Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep

Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of...

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Autores principales: Tianli, Liu, Xifeng, Wang, Zhenzhong, Tian, Lixia, Wang, Xingxing, Zhang, Jun, Qiao, Qingling, Meng, Shasha, Gong, Ying, Chen, Xuepeng, Cai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2019
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409221/
https://www.ncbi.nlm.nih.gov/pubmed/30840802
http://dx.doi.org/10.3347/kjp.2019.57.1.61
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author Tianli, Liu
Xifeng, Wang
Zhenzhong, Tian
Lixia, Wang
Xingxing, Zhang
Jun, Qiao
Qingling, Meng
Shasha, Gong
Ying, Chen
Xuepeng, Cai
author_facet Tianli, Liu
Xifeng, Wang
Zhenzhong, Tian
Lixia, Wang
Xingxing, Zhang
Jun, Qiao
Qingling, Meng
Shasha, Gong
Ying, Chen
Xuepeng, Cai
author_sort Tianli, Liu
collection PubMed
description Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.
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spelling pubmed-64092212019-03-15 Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep Tianli, Liu Xifeng, Wang Zhenzhong, Tian Lixia, Wang Xingxing, Zhang Jun, Qiao Qingling, Meng Shasha, Gong Ying, Chen Xuepeng, Cai Korean J Parasitol Brief Communication Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep. The Korean Society for Parasitology and Tropical Medicine 2019-02 2019-02-26 /pmc/articles/PMC6409221/ /pubmed/30840802 http://dx.doi.org/10.3347/kjp.2019.57.1.61 Text en Copyright © 2019 by The Korean Society for Parasitology and Tropical Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Tianli, Liu
Xifeng, Wang
Zhenzhong, Tian
Lixia, Wang
Xingxing, Zhang
Jun, Qiao
Qingling, Meng
Shasha, Gong
Ying, Chen
Xuepeng, Cai
Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep
title Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep
title_full Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep
title_fullStr Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep
title_full_unstemmed Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep
title_short Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep
title_sort multi-epitope fusion protein eg mefag-1 as a serodiagnostic candidate for cystic echinococcosis in sheep
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409221/
https://www.ncbi.nlm.nih.gov/pubmed/30840802
http://dx.doi.org/10.3347/kjp.2019.57.1.61
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