Cargando…

Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection

The proteins IFITM1, IFITM2, and IFITM3 are host effectors against a broad range of RNA viruses whose roles in classical swine fever virus (CSFV) infection had not yet been reported. We investigated the effect of these proteins on CSFV replication in mammalian cells. The proteins were overexpressed...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Cheng, Zheng, Hongqing, Wang, Yifan, Dong, Wang, Liu, Yaru, Zhang, Liang, Zhang, Yanming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409519/
https://www.ncbi.nlm.nih.gov/pubmed/30704088
http://dx.doi.org/10.3390/v11020126
_version_ 1783401989355339776
author Li, Cheng
Zheng, Hongqing
Wang, Yifan
Dong, Wang
Liu, Yaru
Zhang, Liang
Zhang, Yanming
author_facet Li, Cheng
Zheng, Hongqing
Wang, Yifan
Dong, Wang
Liu, Yaru
Zhang, Liang
Zhang, Yanming
author_sort Li, Cheng
collection PubMed
description The proteins IFITM1, IFITM2, and IFITM3 are host effectors against a broad range of RNA viruses whose roles in classical swine fever virus (CSFV) infection had not yet been reported. We investigated the effect of these proteins on CSFV replication in mammalian cells. The proteins were overexpressed and silenced using lentiviruses. Confocal microscopy was used to determine the distribution of these proteins in the cells, and immunofluorescence colocalization analysis was used to evaluate the relationship between IFITMs and the CSFV endosomal pathway, including early endosomes, late endosomes, and lysosomes. IFITM1, IFITM2, or IFITM3 overexpression significantly inhibited CSFV replication, whereas protein knockdown enhanced CSFV replication. In porcine alveolar macrophages (PAMs), IFITM1 was mainly located at the cell surface, whereas IFITM2 and IFITM3 were mainly located in the cytoplasm. Following CSFV infection, the distribution of IFITM1 changed. IFITM1, IFITM2, and IFITM3 colocalization with Lamp1, IFITM2 with Rab5 and Rab7, and IFITM3 with Rab7 were observed in CSFV-infected cells. Collectively, these results provide insights into the possible mechanisms associated with the anti-CSFV action of the IFITM family.
format Online
Article
Text
id pubmed-6409519
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64095192019-04-01 Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection Li, Cheng Zheng, Hongqing Wang, Yifan Dong, Wang Liu, Yaru Zhang, Liang Zhang, Yanming Viruses Article The proteins IFITM1, IFITM2, and IFITM3 are host effectors against a broad range of RNA viruses whose roles in classical swine fever virus (CSFV) infection had not yet been reported. We investigated the effect of these proteins on CSFV replication in mammalian cells. The proteins were overexpressed and silenced using lentiviruses. Confocal microscopy was used to determine the distribution of these proteins in the cells, and immunofluorescence colocalization analysis was used to evaluate the relationship between IFITMs and the CSFV endosomal pathway, including early endosomes, late endosomes, and lysosomes. IFITM1, IFITM2, or IFITM3 overexpression significantly inhibited CSFV replication, whereas protein knockdown enhanced CSFV replication. In porcine alveolar macrophages (PAMs), IFITM1 was mainly located at the cell surface, whereas IFITM2 and IFITM3 were mainly located in the cytoplasm. Following CSFV infection, the distribution of IFITM1 changed. IFITM1, IFITM2, and IFITM3 colocalization with Lamp1, IFITM2 with Rab5 and Rab7, and IFITM3 with Rab7 were observed in CSFV-infected cells. Collectively, these results provide insights into the possible mechanisms associated with the anti-CSFV action of the IFITM family. MDPI 2019-01-30 /pmc/articles/PMC6409519/ /pubmed/30704088 http://dx.doi.org/10.3390/v11020126 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Cheng
Zheng, Hongqing
Wang, Yifan
Dong, Wang
Liu, Yaru
Zhang, Liang
Zhang, Yanming
Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection
title Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection
title_full Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection
title_fullStr Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection
title_full_unstemmed Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection
title_short Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection
title_sort antiviral role of ifitm proteins in classical swine fever virus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409519/
https://www.ncbi.nlm.nih.gov/pubmed/30704088
http://dx.doi.org/10.3390/v11020126
work_keys_str_mv AT licheng antiviralroleofifitmproteinsinclassicalswinefevervirusinfection
AT zhenghongqing antiviralroleofifitmproteinsinclassicalswinefevervirusinfection
AT wangyifan antiviralroleofifitmproteinsinclassicalswinefevervirusinfection
AT dongwang antiviralroleofifitmproteinsinclassicalswinefevervirusinfection
AT liuyaru antiviralroleofifitmproteinsinclassicalswinefevervirusinfection
AT zhangliang antiviralroleofifitmproteinsinclassicalswinefevervirusinfection
AT zhangyanming antiviralroleofifitmproteinsinclassicalswinefevervirusinfection