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Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection
The proteins IFITM1, IFITM2, and IFITM3 are host effectors against a broad range of RNA viruses whose roles in classical swine fever virus (CSFV) infection had not yet been reported. We investigated the effect of these proteins on CSFV replication in mammalian cells. The proteins were overexpressed...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409519/ https://www.ncbi.nlm.nih.gov/pubmed/30704088 http://dx.doi.org/10.3390/v11020126 |
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author | Li, Cheng Zheng, Hongqing Wang, Yifan Dong, Wang Liu, Yaru Zhang, Liang Zhang, Yanming |
author_facet | Li, Cheng Zheng, Hongqing Wang, Yifan Dong, Wang Liu, Yaru Zhang, Liang Zhang, Yanming |
author_sort | Li, Cheng |
collection | PubMed |
description | The proteins IFITM1, IFITM2, and IFITM3 are host effectors against a broad range of RNA viruses whose roles in classical swine fever virus (CSFV) infection had not yet been reported. We investigated the effect of these proteins on CSFV replication in mammalian cells. The proteins were overexpressed and silenced using lentiviruses. Confocal microscopy was used to determine the distribution of these proteins in the cells, and immunofluorescence colocalization analysis was used to evaluate the relationship between IFITMs and the CSFV endosomal pathway, including early endosomes, late endosomes, and lysosomes. IFITM1, IFITM2, or IFITM3 overexpression significantly inhibited CSFV replication, whereas protein knockdown enhanced CSFV replication. In porcine alveolar macrophages (PAMs), IFITM1 was mainly located at the cell surface, whereas IFITM2 and IFITM3 were mainly located in the cytoplasm. Following CSFV infection, the distribution of IFITM1 changed. IFITM1, IFITM2, and IFITM3 colocalization with Lamp1, IFITM2 with Rab5 and Rab7, and IFITM3 with Rab7 were observed in CSFV-infected cells. Collectively, these results provide insights into the possible mechanisms associated with the anti-CSFV action of the IFITM family. |
format | Online Article Text |
id | pubmed-6409519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64095192019-04-01 Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection Li, Cheng Zheng, Hongqing Wang, Yifan Dong, Wang Liu, Yaru Zhang, Liang Zhang, Yanming Viruses Article The proteins IFITM1, IFITM2, and IFITM3 are host effectors against a broad range of RNA viruses whose roles in classical swine fever virus (CSFV) infection had not yet been reported. We investigated the effect of these proteins on CSFV replication in mammalian cells. The proteins were overexpressed and silenced using lentiviruses. Confocal microscopy was used to determine the distribution of these proteins in the cells, and immunofluorescence colocalization analysis was used to evaluate the relationship between IFITMs and the CSFV endosomal pathway, including early endosomes, late endosomes, and lysosomes. IFITM1, IFITM2, or IFITM3 overexpression significantly inhibited CSFV replication, whereas protein knockdown enhanced CSFV replication. In porcine alveolar macrophages (PAMs), IFITM1 was mainly located at the cell surface, whereas IFITM2 and IFITM3 were mainly located in the cytoplasm. Following CSFV infection, the distribution of IFITM1 changed. IFITM1, IFITM2, and IFITM3 colocalization with Lamp1, IFITM2 with Rab5 and Rab7, and IFITM3 with Rab7 were observed in CSFV-infected cells. Collectively, these results provide insights into the possible mechanisms associated with the anti-CSFV action of the IFITM family. MDPI 2019-01-30 /pmc/articles/PMC6409519/ /pubmed/30704088 http://dx.doi.org/10.3390/v11020126 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Cheng Zheng, Hongqing Wang, Yifan Dong, Wang Liu, Yaru Zhang, Liang Zhang, Yanming Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection |
title | Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection |
title_full | Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection |
title_fullStr | Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection |
title_full_unstemmed | Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection |
title_short | Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection |
title_sort | antiviral role of ifitm proteins in classical swine fever virus infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409519/ https://www.ncbi.nlm.nih.gov/pubmed/30704088 http://dx.doi.org/10.3390/v11020126 |
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