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Nine Cases of Methanogenic Archaea in Refractory Sinusitis, an Emerging Clinical Entity

The authors report the cases of 9 patients eventually diagnosed with methanogenic archaea refractory or recalcitrant chronic rhinosinusitis, a condition known to involve various anaerobic bacteria but in which the role of methanogenic archaea is unknown. The authors retrospectively searched these mi...

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Autores principales: Sogodogo, Elisabeth, Fellag, Mustapha, Loukil, Ahmed, Nkamga, Vanessa Demonfort, Michel, Justin, Dessi, Patrick, Fournier, Pierre-Edouard, Drancourt, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409293/
https://www.ncbi.nlm.nih.gov/pubmed/30886840
http://dx.doi.org/10.3389/fpubh.2019.00038
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author Sogodogo, Elisabeth
Fellag, Mustapha
Loukil, Ahmed
Nkamga, Vanessa Demonfort
Michel, Justin
Dessi, Patrick
Fournier, Pierre-Edouard
Drancourt, Michel
author_facet Sogodogo, Elisabeth
Fellag, Mustapha
Loukil, Ahmed
Nkamga, Vanessa Demonfort
Michel, Justin
Dessi, Patrick
Fournier, Pierre-Edouard
Drancourt, Michel
author_sort Sogodogo, Elisabeth
collection PubMed
description The authors report the cases of 9 patients eventually diagnosed with methanogenic archaea refractory or recalcitrant chronic rhinosinusitis, a condition known to involve various anaerobic bacteria but in which the role of methanogenic archaea is unknown. The authors retrospectively searched these microorganisms by PCR in surgically-collected sinusal pus specimens from patients diagnosed with refractory sinusitis, defined by the persistance of sinus inflammation and related-symptoms for more than 12 weeks despite appropriate treatment. Of the 116 tested sinus surgical specimens, 12 (10.3%) from 9 patients (six females, three males; aged 20–71 years) were PCR-positive. These specimens were further investigated by fluorescence in-situ hybridization, PCR amplicon-sequencing and culture. Methanobrevibacter smithii was documented in four patients and Methanobrevibacter oralis in another four, one of whom was also culture-positive. They were associated with a mixed flora including Gram-positive and Gram-negative bacteria. In the latter patient, “Methanobrevibacter massiliense” was the sole microorganism detected. These results highlight methanogenic archaea as being part of a mixed anaerobic flora involved in refractory sinusitis, and suggest that the treatment of this condition should include an antibiotic active against methanogens, notably a nitroimidazole derivative.
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spelling pubmed-64092932019-03-18 Nine Cases of Methanogenic Archaea in Refractory Sinusitis, an Emerging Clinical Entity Sogodogo, Elisabeth Fellag, Mustapha Loukil, Ahmed Nkamga, Vanessa Demonfort Michel, Justin Dessi, Patrick Fournier, Pierre-Edouard Drancourt, Michel Front Public Health Public Health The authors report the cases of 9 patients eventually diagnosed with methanogenic archaea refractory or recalcitrant chronic rhinosinusitis, a condition known to involve various anaerobic bacteria but in which the role of methanogenic archaea is unknown. The authors retrospectively searched these microorganisms by PCR in surgically-collected sinusal pus specimens from patients diagnosed with refractory sinusitis, defined by the persistance of sinus inflammation and related-symptoms for more than 12 weeks despite appropriate treatment. Of the 116 tested sinus surgical specimens, 12 (10.3%) from 9 patients (six females, three males; aged 20–71 years) were PCR-positive. These specimens were further investigated by fluorescence in-situ hybridization, PCR amplicon-sequencing and culture. Methanobrevibacter smithii was documented in four patients and Methanobrevibacter oralis in another four, one of whom was also culture-positive. They were associated with a mixed flora including Gram-positive and Gram-negative bacteria. In the latter patient, “Methanobrevibacter massiliense” was the sole microorganism detected. These results highlight methanogenic archaea as being part of a mixed anaerobic flora involved in refractory sinusitis, and suggest that the treatment of this condition should include an antibiotic active against methanogens, notably a nitroimidazole derivative. Frontiers Media S.A. 2019-03-04 /pmc/articles/PMC6409293/ /pubmed/30886840 http://dx.doi.org/10.3389/fpubh.2019.00038 Text en Copyright © 2019 Sogodogo, Fellag, Loukil, Nkamga, Michel, Dessi, Fournier and Drancourt. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
Sogodogo, Elisabeth
Fellag, Mustapha
Loukil, Ahmed
Nkamga, Vanessa Demonfort
Michel, Justin
Dessi, Patrick
Fournier, Pierre-Edouard
Drancourt, Michel
Nine Cases of Methanogenic Archaea in Refractory Sinusitis, an Emerging Clinical Entity
title Nine Cases of Methanogenic Archaea in Refractory Sinusitis, an Emerging Clinical Entity
title_full Nine Cases of Methanogenic Archaea in Refractory Sinusitis, an Emerging Clinical Entity
title_fullStr Nine Cases of Methanogenic Archaea in Refractory Sinusitis, an Emerging Clinical Entity
title_full_unstemmed Nine Cases of Methanogenic Archaea in Refractory Sinusitis, an Emerging Clinical Entity
title_short Nine Cases of Methanogenic Archaea in Refractory Sinusitis, an Emerging Clinical Entity
title_sort nine cases of methanogenic archaea in refractory sinusitis, an emerging clinical entity
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409293/
https://www.ncbi.nlm.nih.gov/pubmed/30886840
http://dx.doi.org/10.3389/fpubh.2019.00038
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