Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis

It is generally considered that mouse embryonic stem cell (ESC) differentiation into blood cells in vitro recapitulates yolk sac (YS) hematopoiesis. As such, similar to YS-derived B-progenitors, we demonstrate here that ESC-derived B-progenitors differentiate into B-1 and marginal zone B cells, but...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Yang, Kobayashi, Michihiro, Azevedo Portilho, Nathalia, Mishra, Akansha, Gao, Hongyu, Liu, Yunlong, Wenzel, Pamela, Davis, Brian, Yoder, Mervin C., Yoshimoto, Momoko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409422/
https://www.ncbi.nlm.nih.gov/pubmed/30745034
http://dx.doi.org/10.1016/j.stemcr.2019.01.006
_version_ 1783401967839608832
author Lin, Yang
Kobayashi, Michihiro
Azevedo Portilho, Nathalia
Mishra, Akansha
Gao, Hongyu
Liu, Yunlong
Wenzel, Pamela
Davis, Brian
Yoder, Mervin C.
Yoshimoto, Momoko
author_facet Lin, Yang
Kobayashi, Michihiro
Azevedo Portilho, Nathalia
Mishra, Akansha
Gao, Hongyu
Liu, Yunlong
Wenzel, Pamela
Davis, Brian
Yoder, Mervin C.
Yoshimoto, Momoko
author_sort Lin, Yang
collection PubMed
description It is generally considered that mouse embryonic stem cell (ESC) differentiation into blood cells in vitro recapitulates yolk sac (YS) hematopoiesis. As such, similar to YS-derived B-progenitors, we demonstrate here that ESC-derived B-progenitors differentiate into B-1 and marginal zone B cells, but not B-2 cells in immunodeficient mice after transplantation. ESC-derived B-1 cells were maintained in the recipients for more than 6 months, secreting natural IgM antibodies in vivo. Gene expression profiling displayed a close relationship between ESC- and YS-derived B-1 progenitors. Because there are no hematopoietic stem cells (HSCs) detectable in our ESC differentiation culture, successful long-term engraftment of ESC-derived functional B-1 cells supports the presence of HSC-independent B-1 cell development.
format Online
Article
Text
id pubmed-6409422
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-64094222019-03-21 Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis Lin, Yang Kobayashi, Michihiro Azevedo Portilho, Nathalia Mishra, Akansha Gao, Hongyu Liu, Yunlong Wenzel, Pamela Davis, Brian Yoder, Mervin C. Yoshimoto, Momoko Stem Cell Reports Article It is generally considered that mouse embryonic stem cell (ESC) differentiation into blood cells in vitro recapitulates yolk sac (YS) hematopoiesis. As such, similar to YS-derived B-progenitors, we demonstrate here that ESC-derived B-progenitors differentiate into B-1 and marginal zone B cells, but not B-2 cells in immunodeficient mice after transplantation. ESC-derived B-1 cells were maintained in the recipients for more than 6 months, secreting natural IgM antibodies in vivo. Gene expression profiling displayed a close relationship between ESC- and YS-derived B-1 progenitors. Because there are no hematopoietic stem cells (HSCs) detectable in our ESC differentiation culture, successful long-term engraftment of ESC-derived functional B-1 cells supports the presence of HSC-independent B-1 cell development. Elsevier 2019-02-07 /pmc/articles/PMC6409422/ /pubmed/30745034 http://dx.doi.org/10.1016/j.stemcr.2019.01.006 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Lin, Yang
Kobayashi, Michihiro
Azevedo Portilho, Nathalia
Mishra, Akansha
Gao, Hongyu
Liu, Yunlong
Wenzel, Pamela
Davis, Brian
Yoder, Mervin C.
Yoshimoto, Momoko
Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis
title Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis
title_full Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis
title_fullStr Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis
title_full_unstemmed Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis
title_short Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis
title_sort long-term engraftment of esc-derived b-1 progenitor cells supports hsc-independent lymphopoiesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409422/
https://www.ncbi.nlm.nih.gov/pubmed/30745034
http://dx.doi.org/10.1016/j.stemcr.2019.01.006
work_keys_str_mv AT linyang longtermengraftmentofescderivedb1progenitorcellssupportshscindependentlymphopoiesis
AT kobayashimichihiro longtermengraftmentofescderivedb1progenitorcellssupportshscindependentlymphopoiesis
AT azevedoportilhonathalia longtermengraftmentofescderivedb1progenitorcellssupportshscindependentlymphopoiesis
AT mishraakansha longtermengraftmentofescderivedb1progenitorcellssupportshscindependentlymphopoiesis
AT gaohongyu longtermengraftmentofescderivedb1progenitorcellssupportshscindependentlymphopoiesis
AT liuyunlong longtermengraftmentofescderivedb1progenitorcellssupportshscindependentlymphopoiesis
AT wenzelpamela longtermengraftmentofescderivedb1progenitorcellssupportshscindependentlymphopoiesis
AT davisbrian longtermengraftmentofescderivedb1progenitorcellssupportshscindependentlymphopoiesis
AT yodermervinc longtermengraftmentofescderivedb1progenitorcellssupportshscindependentlymphopoiesis
AT yoshimotomomoko longtermengraftmentofescderivedb1progenitorcellssupportshscindependentlymphopoiesis

Ejemplares similares