Comparison of Two PCR Assays for Trichomonas vaginalis

PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit(®), using urine collected from four Korean...

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Detalles Bibliográficos
Autores principales: Noh, Chang-Suk, Kim, Sang-Su, Park, Sung-Yul, Moon, Hong-Sang, Hong, Yeonchul, Ryu, Jae-Sook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2019
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409213/
https://www.ncbi.nlm.nih.gov/pubmed/30840796
http://dx.doi.org/10.3347/kjp.2019.57.1.27
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author Noh, Chang-Suk
Kim, Sang-Su
Park, Sung-Yul
Moon, Hong-Sang
Hong, Yeonchul
Ryu, Jae-Sook
author_facet Noh, Chang-Suk
Kim, Sang-Su
Park, Sung-Yul
Moon, Hong-Sang
Hong, Yeonchul
Ryu, Jae-Sook
author_sort Noh, Chang-Suk
collection PubMed
description PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit(®), using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.
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spelling pubmed-64092132019-03-15 Comparison of Two PCR Assays for Trichomonas vaginalis Noh, Chang-Suk Kim, Sang-Su Park, Sung-Yul Moon, Hong-Sang Hong, Yeonchul Ryu, Jae-Sook Korean J Parasitol Brief Communication PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit(®), using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test. The Korean Society for Parasitology and Tropical Medicine 2019-02 2019-02-26 /pmc/articles/PMC6409213/ /pubmed/30840796 http://dx.doi.org/10.3347/kjp.2019.57.1.27 Text en Copyright © 2019 by The Korean Society for Parasitology and Tropical Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Noh, Chang-Suk
Kim, Sang-Su
Park, Sung-Yul
Moon, Hong-Sang
Hong, Yeonchul
Ryu, Jae-Sook
Comparison of Two PCR Assays for Trichomonas vaginalis
title Comparison of Two PCR Assays for Trichomonas vaginalis
title_full Comparison of Two PCR Assays for Trichomonas vaginalis
title_fullStr Comparison of Two PCR Assays for Trichomonas vaginalis
title_full_unstemmed Comparison of Two PCR Assays for Trichomonas vaginalis
title_short Comparison of Two PCR Assays for Trichomonas vaginalis
title_sort comparison of two pcr assays for trichomonas vaginalis
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409213/
https://www.ncbi.nlm.nih.gov/pubmed/30840796
http://dx.doi.org/10.3347/kjp.2019.57.1.27
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