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Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides
Partially acetylated chitosan oligosaccharides (COS), which consists of N-acetylglucosamine (GlcNAc) and glucosamine (GlcN) residues, is a structurally complex biopolymer with a variety of biological activities. Therefore, it is challenging to elucidate acetylation patterns and the molecular structu...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409515/ https://www.ncbi.nlm.nih.gov/pubmed/30678277 http://dx.doi.org/10.3390/md17020074 |
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author | Zhu, Xian-Yu Zhao, Yong Zhang, Huai-Dong Wang, Wen-Xia Cong, Hai-Hua Yin, Heng |
author_facet | Zhu, Xian-Yu Zhao, Yong Zhang, Huai-Dong Wang, Wen-Xia Cong, Hai-Hua Yin, Heng |
author_sort | Zhu, Xian-Yu |
collection | PubMed |
description | Partially acetylated chitosan oligosaccharides (COS), which consists of N-acetylglucosamine (GlcNAc) and glucosamine (GlcN) residues, is a structurally complex biopolymer with a variety of biological activities. Therefore, it is challenging to elucidate acetylation patterns and the molecular structure-function relationship of COS. Herein, the detailed deacetylation pattern of chitin deacetylase from Saccharomyces cerevisiae, ScCDA(2), was studied. Which solves the randomization of acetylation patterns during COS produced by chemical. ScCDA(2) also exhibits about 8% and 20% deacetylation activity on crystalline chitin and colloid chitin, respectively. Besides, a method for separating and detecting partially acetylated chitosan oligosaccharides by high performance liquid chromatography and electrospray ionization mass spectrometry (HPLC-ESI-MS) system has been developed, which is fast and convenient, and can be monitored online. Mass spectrometry sequencing revealed that ScCDA(2) produced COS with specific acetylation patterns of DAAA, ADAA, AADA, DDAA, DADA, ADDA and DDDA, respectively. ScCDA(2) does not deacetylate the GlcNAc unit that is closest to the reducing end of the oligomer furthermore ScCDA(2) has a multiple-attack deacetylation mechanism on chitin oligosaccharides. This specific mode of action significantly enriches the existing limited library of chitin deacetylase deacetylation patterns. This fully defined COS may be used in the study of COS structure and function. |
format | Online Article Text |
id | pubmed-6409515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64095152019-03-29 Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides Zhu, Xian-Yu Zhao, Yong Zhang, Huai-Dong Wang, Wen-Xia Cong, Hai-Hua Yin, Heng Mar Drugs Article Partially acetylated chitosan oligosaccharides (COS), which consists of N-acetylglucosamine (GlcNAc) and glucosamine (GlcN) residues, is a structurally complex biopolymer with a variety of biological activities. Therefore, it is challenging to elucidate acetylation patterns and the molecular structure-function relationship of COS. Herein, the detailed deacetylation pattern of chitin deacetylase from Saccharomyces cerevisiae, ScCDA(2), was studied. Which solves the randomization of acetylation patterns during COS produced by chemical. ScCDA(2) also exhibits about 8% and 20% deacetylation activity on crystalline chitin and colloid chitin, respectively. Besides, a method for separating and detecting partially acetylated chitosan oligosaccharides by high performance liquid chromatography and electrospray ionization mass spectrometry (HPLC-ESI-MS) system has been developed, which is fast and convenient, and can be monitored online. Mass spectrometry sequencing revealed that ScCDA(2) produced COS with specific acetylation patterns of DAAA, ADAA, AADA, DDAA, DADA, ADDA and DDDA, respectively. ScCDA(2) does not deacetylate the GlcNAc unit that is closest to the reducing end of the oligomer furthermore ScCDA(2) has a multiple-attack deacetylation mechanism on chitin oligosaccharides. This specific mode of action significantly enriches the existing limited library of chitin deacetylase deacetylation patterns. This fully defined COS may be used in the study of COS structure and function. MDPI 2019-01-22 /pmc/articles/PMC6409515/ /pubmed/30678277 http://dx.doi.org/10.3390/md17020074 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhu, Xian-Yu Zhao, Yong Zhang, Huai-Dong Wang, Wen-Xia Cong, Hai-Hua Yin, Heng Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides |
title | Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides |
title_full | Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides |
title_fullStr | Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides |
title_full_unstemmed | Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides |
title_short | Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides |
title_sort | characterization of the specific mode of action of a chitin deacetylase and separation of the partially acetylated chitosan oligosaccharides |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409515/ https://www.ncbi.nlm.nih.gov/pubmed/30678277 http://dx.doi.org/10.3390/md17020074 |
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