomal abnormalities in couples either with or without chromosomal rearrangement, with satisfying clinical outcomes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12967-019-1827-1) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-08 /pmc/articles/PMC6408780/ /pubmed/30849973 http://dx.doi.org/10.1186/s12967-019-1827-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Fang, Rui Yang, Weimin Zhao, Xin Xiong, Fang Guo, Caiqing Xiao, Jianping Chen, Li Song, Xiaoqing Wang, Honghua Chen, Jie Xiao, Xiao Yao, Bing Cai, Li-Yi Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study |
title | Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study |
title_full | Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study |
title_fullStr | Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study |
title_full_unstemmed | Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study |
title_short | Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study |
title_sort | chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408780/ https://www.ncbi.nlm.nih.gov/pubmed/30849973 http://dx.doi.org/10.1186/s12967-019-1827-1 |
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